Askorbik Asit Tayini için Kil-Protein Nanokompozit Bazlı Elektrokimyasal Sensör

Öz

Bu makalede, montmorillonit-serisin nanokompozit (MMT-Ser NC) ile modifiye edilmiş kalem grafit elektrot (PGE) ile askorbik asit (AA) tayini ilk defa ifade edilmiştir. Nanokompozit yapılar desolvasyon tekniği ile sentezlenmiş ve glutaraldehit (GA) ile çapraz bağlanmıştır. Sentezlenen MMT-Ser NC çeşitli metotlar ile değerlendirilmiştir. Karakterizasyon çalışmaları sonucunda serisinin montmorillonit yapısı ile başarılı bir şekilde bir araya geldiği, MMT-Ser NC’lerin homojen ve eş dağılımlı olup boyutlarının 150 nm ve zeta potansiyellerinin de yaklaşık olarak -27,6 mV olduğu tespit edilmiştir. MMT-Ser NC ile modifiye edilmiş elektrot yüzeyleri dönüşümlü voltametri (CV), elektrokimyasal empedans spektroskopisi (EIS) ve taramalı elektron mikroskopisi (SEM) ile karakterize edilmiştir. Modifiye sensörün optimize koşullar altında kare dalga voltametri (SWV) ile doğrusal çalışma aralığı 10-1000 µM ve gözlenebilme sınırı (LOD) 8 µM olarak bulunmuştur. Tekrar üretilebilirlik için bağıl standart sapma ise % 4,82 (n=6) olarak hesaplanmıştır. İlaç örneği ile gerçek örnek analizi gerçekleştirilmiş ve geri kazanım değerleri % 94,64 ile % 111,2 aralığında elde edilmiştir.

Anahtar Kelimeler

• Askorbik asit,Elektrokimyasal sensör,Montmorillonit-serisin nanokopozit,Kalem grafit elektrot

Clay-Protein Nanocomposite Based Electrochemical Sensor for the Determination of Ascorbic Acid

Öz

This paper describes sensitive ascorbic acid (AA) determination on montmorillonite clay and silk protein sericin nanocomposite (MMT-Ser NC) modified pencil graphite electrode (PGE) for the first time. Nanocomposite structures were synthesized by desolvation technique and cross-linked with glutaraldehyde (GA). The synthesized MMT-Ser NC was evaluated by various methods. As a result of the characterization studies, it was determined that the sericin was successfully converged with the montmorillonite structure, and that the MMT-Ser NCs were homogeneous and uniform, with the size of 150 nm and zeta potentials of approximately -27.6 mV. MMT-Ser NC modified electrodes were evaluated by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and scanning electron microscopy (SEM). Under the optimized conditions, working linear range for the modified sensor was found as 10-1000 µM with square wave voltammetry and the limit of detection (LOD) was found as 8 µM. The relative standard deviation for reproducibility was calculated as % 4.82 (n=6). Real sample analysis was performed with drug samples and the recovery values ranged from 94.64% to 111.2%.

Anahtar Kelimeler

• Ascorbic acid,Electrochemical sensor,Montmorillonite-sericin nanocomposite,Pencil graphite electrode

Kaynakça

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