İnsan Karaciğer Kanserinde İlaç Taşıyıcı Sistem Olarak Serisin-Montmorillonit Kompozit Nanopartiküller: Geliştirilmesi, İlaç salımı, Hücresel Alım ve Sitotoksisite

Öz

Bilimsel ve iyileştiri özelliklerinden dolayı kompozit nanopartiküller artan ilgi görmüşlerdir. Burada, doğal kaynaklardan serisinin biyolojik olarak parçalanabilirliği ve fonksiyonel yüzey çeşitliliği ile montmorillonitin biyolojik uyumluluk ve yüksek adsorpsiyon özelliklerinden yararlanılarak serisin-montmorillonit kompozit nanopartiküller (Ser-Mt NPs) sentezlenmiştir. Kompozit nanopartiküller ilk defa desolvasyon tekniği ile sentezlenmiş ve glutaraldehit ile çapraz bağlanmıştır. Doksorubisin, yükleme ve salım çalışmalarını gerçekleştirmek için model antikanser ilaç olarak seçilmiştir. Zayıflatılmış Toplam Yansıma - Fourier Dönüşümü Kızılötesi Spektroskopisi ve Taramalı Elektron Mikroskobu gibi çeşitli yöntemlerle yapılan kimyasal ve morfolojik karakterizasyon çalışmaları sonrasında Ser-Mt kompozit NP'lerin sitotoksik etkisi, HepG2 (insan karaciğer kanseri hücre hattı) hücreleri üzerinde kalitatif ve kantitatif olarak değerlendirilmiştir. Sonuçlar, yüksek ilaç yükleme kapasitesi, sürdürülebilir ilaç salım özelliği ve kanser hücreleri üzerindeki etkisinin, monodispers, küçük ortalama boyut dağılımı ve iyi çoklu dağılım indeksine sahip Ser-Mt kompozit NP'lerini, kanser terapisi için ilaç dağıtım sistemi olarak iyi bir aday olduğunu açıkça göstermiştir.

Anahtar Kelimeler

• Serisin
• Montmorillonit
• Kompozit nanopartiküller
• Doksorubisin
• İlaç Dağıtım

Sericin-Montmorillonite Composite Nanoparticles as Drug Delivery System in Human Liver Cancer: Development, Drug Release, Cellular Uptake and Cytotoxicity

Öz

Composite nanoparticles obtained increasing interest because of their scientific and curative importance. Herein, sericin-montmorillonite composite nanoparticles (Ser-Mt NPs) were synthesized by taking advantage of the biodegradability and functional surface diversity of sericin, and biocompatibility and high adsorption properties of montmorillonite as natural resources. The composite nanoparticle was obtained by the desolvation technique and crosslinked with glutaraldehyde for the first time. Doxorubicin was selected to be used as a model anticancer drug to perform the loading and release studies. After chemical and morphological characterization studies with various methods such as Fourier Transform Infra-Red Spectroscopy and Electron Microscopy, the cytotoxic effect of Ser-Mt composite NPs were quanlitatively and quantitatively evaluated on HepG2 (human liver cancer cell line) cells. The results obviously exhibited that high drug loading capacity, sustainable drug release property and its effect on cancer cells made Ser-Mt composite NPs as a good candidate as a drug delivery system on cancer therapy with monodisperse, small average size and good polydispersity index.

Anahtar Kelimeler

• Sericin
• Montmorillonite
• Composite nanoparticles
• Doxorubicin
• Drug delivery

Kaynakça

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