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Su Sıcaklığının Doktor Balık (Garra rufa) Türünün Kas, Ağız ve Bağırsak Dokularındaki Katalaz Aktivitesi ve Malondialdehit Düzeyleri Üzerine Etkileri

Yıl 2019, Cilt: 23 Sayı: 1, 191 - 194, 01.04.2019
https://doi.org/10.19113/sdufenbed.483082

Öz

Bu çalışmada farklı su
sıcaklıklarında (18°C ve 34°C) beslenen Garra
rufa (doktor balık) türü balıkların bazı dokularında (kas, ağız ve
bağırsak) katalaz (CAT) aktiviteleri ve malondialdehit (MDA) düzeyleri
incelenmiştir. Deney süresince hiç balık ölümü görülmemiştir. Önemlilik
dereceleri farklı olmakla birlikte, 34°C de beslenen doktor balıkların oransal
boy artışı, oransal ağırlık artışı ve yem dönüşüm etkinlikleri 18°C de beslenen
balıklardan daha düşük bulunmuştur. Bu durum, 34°C su sıcaklığında yaşayan
doktor balıkların metabolizmalarının daha yüksek, daha hareketli ve terapiye
daha istekli olmalarından kaynaklanmış olabilir. İncelenen dokuların CAT
aktiviteleri ve MDA seviyelerinin önemli derecede farklı olduğu tespit
edilmiştir. En yüksek CAT aktiviteleri ve MDA seviyelerinin bağırsak
dokularında olduğu görülmüştür. Fakat 34°C su sıcaklığında beslenen balıkların
incelenen tüm dokularındaki CAT aktiviteleri ve MDA seviyelerinin 18°C su
sıcaklıklarında beslenen balıklardan önemli derecede farklı olmadığı tespit
edilmemiştir.
    

Kaynakça

  • [1] Ündar, L., Akpınar, M. A., Yanıkoğlu, A. 1990. Doctor fish and psoriasis. The Lancet, 335(1990), 470 - 471.
  • [2] Karahan A. 2007. Comparative investigation of Garra rufa and Garra variabilis by account of sitogenetic and morphometric. Mersin University, Institute of Science, Doctorate Thesis, 123s, Mersin.
  • [3] Sayili, M., Akça, H., Duman, T., Esengün, K. 2007. Psoriasis treatment via doctor fishes as part of healthtourism: A casestudy of Kangal Fish Spring, Turkey. Tourism Management, 28(2007), 625-629.
  • [4] Karaaslan M. 2010. The effects of different stress sources on antioxidant enzyme activities in fishes (Cyprinidae) from Sivas Kangal fish spring. Cumhuriyet University, Institute of Science, Master Thesis, 87s, Sivas.
  • [5] Bhat, M. K. 2000. Cellulases and Related Enzymes in Biotechnology. Biotechnology Advances, 18(2000), 355-383.
  • [6] Mawadza, C., Kaul, R. H., Zvauya, R., Mattiason, B. 2000. Purification and characterization of cellulases produced by two Bacillus strains. Journal of Biotecnology, 83(2000), 177-187.
  • [7] Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72(1976), 248.
  • [8] Draper, H. H., Hadley, M. 1990. Malondialdehyde determination as index of lipid peroxidation. Methods in Enzymology 180(1990), 421-431.
  • [9] Aebi, H. 1974. Catalase Methods of Enzymatic analysis, B. HU. New York and London, Academic, Press Inc (1974), 673-677.
  • [10] Akköse, F. 2012. The effects of different temperature on sexual differentation and development in sea bass (Dicentrarchus labrax L., 1758). Çukurova University, Institute of Science, Doctorate Thesis, 110s, Adana.
  • [11] Duman, S. 2010. Cyprinion macrostomus Heckel, 1843 and Garra rufa Heckel, 1843 species of fishes that is living in Kangal (Sivas) Balıklı Çermik Thermal Hot Spring with Topardıç Stream (Sivas) defines some histological parameters and non-specific immune respond. Çukurova University, Institute of Science, Doctorate Thesis, 89s, Adana.
  • [12] Bozkale, Ş. 2010. Isolation of Hsp70 under different stress factors from Sivas Kangal Hot Spring fish species (Cyprinidae). Cumhuriyet University, Institute of Science, Master Thesis, 89s,Sivas.

