Theodoxus syriacus (Gastropoda: Prosobranchia)'un Nötral Lipit, Fosfolipit ve Total Lipit Yağ Asiti Kompozisyonunun Mevsimsel Değişimi

Yıl 2010, Cilt: 14 Sayı: 1, - , 27.02.2014

İhsan Ekin Mehmet Başhan Rıdvan Şeşen

Öz

Diyarbakır Devegeçidi Köprüsü civarından toplanan tatlısu salyangozu Thedoxus syriacus (Bourguignat, 1853)'un fosfolipit, nötral lipit ve total vücut lipitlerinin mevsimsel yağ asiti değişimi, bir yıl boyunca araştırıldı. Salyangozun tüm vücut lipitleri, ince tabaka kromatografi ile fraksiyonlandı. Fosfolipit, nötral lipit ve total lipit yağ asitleri gaz kromatografi (GC) ve gaz kromatografi- kütle spektrometresi (GC-MS) metodları ile analiz edildi. Lipit analizlerinde, C16:0, C18:0, C18:1<»9, C18:2<»6 ve C18:3<»3 asitler, diğer asitlere göre daha yüksek oranda saptandı. Fosfolipit, nötral lipit ve total lipitte bazı önemli farklılıklar bulundu. Yağ asitlerinin mevsimsel değişimleri, kısmen sıcaklık ve besin ile bağlantılı olduğu görüldü. Total doymuş yağ asiti (â–¡DYA) oranları (fosfolipitte %47.75, nötral lipitte %45.61 ve total lipitte %41.63) yaz döneminde yüksek oranda bulundu. Total tekli doymamış yağ asiti (â–¡TDYA) oranı, fosfolipitte (%35.05) en çok kış mevsiminde, nötral (%34.83) ve total lipitte (%39.10) ise en çok ilkbahar mevsiminde tespit edildi. Total çoklu doymamış yağ asiti (â–¡ÇDYA) oranları (fosfolipitte %55.89, nötral lipitte %59.99 ve total lipitte %50.66) ise tüm analizlerde sonbahar döneminde oldukça yüksek saptandı. Bu yüksek oran C18:2<»6 asitin, tüm analizlerin sonbahar döneminde çok yüksek oranda (%36.42 - %54.30) tespit edilmesinden kaynaklandı.
Anahtar kelimeler: Yağ Asitleri, Fosfolipit, Nötral Lipit, Theodoxus syriacus,Tatlısu Salyangozu

