Çeşitli Azot Kaynaklarını İçeren Atıksularda Büyütülen Hindakia tetrachotoma'nın Biyodizel ve Biyoetanol Potansiyelinin Değerlendirilmesi

Öz

Mikroalgler biyoyakıt dahil birçok alanda kullanılabilirler. Bunun için mikroalglerin biyokütle veriminin arttırılması ve ürünün yüksek miktarlarda üretilmesi gerekir. Bu çalışmada, farklı azot kaynakları içeren atıksu içerisinde büyütülen Hindakia tetrachotoma mikroalginin biyokütle, biyodizel ve biyoetanol potansiyeli araştıldı. Buna ek olarak antioksidan enzim aktiviteleri olan superoksit dismutaz ve katalaz aktiviteleri incelendi. En yüksek biyokütle konsantrasyonu 1487 ± 21 mg/L ile NO₃⁻ içeren örneklerde elde edildi. Dahası, maksimum lipid yüzdesi 1 mM NO₃⁻ konsantrasyonunda %38 olarak bulundu. En yüksek karbonhidrat yüzdesi 6 mM NO₃⁻ konsantrasyonunda %28 olarak bulundu. En düşük karbonhidrat yüzdesi ise 1 mM NO₂⁻ konsantrasyonunda %15 olarak bulundu. En yüksek biyodizel yüzdesi %118.8 ile 1mM NO₃⁻ konsantrasyonunda ve en yüksek biyoetanol yüzdeside %112 ile 6 mM NO₃⁻ konsantrasyonunda elde edildi. Buna ek olarak, maksimum SOD aktivitesi 1 mM NH₄⁺'de 132 ± 5 U/mg protein olarak bulundu. En düşük SOD aktivitesi ise 6 mM üre konsantrasyonunda 39 ± 3 U/mg protein olarak bulundu. Maksimum CAT aktivitesinin 6 mM NH₄⁺ konsantrasyonunda 120 ± 7 U/mg protein olduğu ölçüldü. Sonuç olarak NO₃⁻ Hindakia tetrachotoma için en kullanışlı azot kaynağı olarak biyodizel ve biyoetanol üretimi içinde uygun olduğu görülmüştür.

Anahtar Kelimeler

• Hindakia tetrachotoma
• Azot
• Biyodizel
• Biyoetanol
• Atıksu

Evaluation of The Biodiesel and Bioethanol Potential of Hindakia tetrachotoma Grown in Wastewater Including Various Nitrogen Sources

Öz

Microalgae can be used in many areas, including biofuel production. This process requires increasing the biomass yield of microalgae and producing high quantities of the product. This study investigated the biomass, biodiesel, and bioethanol potential of Hindakia tetrachotoma microalgae grown in wastewater containing various nitrogen sources. In addition, the antioxidant enzyme activities of superoxide dismutase and catalase were studied. Nitrate-containing samples yielded the highest biomass concentration (1487 ± 21 mg/L). The maximum lipid percentage was 38% at 1 mM NO₃⁻ concentration. In addition, the highest carbohydrate percentage was 28% at a concentration of 6 mM NO₃⁻. The lowest carbohydrate percentage was 15% at a concentration of 1 mM NO₂⁻. Furthermore, the highest biodiesel percentage was 118.8% at 1 mM NO₃⁻ concentration, whereas the highest bioethanol percentage was 112% at 6 mM NO₃⁻ concentration. Also, the maximum SOD activity was 132 ± 5 U/mg proteins at 1 mM NH₄⁺. The lowest SOD activity was 39 ± 3 U/mg proteins at 6 mM urea concentration. The maximum CAT activity was measured to be 120 ± 7 U/mg proteins at a concentration of 6 mM NH₄⁺. As a result, NO₃⁻ was the most effective nitrogen source for Hindakia tetrachotoma microalgae, making it appropriate for biodiesel and bioethanol production.

Anahtar Kelimeler

• Hindakia tetrachotoma
• Nitrogen
• Biodiesel
• Bioethanol
• Wastewater

Kaynakça

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