Muraena helena Linnaeus, 1758 (Akdeniz Müren Balığı)’nın Mide Mukozasının Histolojik ve Histokimyasal Olarak İncelenmesi

Yıl 2022, , 25 - 34, 25.04.2022

Fatma Kaya , Mehmet Rüştü Özen

https://doi.org/10.19113/sdufenbed.857288

Öz

Çalışmada Muraena helena’nın mide mukozasının, histolojik yapısı ve histokimyasal özelliklerinin belirlenmesi amaçlanmıştır. Bu amaçla Muraena helena (N= 10)’ nın mide bölgelerini oluşturan kardiya, fundus ve pilorustan alınan örnekler incelenmiştir. Otopsi ile alınan doku örnekleri %10 nötral formaldehit solüsyonunda tespit edilmiştir. Rutin histolojik doku takip aşamalarından sonra dokular parafin bloklara alınarak bloklardan 5 µm kalınlığında kesitler hazırlanmıştır. Preparatlar Hematoksilen-Eosin (H-E), Aldehyde Fuchsin (AF), Periodic Acid Schiff (PAS), KOH, Alcian Blue (AB) pH 0.5, AB 0.3 M, AB 0.06 M, AB pH 2.5, AF/AB pH 2.5, AB pH 1.0, PAS/ AB pH 2.5 boyama yöntemleri ile boyanmış ve fotoğraflanmıştır. İncelenen doku kesitlerinde, mide mukozasının tunika mukoza, submukoza, tunika muskularis ve tunika seroza olarak dört katmandan oluştuğu gözlenmiştir. Uygulanan histokimyasal boyama yöntemleri ile kardiya, fundus ve pilorus bölgelerinin güçlü düzeyde sülfatlı asidik, asidik, sülfatlı ve karboksilli asidik mukosubstans özelliği gösterdiği tespit edilmiştir.

Anahtar Kelimeler

Muraena helena, Akdeniz Müreni, Mukosubstans, Mukoza, Histokimya

Teşekkür

Bu çalışma sürecinde çalışmalarıma destek veren danışman hocam Doç. Dr. Mehmet Rüştü ÖZEN’ e teşekkür ederim. Laboratuvar çalışmalarımda çalışma imkanı sunan değerli hocam Doktor Öğretim Üyesi Kenan ÇINAR’ a teşekkür ederim. Araştırma materyali olan Akdeniz Müren balığını yakalayarak bana çalışma konusunda büyük destek veren değerli hocam Akdeniz Üniversitesi Su Ürünleri Fakültesi Öğretim Üyesi Prof. Dr. Mehmet GÖKOĞLU’ na sonsuz teşekkür ederim.

Histological and Histochemical Investigation of the Stomach Mucosa of Muraena helena Linnaeus, 1758 (Mediterranean Moray Fish)

Öz

In this study, it was aimed to determine the histological structure and histochemical properties of gastric mucosa of Muraena helena. For this purpose, samples taken from cardia, fundus and pylorus which from the stomach regions of Muraena helena (N=10) were examined. Tissue samples taken at autopsy were fixed in 10 % neutral formaldehyde solution. After the routine histological tissue following procedure stages, tissues were taken into paraffin blocks and 5 µthick sections were prepared from the blocks. The preparations were stained with Hematoxylin-Eosin (H-E), Aldehyde Fuchsin (AF), Periodic Acid Schiff (PAS), KOH/PAS, Alcian Blue (AB) pH 0.5, AB 0.3 M, AB 0.06 M, AB pH 2.5, AF/AB pH 2.5, AB pH 1.0, PAS/AB pH 2.5 staining methods and photographed. In the tissue sections examined, it was observed that the stomach mucosa consists of four layers as tunica mucosa, submucosa, tunica muscularis and tunica serosa. With the histochemical staining methods applied, it was determined that the cardia, fundus and pylorus regions showed sulphate acidic, acidic sulphate and carboxylated acidic mucosubstances.

