Investigation of The Bioethanol and Antioxidant Potential of Borodinellopsis texensis Grown in Wastewater under Various Copper Concentrations

Öz

Microalgae have the potential to grow at a rapid rate, which allows them to surpass other living creatures in terms of their prevalence in scientific research settings. Through their growth in wastewater, they are able to make a contribution to the treatment of wastewater. In addition, microalgae's metabolic components make them suitable for industrial applications. In this work, we investigated at how copper stress at various concentrations affected the biomass, carbohydrate, bioethanol, and antioxidant activities of Borodinellopsis texensis cultivated in 25% wastewater. The maximum biomass concentration was 0.79 ± 0.02 g/L at control group. In addition, carbohydrate content was the highest in a medium with 0.025 g/L copper, at 0.29 ± 0.02 g/L. The bioethanol productivities of Borodinellopsis texensis were 174.4 mg/g for 0.025 g/L Cu and 141.8 mg/ g for the control. DPPH, CAT, SOD and APX tests were performed to examine the antioxidant activities of Borodinellopsis texensis. The DPPH maximum scavenging, CAT, SOD, and APX activities of Borodinellopsis texensis were 83%, 17.4 µmol/mg, 8.87 µmol/mg, and 32.7 µmol/mg at 0.025 g/L of Cu, respectively. We are going to continue our investigation into the ways in which microalgae react when it is subjected to copper stress in larger reactors in the future with the assistance of this study.

Anahtar Kelimeler

• Borodinellopsis texensis
• Cu stress
• Bioethanol
• Antioxidant activity
• Wastewater

Çeşitli Bakır Konsantrasyonları altında Atıksuda Büyütülen Borodinellopsis texensis’in Biyoetanol ve Antioksidan Potansiyelinin Araştırılması

Öz

Mikroalgler hızlı büyüyebilme özellikleri ile diğer canlıların önüne geçerek bilimsel çalışmalarda sıklıkla kullanılabilirler. Onlar atıksu içerisinde büyütülerek atıksuyunda arıtımına katkı sağlayabilirler. Ayrıca, mikroalgler içerisindeki metabolik bileşikler sayesinde sanayide kullanılabilir. Bu çalışmada biz %25 atıksu içerisinde büyütülen Borodinellopsis texensis'in çeşitli konsantrasyonlardaki bakır stresine karşı biyokütle, karbonhidrat, biyoetanol ve antioksidan aktivitelerine göstermiş olduğu değişimi inceledik. Maksimum biyokütle konsatrasyonu 0,79 ± 0,02 g/L ile kontrol grubunda bulundu. Buna ek olarak, en yüksek karbonhidrat içeriği 0,025 g/L bakır konsantrasyonunda 0,29 ± 0,02 g/L olduğu gözlemlendi. Borodinellopsis texensis’in biyoetanol verimi 0,025 g/L Cu konsatrasyonunda 174.4 mg/g iken, kontolün biyoetanol verimi 141,8 mg/g olarak bulundu. Borodinellopsis texensis'in antioksidan aktivitelerini incelemek için DPPH, CAT, SOD ve APX testleri yapıldı. Borodinellopsis texensis'in maksimum DPPH yakalama aktivitesi %83, CAT aktivitesi 17,4 µmol/mg, SOD aktivitesi 8,87 µmol/mg ve APX aktiviteside 32,7 µmol/mg olarak 0,025 g/L Cu konsantrasyonunda bulundu. Bu çalışmanın yardımıyla gelecekte mikroalglerin daha büyük reaktörlerde bakır stresine maruz kaldığında nasıl tepki verdiği konusundaki araştırmalarımıza devam edeceğiz.

Anahtar Kelimeler

• Borodinellopsis texensis
• Cu stresi
• Biyoetanol
• Antioksidan aktivite
• Atıksu

Kaynakça

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