Electrochemical sensing and fluorescence imaging of E. coli O157:H7 based on aptamer-conjugated semiconducting nanoparticles

Dilek Odacı Demırkol; Kadri Gulec

Electrochemical sensing and fluorescence imaging of E. coli O157:H7 based on aptamer-conjugated semiconducting nanoparticles

Öz

Aptamers are selective molecules against to various sizes of targets from small molecules to mammalian cells. Here, we reported QDs containing electrochemical aptasensor for the detection of E. coli O157:H7. The electrode surfaces were modified by cysteamine (CysN), which has amine and thiol groups, via self-assembled monolayer formation. The carboxyl-functionalized quantum dots (QD) and aptamers (Apt) were conjugated to cysteamine modified gold electrodes. Linear range for E. coli O157:H7 was from 1 to 10² CFU/mL after incubation on CysN/QD/Apt modified Au surfaces. QDs provide fluorescence surface, so that adhesion of cells was followed using fluorescence microscope. Adhered cells were also imaged by scanning electron microscopy. Finally, cell analysis was carried out in real samples.

Anahtar Kelimeler

E. coli O157:H7 sensing,Electrochemical detection; Fluorescence imaging; Quantum dots

Kaynakça

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Ayrıntılar

Birincil Dil

Türkçe

Konular

-

Bölüm

-

Yazarlar

Dilek Odacı Demırkol

Kadri Gulec Bu kişi benim

Yayımlanma Tarihi

5 Ekim 2018

Gönderilme Tarihi

26 Nisan 2018

Kabul Tarihi

-

Yayımlandığı Sayı

Yıl 2018 Cilt: 22

IZ

https://izlik.org/JA84YW85YH

Kaynak Göster

RIS / Bibtex

APA

Odacı Demırkol, D., & Gulec, K. (2018). Electrochemical sensing and fluorescence imaging of E. coli O157:H7 based on aptamer-conjugated semiconducting nanoparticles. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 22, 421-426. https://izlik.org/JA84YW85YH

AMA

1.Odacı Demırkol D, Gulec K. Electrochemical sensing and fluorescence imaging of E. coli O157:H7 based on aptamer-conjugated semiconducting nanoparticles. Süleyman Demirel Üniv. Fen Bilim. Enst. Derg. 2018;22:421-426. https://izlik.org/JA84YW85YH

Chicago

Odacı Demırkol, Dilek, ve Kadri Gulec. 2018. “Electrochemical sensing and fluorescence imaging of E. coli O157:H7 based on aptamer-conjugated semiconducting nanoparticles”. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi 22 (Ekim): 421-26. https://izlik.org/JA84YW85YH.

EndNote

Odacı Demırkol D, Gulec K (01 Ekim 2018) Electrochemical sensing and fluorescence imaging of E. coli O157:H7 based on aptamer-conjugated semiconducting nanoparticles. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi 22 421–426.

IEEE

[1]D. Odacı Demırkol ve K. Gulec, “Electrochemical sensing and fluorescence imaging of E. coli O157:H7 based on aptamer-conjugated semiconducting nanoparticles”, Süleyman Demirel Üniv. Fen Bilim. Enst. Derg., c. 22, ss. 421–426, Eki. 2018, [çevrimiçi]. Erişim adresi: https://izlik.org/JA84YW85YH

ISNAD

Odacı Demırkol, Dilek - Gulec, Kadri. “Electrochemical sensing and fluorescence imaging of E. coli O157:H7 based on aptamer-conjugated semiconducting nanoparticles”. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi 22 (01 Ekim 2018): 421-426. https://izlik.org/JA84YW85YH.

JAMA

1.Odacı Demırkol D, Gulec K. Electrochemical sensing and fluorescence imaging of E. coli O157:H7 based on aptamer-conjugated semiconducting nanoparticles. Süleyman Demirel Üniv. Fen Bilim. Enst. Derg. 2018;22:421–426.

MLA

Odacı Demırkol, Dilek, ve Kadri Gulec. “Electrochemical sensing and fluorescence imaging of E. coli O157:H7 based on aptamer-conjugated semiconducting nanoparticles”. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi, c. 22, Ekim 2018, ss. 421-6, https://izlik.org/JA84YW85YH.

Vancouver

1.Dilek Odacı Demırkol, Kadri Gulec. Electrochemical sensing and fluorescence imaging of E. coli O157:H7 based on aptamer-conjugated semiconducting nanoparticles. Süleyman Demirel Üniv. Fen Bilim. Enst. Derg. [Internet]. 01 Ekim 2018;22:421-6. Erişim adresi: https://izlik.org/JA84YW85YH