In vitro Investigation of Rutin-Loaded PLGA Nanoparticles on Leishmania infantum Promastigotes

Öz

Leishmaniasis is a group of illnesses occasioned Leishmania (L.) parasites transmitted by the bites of infected female Phlebotominae class flies and it is endemic in 102 countries. It is seen worldwide, particularly in developing countries. In the present study, the antileishmanial efficacy of free rutin and nanoparticles formed by encapsulating flavonoid rutin in a polymer nanoparticle system on Leishmania infantum promastigotes were contrasted. The efficacy of rutin-loaded PLGA nanoparticles (RT)NPs on the proliferation of promastigote form of L. infantum parasites was examined for the first time by counting the in vitro antileishmanial activities of (RT)NPs using the MTT assay and counting on the thoma slide. It has been observed that (RT)NPs significant affect the proliferation of parasites at concentrations of 1000, 750, and 500 μg/ml at 72nd and 96th hours. The viability% value decreased 10-fold at 1000 μg/ml concentration of (RT)NPs. While the IC50 value of promastigote form of L. infantum parasites was 29.2 ± 4.5 μg/ml in the specimens treated with RT at varied concentrations, the IC50 value of promastigote form of L. infantum parasites was found to be 23.0 ± 2.7 µg/ml in the specimens treated with (RT)NPs. It was observed that the absorbance measurements of (RT)NPs were lower compared to RT at concentrations of 1000, 750, and 500 μg/ml at 48th hour.

Anahtar Kelimeler

• Leishmaniasis
• Antileishmanial efficacy
• Flavonoid
• Rutin
• PLGA

Rutin Yüklü PLGA Nanopartiküllerinin Leishmania infantum Promastigotları Üzerinde In vitro İncelenmesi

Öz

Leishmaniasis, enfekte dişi Phlebotominae sınıfı sineklerin ısırmasıyla bulaşan Leishmania (L.) parazitinin neden olduğu bir hastalık grubudur ve 102 ülkede endemiktir. Dünya genelinde bilhassa gelişmekte olan ülkelerde görülmektedir. Bu çalışmada serbest rutinin ve bir polimer nanopartikül sistemi içerisine bir flavonoid olan rutinin enkapsüle edilerek üretilen nanopartiküllerin Leishmania infantum promastigotları üzerindeki antileishmanial etkinliğini karşılaştırıldı. Rutin yüklü PLGA nanopartiküllerinin ((RT)NP) in vitro antileishmanial etkililiklerinin MTT analiziyle ve thoma lamında sayım yapılmasıyla, ilk sefer olarak (RT)NP’lerinin promastigot formdaki L. infantum parazitlerinin çoğalmasına etkisi incelenmiştir. (RT)NP’lerinin parazitlerin çoğalmasına, 72. ve 96. saatteki 1000, 750 ve 500 μg/ml’lik konsantrasyonlarında büyük oranda etki ettiği görülmüştür. %Canlılık değeri, (RT)NP’lerinin 1000 μg/ml’lik konsantrasyonunda 10 kat azalmıştır. Değişik konsantrasyonlarda RT uygulanan örneklerde, promastigot formdaki L. infantum parazitlerinin IC50 değeri 29,2 ± 4,5 μg/ml iken (RT)NP’leri uygulanan örneklerde promastigot formdaki L. infantum parazitlerinin IC50 ölçümünün 23,0 ± 2,7 μg/ml olduğu belirlenmiştir. 48. saatteki 1000, 750 ve 500 μg/ml’lik konsantrasyonlarda (RT)NP’lerinin, RT’e kıyasla absorbans ölçümlerinin daha alçak olduğu görülmüştür.

Anahtar Kelimeler

• Leishmaniasis
• Antileishmanial etkinlik
• Flavonoid
• Rutin
• PLGA

Kaynakça

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