Genoprotective Potential of Total Anthocyanin Extracted from Prunus avium

Yıl 2017, , 1201 - 1209, 01.12.2017

Ahmet Ali Berber , Fatih Sönmez , Nurcan Berber , Taki Demir , Merve Ayşe Doğancı Betül Aygün Hüseyin Aksoy

https://doi.org/10.16984/saufenbilder.304303

Cited By: 1

Öz

Sweet cherries (Prunus avium) are rich in flavonoids such as
anthocyanins that are beneficial for the immune system.  The aim of the present study was to
investigate in vitro antigenotoxicity and anticytotoxicity of total antochyanins
extracted from Prunus avium (TAP) using by chromosomal aberration (CA), single
cell gel electrophoresis (Comet) and micronucleus assay (MN) in human
peripheral lymphocytes. 50, 100, 200 and 400 µg/mL concentrations of TAP was
screened for its protective activity using mytomicin-C and H₂O₂  induced DNA damage in human lymphocytes.
Also, positive, negative and solvent controls were used.  According to the positive control TAP
statistically decreased the abnormal cell percentage and CA/cell in 200 and 400
µg/mL at 24 h treatment and in all concentrations at 48 h. On the other hand,
it was significantly increased the mitotic index in only 200 µg/mL at 24h and
in all concentrations (except 200µg/mL) at 48h. 
Similarly, TAP were reduced MN frequency in all concentrations according
to the positive control. In the comet assay, significant decreases in comet
tail length, tail moment and tail intensity were observed in all
concentrations. According to these results, we demonstrate that TAP has potent
anticytotoxic and antigenotoxic effect against MMC and H₂O₂-
induced genotoxicity.

Anahtar Kelimeler

Anthocyahins, Prunus avium, Antigenotoxicity, Comet, Chromosomal aberration, Micronucleus

Prunus avium’dan ekstrakte edilen total antosiyaninlerin genoprotektif etkisi

Öz

Kiraz (Prunus avium) antosiyanin gibi immün sistem için
faydalı olan flavonoidler açısından oldukça zengin bir bitkidir. Bu çalışmada
amaç Prunus avium’dan ekstrakte edilen total antosiyaninlerin (TAP) in vitro
antigenotoksik ve antisitotoksik etkilerini insan lenfositlerinde kromozomal
anormallik (CA), tek hücre jel elektroforezi (Comet) ve Mikronükleus testleri
(MN) ile değerlendirmektir. Çalışmamızda, insan lenfositlerinde mitomisin-C ve
H₂O₂ ile indüklenen DNA hasarına karşı TAP’ın 50, 100, 200
and 400 µg/mL’lik konsantrasyonlarının koruyucu etkisi gözlemlenmiştir.  Ayrıca, pozitif, negatif ve çözücü kontrol
grupları da dahil edilmiştir.  Pozitif
kontrole göre TAP; 24 saatlik uygulamanın 200 ve 400 µg/mL’lik, 48 saatlik
uygulamanın ise tüm konsantrasyonlarında anormal hücre yüzdesini ve CA/hücre
frekansını istatistiksel olarak anlamlı bir şekilde azaltmıştır. Benzer şekilde
TAP, mitotik indeksi istatistiksel olarak anlamlı bir şekilde 24 saatlik
uygulamanın sadece 200 µg/mL’lik konsantrasyonunda artırırken, 48 saatlik
uygulamanın ise tüm dozlarında (200 µg/mL’lik konsantrasyon hariç) artırmıştır.
Bu sonuçlara paralel olarak TAP, MN frekansını da pozitif kontrole göre tüm
konsantrasyonlarda istatistiksel olarak anlamlı bir şekilde azaltmıştır. Comet
testinde yapılan TAP uygulamasıyla tüm dozlarda kuyruk uzunluğu, kuyruk momenti
ve kuyruk yoğunluğunda da anlamlı bir azalma olduğu gözlemlenmiştir. Elde
edilen bu sonuçlara göre, TAP’ın mitomisin-C ve H₂O₂ gibi
genotoksik ajanlara karşı potansiyel antisitotoksik ve antigenotoksik koruyucu
bir etkiye sahip olduğu gösterilmiştir.

Anahtar Kelimeler

Prunus avium, Comet, Antosiyaninler, Antigenotoksisite, Kromozomal anormallik, Mikronükleus

Kaynakça

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