Aflatoksin B1'in Zebra balıgının (Danio rerio (Hamilton)) Embriyo ve Larvaları Üzerine Olan Toksik Etkileri

Yıl 2007, Cilt: 11 Sayı: 1, 13 - 17, 09.04.2009

Turgay Şişman Yılmaz Yıldırım

Öz

Bu çalısmada, toksik bir küf metaboliti olan Aflatoksin B1 (AFB1)'in Danio rerio embriyolarının gelisimi üzerine olan teratojenik etkileri arastırılmıstır. Artan konsantrasyonlarda AFB1 uygulanan balık embriyolarında çesitli anormallikler 96 saat süreyle gözlenmistir (24hpf, 48 hpf, 72 hpf, 96 hpf). Bu anormallikler Rasisis (gelisimde gerileme), Lordoz (vertebra anormalligi), kardiak ödemi, çesitli vücut kısımlarının olusmamasıdır. AFB1'e maruz kalma süresi ve konsantrasyon arttıkça anormalliklerin görülme sıklıgı ve ölümler de artmıstır. Hemen hemen bütün dozlarda anormallikler gözlenmis ancak kontrol gruplarında hiçbir anormallik gözlenmemistir. Kontrol ve deney grupları arasındaki fark istatistiksel olarak önemli bulunmustur (P<0.05). AFB1'in neden oldugu malformasyonların olası mekanizması tartısılmıstır.

Anahtar Kelimeler

Danio Rerio, AFB1, Teratojenik Etki, Embriyo, Larva

Kaynakça

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