Anahtar Kelimeler

Mediterranean morayeel, Muraena helena, Histochemistry, Mucosa, Mukosubstance

Kaynakça

• [1] Eschmeyer, W. N., Fong, J. D. 2015. Speciesbyfamily /subfamily (Erişim Tarihi: 26.09.2019). http://researcharchive.calacademy.org/research/ichthyology/catalog/speciesbyfamily.asp). Electronic version accessed 31Mart 2015.
• [2] Sallami B., Bearez P., Salem M. B., 2016. Age and Growth of Muraena helena (Muraenidae) from the Northcoast of Tunisia. Cybium 40(2), 155-161.
• [3] Jımenez, S., Schoenhut, S., Lozano, I. J., Gonzales, J. A., Sevilla R. G., Dıez, A., Bautısta, J. M. 2007. Morphological, Ecological, and Molecular Analyses Separate Muraena augusti from Muraena helena As A Valid Species. American Society of Ichthyologists and Herpetologists, Copeia 1, 101-113.
• [4] Matic-Skoko, S., Tutman, P., Petric, M., Skaramuca, D., Lusicic, D., Dikic, D., Skaramuca, B. 2011. Mediterranean morayeel Muraena helena (Pisces: Muraenidae): Biological Indicesfor Life History, Aquaticbiology, 13, 275-284.
• [5] Demirağ, Ö. 2013. Su Altında Ön Yargı: Akdeniz Mürenleri. http2://dalalim.com/akdeniz-murenleri/ (Erişim tarihi. 24.12.2018).
• [6] Bosi, G., DiGiancamillo, A., Arrighi, S., Domeneghini, C. 2004. An Immunohistochemical Study on The Neuroendocrine System In The Alimentary Canal of The Brown Trout, Salmo trutta, L., 1758. General and Comparative Endocrinology, 138(2), 166-81.
• [7] Bancroft, J. D. Stevens, A. 1996. Theory and Practice of Histological Techniques. Churchill livingstone, MedicalDiv. Longman Group Ltd. Edinburgh, Londonand New York. 436 p.
• [8] Culling, C. F. A., Reid, P. E., Dunn, W. L., 1976. A new histochemical method for the identification and visualization of both side chain acylated and noacylated sialicacids. Journal of Histochemistry and Cytochemistry, 24, 1225–1230.
• [9] Luna, L. 1982. Manual of Histologic StainingMethods. American Registry of Pathology, McGraw-HillCo. NewYork. p. 251.
• [10] Gomori, G. 1952. Gomori’s Aldehyde Fuchsin stain. In: Cellular Pathology Technique. Butterworths, p. 238–240.
• [11] Lev, R., Spicer, S. S. 1964. Specific Staining of Sulphate Groups with Alcian Blue at low pH. Journal of Histochemistry and Cytochemistry. 12, 309.
• [12] Scott, J. E., Dorling, J. 1965. Differential Staining of Acid Glycosaminogkycans (Mucopolysaccharides) by Alcian Blue in Salt Solutions. Histochemie, 21, 277– 285.
• [13] Mayer, S. 1960. Aldehyde Fuchsin/Alcian Blue. In: Cellular Pathology Technique, Butterworths, p. 233.
• [14] McManus, J. F. A. 1948. Histological and Histochemical Uses of Periodic Acid. Stain Technology, 23, 99–108.
• [15] Mowry, R. W. 1956. Alcian Blue Techniques for The Histochemical Study of Acidic Carbohydrates. Journal of Histochemistry and Cytochemistry, 4, 407–408.
• [16] Al-Hussaini, A. H., Kholy, A.A., 1953. On The Functional Morphology of The Alimentary Tract of Some Omnivorous Fish. Proceedings of the Egyptian Academy of Sciences, 4, 17–39.
• [17] Murray, H. M., Wright, G. M., Goff, G. P. 1994. A Comparative Histological and Histochemical Study of The Stomach from Three Species of Pleuronectid, The Atlantic Halibut, Hippoglossus hippoglossus, The Yellowtail Flounder, Pleuronectes ferruginea, and The Winter Flounder, Pleuronectes americanus. Canadian Journal of Zoology, 72, 1199–1210.
• [18] Caceci, T., El-Habback, H. A., Smith, S. A., Smith, B. J., 1997. The Stomach of Oreochromis niloticus Has Three Regions. Journal of FishBiology, 50 (5), 939-952.
• [19] Çınar, K., Diler, A., Boyacı, Ö., Bilgin, F. 1997. Histological Structure and Histochemical Characteristics of the Stomach of the Salmo trutta macrostigma and Oncorhynchus mykiss. Turkishjournal of Zoology, 21, 503-508.