Anahtar Kelimeler

Yağ Asitleri, Fosfolipit, Nötral Lipit, Theodoxus syriacus, Tatlısu Salyangozu

Kaynakça

• Abad, M., Ruiz, C., Martinez, D., Mosquera, G., Sanchez, J.L. 1995. Seasonal Variation of Lipid Classes and Fatty Acids in Flat Oyster Ostrea Edulis From San Cibrian (Galicia, SPAIN). Comp. Biochem. Physiol., 110 C (2), 109-118.
• Ackman, R.G. 1989. Fats and Oils, Pp. 103-137, in Ackman, R.G. (editor), Fatty Acids in Marine Biogenic Lipids. CRC Press, Boca Raton, Florida.
• Alimova, E.K., Astvatzatur’an, A.T., Zharov, L.B. 1975. Medicine , Pp. 280, in Levachev, M.M. (editor), Lipids and fatty Acids in Normal and Some Pathological States. Meditsina, Moscow.
• Bligh, E.G., Dyer, W.J.A. 1959. A Rapid method of total Lipid Extraction and Purification. Can. J. Biochem. Physiol., 37, 911-917.
• Brazao, S., Morais, S., Boaventura, D., Re, P., Narciso, L., Hawkins, S.J. 2003. Spatial and Temporal Variation of the Fatty Acid Composition of Patella Spp. (Gastropoda: Prosobranchia) Soft Bodies and Gonads. Comp. Biochem. Physiol., 136 B, 425-441.
• De Moreno, J.E.A., Pollero, R.J., Moreno,V.J., Brenner, R.R. 1980. Lipids and Fatty Acids of the Mussel (Mytilus platensis d’Orbigny) From South Atlantic waters. J. Exp. Mar. Biol. Ecol., 263-276.
• Dembitsky, V.M., Kashin, A.G., Stefanow, K. 1992. Comparative Investigation of Phospholipids and Fatty Acids of Freshwater Molluscs From Volga River Basin. Comp. Biochem. Physiol., 102B (1), 193-198.
• Dembitsky, V.M., Rezanka, T., Kashin, A.G. 1993. Fatty Acid and Phospholipids Composition of Freshwater Molluscs Anadonta piscinalis and Limnaea fragilis From the River Volga. Comp. Biochem. Physiol.,105B, 3(4), 597-601.
• Dembitsky, V.M., Rezanka,T., Kashin, A.G. 1994. Comparative Study of the Endemic Freshwater Fauna of Lake Baikal-IV. Phospholipids and Fatty Acid Composition of Two Gastropod Molluscs of the Genus Valvata. Comp. Biochem. Physiol., 107B, 325-330.
• Ekman, S. 1953. Zoogeography of the Sea. Plenum Press, London, 417 pp.
• Fried, B., Rao, S.K., Sherma, J. 1992. Fatty Acid Composition (Gastropoda: Planorbidae) Fed Hen’s egg Yolk Versus Physiol.,101A, 351-352. glabrata Leaf Lettuce. Comp. Biochem.
• Fried, B., Rao, K.S., Sherma, J., Huffmani, J.E. 1993. Fatty Acid Composition of Goniobasis virginica, Physa sp. and Viviparus malleatus (Mollusca: Gastropoda) From Lake Musconetcong, New Jersey. Biochem. Syst. and Ecol., 21(8), 809-812.
• Go, J.V., Rezanka, T., Srebnik, M., Dembitsky, V.M. 2002. Variability of Fatty Acid Component of Marine and Freshwater Gastropod Species From the Littoral Zone of the Red Sea, Mediterranean Sea and Sea of Galilee. Biochem. Syst. and Ecol., 30, 819-835.
• Hagar, A.F., Dietz,T.H. 1986. Seasonal Changes in the Lipid Composition of Gill Tissue From the Freshwater Mussel Carunculina Texasensis. Phsiol. Zool., 59 (4), 419-428.
• Holland, D.L. 1978. Lipid Reserve and Energy Metabolism in the Larvae of Benthic Marine İnvertabrates. Adv. Mar. Biol., 14, 85-123.
• Johns, R.B., Nichols, P.D., Perry, G.J. 1980. Fatty Acid Components of Nine Species of Molluscs of the littoral Zone From Australian Waters. Comp. Biochem. Physiol., 65B, 207-214.
• Joseph, J.D. 1982. Lipid composition of Marine and Estuarine Invertebrates. Part II: Mollusca. Prog. Lipid Res., 21, 109-153.
• Kharlamenko, V.I., Zhukova, N.V., Khotimchenko, S.V., Svetashev, V.I., Kamenev, G.M. 1995. Fatty Acids As Markers of Food Sources in A Shallow Water Hydrothermal Ecosystem (Kraternaya Bight, Yankich Island, Kurile Islands). Mar. Ecol. Prog. Ser., 120, 231-241.
• Logue, J.A., Howell, B.R., Bell, J.G., Cossins, A.R. 2000. Diatery n-3 long-Chain Polyunsaturated Fatty Acids Deprivation, Tissue Lipid Composition, Ex Vivo Prostaglandin Production and Stress Tolerance İn Juvenile (Solea solea L.). Lipids, 35, 745-755. 30
• Misra, K.K., Shkrob, I., Rakshit, S., Dembitsky, V.M. 2002. Variability in Fatty Acids and Fatty Aldehydes in Different Organs of Two Prosobranch Gastropod Molluscs. Biochem. Syst. and Ecol., 30, 749-761.
• Misra, S., Ghosh, K.M., Choudhury, A., Dutta, K. A., Pal, K. P. and Ghosh,A., 1985. Fatty Acids From Macoma sp. of Bivalve Mollusc. J. Sci. Food Agric., 36, 1193-1196.
• Pazos, J.A., Ruiz, C., Martin, G.O., Abad, M., Sanchez, L.J. 1996. Seasonal Variation of the Lipit Content And Fatty Acid Composition of Crassostrea gigas Cultured in El Grove, Galicia, N.W. SPAIN. Comp. Biochem. Physiol., 114B (2), 171-179.
• Pazos, J.A., Sanches, L.J., Roman, G., Perez-Parelle, M.L., Abad, M. 2003. Seasonal Changes in Lipid Classes and Fatty Acids Composition in Digestive Gland of Pecten maximus. Comp. Biochem. Physiol., 134B, 367-380.
• Pollero, R.J., Irazu, C.E., Brenner, R.R. 1983. Effect of Sexual Stage On Lipids and Fatty Acids of Diplodon delodontus. Comp. Biochem. Physiol., 76B, 927-931.
• Rudin, D.O. 1982. the Dominant Diseases of Modernized Socities As Omega-3 Essential Fatty Acid Deficiency Syndrome: Substrate Beri-beri. Med. Hypothesses, 8, 17-47.
• Sargent, J.R. 1976. Biochemical and Biophysical Perspectives in Marine Biology, Pp 149-212, in Malin, D.C. and Sargent, J.R. (editors). The Structure, Metabolism and Function of Lipids in Marine Organisms. Academic Press, Londan.
• Stanley, D.W., Howard, R.W. 1998. the Biology of Prostaglandins and Related Eicosanoids in İnvertebrates: Cellular Organismal and Ecological Actions. Am Zool 38: 369-381.
• Stanley-Samuelson, D.W., Dadd, R.H. 1983. Long Chain Polyunsaturated Fatty Acids: Patterns of Occurrence in Insects. Biochemistry, 13, 549-558.
• Wennmalm, A. 1977. Vasodilatory Action of Arachidonic Acid ın Human Following Indomethacin Treatment. Prostaglandins, 13, 809-810.
• Zhukova, N.V. 1991. The Pathway of the Biosynthesis of Non-Methylene-Interrupted Dienoic Fatty Acids in Molluscs. Comp. Biochem. Physiol., 110B, 801- 804. 31