Hücre Dışı Matriks Proteinlerinin Nöronlarda Nörit Uzaması ve Dallanmasına Etkileri

Öz

Gelişimin tamamlanmasından sonra periferik sinir sisteminin rejenerasyonu sürecindeki rollerini araştırmak amacıyla bu çalışmada izole nöron kültürlerinde hücre dışı matriks proteinlerinden Laminin, Kollajen Tip 1, Kollajen Tip 4 ve Fibronektinin nörit sayısı, uzunluğu ve dallanmasına olan etkileri incelendi. Bu amaçla ketamin anestezisi altında sakrifiye edilen hayvanlardan arka kök gangliyonları çıkartılarak rutin kültür yöntemleri kullanılarak izole nöron kültürleri hazırlandı. Laminin ve Fibronektin hem 24 hem de 48. saatlerde nöronların çıkardıkları nörit sayısını, toplam nörit uzunluğunu ve nörit uzatan nöron sayısını anlamlı olarak artırdı. Kollajen Tip 1 ve Tip 4 ise 48. saatte nörit uzunluklarını artırdı, ancak her bir nöronun çıkarttığı nörit sayısını etkilemedi. Bu sonuçlar hücre dışı matriks proteinlerinden Laminin ve Fibronektinin nöronlarda nörit gelişimini Kollajene göre daha etkili bir şekilde teşvik ettiğini ve nörorejeneratif amaçlı olarak nörit uzamasını artırmada etkili olabileceğini göstermektedir.

Anahtar Kelimeler

• Laminin
• kollajen
• fibronektin
• nörorejenerasyon.

Effects of Extracellular Matrix Proteins on Neurite Extension and Branching in Neurons

Öz

In order to investigate their roles in the regeneration process of the peripheral nervous system after developmental maturation, the effects of extracellular matrix proteins Laminin, Collagen Type I, Collagen Type IV and Fibronectin on neurite number, length and branching were investigated in isolated neuron cultures in this study. For this purpose, dorsal root ganglia were removed from animals sacrificed under ketamine anesthesia and isolated neuron cultures were prepared using routine culture methods. Laminin and Fibronectin significantly increased the number of neurites produced by neurons, total neurite length, and the number of neurite-extending neurons at both 24 and 48 hours. Collagen Type I and Type IV increased neurite length at 48 hours but did not significantly affect the number of neurites produced by each neuron. These results suggest that extracellular matrix proteins Laminin and Fibronectin promote neurite outgrowth in neurons more effectively than collagen and may be effective in increasing neurite extension for neuroregenerative purposes.

Anahtar Kelimeler

• Laminin
• collagen
• fibronectin
• neuroregeneration.

Kaynakça

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