• [20] Albrecht, M. P., Ferreira, M. F. N., Caramaschi, E. P. 2001. Anatomical Features and Histology of the Digestive Tract of Two Related Reotropical Omnivorous Fishes (Characiformes; Anostomidae). Journal of Fish Biology, 58, 419–430.
• [21] Arellano, J. M., Storch, V., Sarasquete, C. 2001. Histological and Histochemical Observations in The Stomach of The Senegal Sole. Histology and Histopathology, 16, 511–521.
• [22] Suiçmez, M., Ulus, E. 2005. A Study of the Anatomy, Histology and Ultrastructure of the Digestive Tract of Orthrias angorae, Steindachner, 1897. Foliabiologica (Kraków), 53(1-2), 95-100.
• [23] Aksoy, A. 2009. Gökkuşağı alabalığı (Oncorhynchus mykiss) sindirim kanalının postnatal gelişimi ve endokrin hücre kompozisyonu üzerinde histolojik, histokimyasal ve immunohistokimyasal çalışmalar. SDÜ Fen Bilimleri Enstitüsü, Doktora Tezi, 101 s, Isparta.
• [24] Diaz, A. O., Garcia, A. M., Devincenti, C. V., Goldemberg, A. L 2003. Morphological and Histochemical Characterization of the Mucosa of the Digestive Tract in Engraulis anchoita. Anatomia Histologia Embryologia, 32, 341–346.
• [25] Pedini, V., Scocco, P., Radaelli, G., Fagioli, O., Ceccarelli, P., 2001. Carbohydrate Histochemistry of The Alimentary Canal of The ShiDrum, Umbrina cirrosa L. Anatomia Histologia Embriologia, 30, 345–349.
• [26] Rodríguez, A., Gisbert, E., Rodríguez, G., Castelló-Orvay, F. 2005. Histopathological Observations in European Glass Eels (Anguilla anguilla) Reared Under Different Diets and Salinities. Aquaculture, 244(1-4), 203- 214.
• [27] Chatchavalvanich, K., Marcos, R., Poonpirom, J., Thongpan, A., Rocha, E. 2006. Histology of The Digestive Tract of The Freshwater Stingray Himantura signifer Compagno and Roberts, 1982 (Elasmobranchii, Dasyatidae). Anatomy and Embryology, 211(5), 507-518.
• [28] Hellberg, H., Bjerkas, I. 2000. The Anatomy of The Oesophagus, Stomach and Intestine in Common Wolffish (Anarchichas lupus L.): A Basis for Diagnostic Work and Research. Acta Veterinaria Scandinavica, 41, 283-297.
• [29] Carrasson, M., Grau, A., Dopazo, L. R., Crespo, S., 2006. A Histological, Histochemical and Ultrastructural Study of The Digestive Tract of Dentex dentex (Pisces, Sparidae). Histology and Histopathology, 21(4-6), 579-593.
• [30] Díaz, A. O., Garcia, A. M., Figueroa, D. E., Goldemberg, A. L. 2008. Glycoconjugates in the mucosa of the digestive tract of Cynoscion guatucupa. A histochemical study. Acta Histochemica, 110, 76–85.
• [31] Grau, A., Crespo, S., Sarasquete, M. C., González de Canales M. L. 1992. The digestive tract of the amberjack Seriola dumerili, Risso: Light and Scanning lectronmicroscope Study. Journal of FishBiology, 41, 287–303.
• [32] Morrison, C. M., Wright, J. R. 1999. A Study of The Histology of The Digestive Tract of The Nile Tilapia. Journal of Fish Biology, 54, 597–606.
• [33] Şenol, N. 2009. Sazan (Cyprinus carpio) ve Sudak (Stizostedion lucioperca) Balıklarında Gastrointestinal Kanalın Histokimyasal Yapısı ve Bazı Peptidlerin Lokalizasyonu. SDÜ Fen Bilimleri Enstitüsü Biyoloji ABD, Doktora Tezi 76 s. Isparta.
• [34] Diaz, A. O., Garcia, A. M., Figueroa, D. E., Goldemberg, A. L. 2008. The mucosa of digestive tract in Micropogonias furnieri: A light and electron microscope approach. Anatomia Histologia Embryologia, 37, 251–256.
• [35] Elbal, M. T., Agulleiro, M. T. 1986. An histochemical and ultrastructural study of the gut of Sparus auratus (Teleostei). Journal of Submicroscopic Cytology, 18, 2, 335–347.
• [36] Ferri, D., Liquori, G.E., Scillitani, G. 1999. Morphological and Histochemical Variations of Mucous and Oxynticopepticcells in the Stomach of the Seps, Chalcides chalcides. Journal of Anatomy, 194, 71–77.