INVESTIGATION OF THE ANTIMETASTATIC POTENTIAL OF T. SPICATA IN HUMAN BREAST ADENOCARCINOMA CELLS COMBINED WITH STANDARD CHEMOTHERAPY

Yıl 2022, Cilt: 5 Sayı: S-1, 62 - 62, 09.08.2022

Tarık Salman Arzu Yıldırım Yağmur Kaya Hılal Kocdor

https://doi.org/10.26650/JARHS2021-1145563

Öz

Objective: The aim of this study was to determine the effects of hexane extract prepared from Thymbra spicata (TS) on the cytotoxic, antioxidant and metastatic process in human breast adenocarcinoma cell lines (MDA-MB-231, BT-474) in combination with the chemotherapeutic drug Paclitaxel (PAC). Materials And Methods: IC25 and IC50 concentrations of TS and PAC in MDA-MB-231 and BT-474 cell lines were determined by the modified MTT method we developed in our laboratory. Cell motility as an indicator of metastatic potential was evaluated by in vitro wound healing method. MDA was evaluated in cellular cytotoxicity in MB 231 by TAS and TOS analyses. Results: IC25 and IC50 concentrations of TS and PAC in MDA MB 231 and BT-474 cell lines were determined by the modified MTT method we developed in our laboratory. Cell motility as an indicator of metastatic potential was evaluated by in vitro wound healing method. MDA was evaluated in cellular cytotoxicity in MB 231 by TAS and TOS analyses. In wound healing experiments where migration and invasion were tested: MDA MB 231; There was no statistically significant difference in the effect of PAC and TS25/PAC25 doses on cell migration. BT-474; TS and TS25/PAC25 doses were found to be effective in stopping cell migration. Conclusion: The findings revealed the prediction that TS may be among the candidate agents in terms of giving as supportive treatment.

Anahtar Kelimeler

Thymbra spicata, migration, oxidative stress

INVESTIGATION OF THE ANTIMETASTATIC POTENTIAL OF T. SPICATA IN HUMAN BREAST ADENOCARCINOMA CELLS COMBINED WITH STANDARD CHEMOTHERAPY

Öz

Objective: The aim of this study was to determine the effects of hexane extract prepared from Thymbra spicata (TS) on the cytotoxic, antioxidant and
metastatic process in human breast adenocarcinoma cell lines (MDA-MB-231, BT-474) in combination with the chemotherapeutic drug Paclitaxel (PAC).
Materials And Methods: IC25 and IC50 concentrations of TS and PAC in MDA-MB-231 and BT-474 cell lines were determined by the modified MTT method
we developed in our laboratory. Cell motility as an indicator of metastatic potential was evaluated by in vitro wound healing method. MDA was evaluated
in cellular cytotoxicity in MB 231 by TAS and TOS analyses.
Results: IC25 and IC50 concentrations of TS and PAC in MDA MB 231 and BT-474 cell lines were determined by the modified MTT method we developed
in our laboratory. Cell motility as an indicator of metastatic potential was evaluated by in vitro wound healing method. MDA was evaluated in cellular
cytotoxicity in MB 231 by TAS and TOS analyses.
In wound healing experiments where migration and invasion were tested: MDA MB 231; There was no statistically significant difference in the effect of
PAC and TS25/PAC25 doses on cell migration. BT-474; TS and TS25/PAC25 doses were found to be effective in stopping cell migration.
Conclusion: The findings revealed the prediction that TS may be among the candidate agents in terms of giving as supportive treatment.

Anahtar Kelimeler

Thymbra spicata, migration, oxidative stress

Kaynakça

• Arayıcı ME YF, Ellidokuz H. Importance of Cancer Registration and its Place in Cancer Control. Sted. 2021;30(5):366-75.