Electrochemical sensing and fluorescence imaging of E. coli O157:H7 based on aptamer-conjugated semiconducting nanoparticles

Yıl 2018, Cilt: 22 Sayı: Özel, 421 - 426, 05.10.2018

Dilek Odacı Demırkol , Kadri Gulec

Öz

Aptamers are selective molecules against to various sizes of targets from small molecules to mammalian cells. Here, we reported QDs containing electrochemical aptasensor for the detection of E. coli O157:H7. The electrode surfaces were modified by cysteamine (CysN), which has amine and thiol groups, via self-assembled monolayer formation. The carboxyl-functionalized quantum dots (QD) and aptamers (Apt) were conjugated to cysteamine modified gold electrodes. Linear range for E. coli O157:H7 was from 1 to 10² CFU/mL after incubation on CysN/QD/Apt modified Au surfaces. QDs provide fluorescence surface, so that adhesion of cells was followed using fluorescence microscope. Adhered cells were also imaged by scanning electron microscopy. Finally, cell analysis was carried out in real samples.

Anahtar Kelimeler

E. coli O157:H7 sensing, Electrochemical detection; Fluorescence imaging; Quantum dots

Kaynakça

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