Effects of Water Temperature on Catalase Activity and Malondialdehyde Level in Muscle, Mouth, and Intestine Tissues of Doctor Fish (Garra rufa)

Yıl 2019, Cilt: 23 Sayı: 1, 191 - 194, 01.04.2019
https://doi.org/10.19113/sdufenbed.483082

Öz










In this study, the catalase
(CAT) activities and malondialdehyde (MDA) levels of some tissues (muscle,
mouth and intestine) of Garra rufa
(doctor fish) fed in different water temperatures (18°C and 34°C) were studied.
No fish death was observed during the experiments.
Despite the different significance levels, the proportional length
increase, the proportional weight increase, food conversion efficiency of
doctor fishes fed at
34°C were found to be lower than in fishes fed at 18°C. This can be due to the
fact that doctor fishes living in a water temperature of 34°C have a higher
metabolism, are more motile, and are more ready to the therapy. The CAT
activities and the MDA levels of the tissues studied were found to be
significantly different. The highest CAT activities and MDA levels were observed
in intestine tissues. But the CAT activities and the MDA levels of the tissues
studied for the fishes fed in 34°C water temperature were not significantly
different from those of fishes fed in
18°C water temperature.    

Kaynakça

  • [1] Ündar, L., Akpınar, M. A., Yanıkoğlu, A. 1990. Doctor fish and psoriasis. The Lancet, 335(1990), 470 - 471.
  • [2] Karahan A. 2007. Comparative investigation of Garra rufa and Garra variabilis by account of sitogenetic and morphometric. Mersin University, Institute of Science, Doctorate Thesis, 123s, Mersin.
  • [3] Sayili, M., Akça, H., Duman, T., Esengün, K. 2007. Psoriasis treatment via doctor fishes as part of healthtourism: A casestudy of Kangal Fish Spring, Turkey. Tourism Management, 28(2007), 625-629.
  • [4] Karaaslan M. 2010. The effects of different stress sources on antioxidant enzyme activities in fishes (Cyprinidae) from Sivas Kangal fish spring. Cumhuriyet University, Institute of Science, Master Thesis, 87s, Sivas.
  • [5] Bhat, M. K. 2000. Cellulases and Related Enzymes in Biotechnology. Biotechnology Advances, 18(2000), 355-383.
  • [6] Mawadza, C., Kaul, R. H., Zvauya, R., Mattiason, B. 2000. Purification and characterization of cellulases produced by two Bacillus strains. Journal of Biotecnology, 83(2000), 177-187.
  • [7] Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72(1976), 248.
  • [8] Draper, H. H., Hadley, M. 1990. Malondialdehyde determination as index of lipid peroxidation. Methods in Enzymology 180(1990), 421-431.
  • [9] Aebi, H. 1974. Catalase Methods of Enzymatic analysis, B. HU. New York and London, Academic, Press Inc (1974), 673-677.
  • [10] Akköse, F. 2012. The effects of different temperature on sexual differentation and development in sea bass (Dicentrarchus labrax L., 1758). Çukurova University, Institute of Science, Doctorate Thesis, 110s, Adana.
  • [11] Duman, S. 2010. Cyprinion macrostomus Heckel, 1843 and Garra rufa Heckel, 1843 species of fishes that is living in Kangal (Sivas) Balıklı Çermik Thermal Hot Spring with Topardıç Stream (Sivas) defines some histological parameters and non-specific immune respond. Çukurova University, Institute of Science, Doctorate Thesis, 89s, Adana.
  • [12] Bozkale, Ş. 2010. Isolation of Hsp70 under different stress factors from Sivas Kangal Hot Spring fish species (Cyprinidae). Cumhuriyet University, Institute of Science, Master Thesis, 89s,Sivas.
Toplam 12 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Mühendislik
Bölüm Makaleler
Yazarlar

Kazım Uysal 0000-0001-6399-6616

Yıldız Çolak Bu kişi benim 0000-0001-8753-5873

Mustafa Kavasoğlu 0000-0002-4136-6397

Yayımlanma Tarihi 1 Nisan 2019
Yayımlandığı Sayı Yıl 2019 Cilt: 23 Sayı: 1

Kaynak Göster

APA Uysal, K., Çolak, Y., & Kavasoğlu, M. (2019). Effects of Water Temperature on Catalase Activity and Malondialdehyde Level in Muscle, Mouth, and Intestine Tissues of Doctor Fish (Garra rufa). Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 23(1), 191-194. https://doi.org/10.19113/sdufenbed.483082
AMA Uysal K, Çolak Y, Kavasoğlu M. Effects of Water Temperature on Catalase Activity and Malondialdehyde Level in Muscle, Mouth, and Intestine Tissues of Doctor Fish (Garra rufa). SDÜ Fen Bil Enst Der. Nisan 2019;23(1):191-194. doi:10.19113/sdufenbed.483082
Chicago Uysal, Kazım, Yıldız Çolak, ve Mustafa Kavasoğlu. “Effects of Water Temperature on Catalase Activity and Malondialdehyde Level in Muscle, Mouth, and Intestine Tissues of Doctor Fish (Garra Rufa)”. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi 23, sy. 1 (Nisan 2019): 191-94. https://doi.org/10.19113/sdufenbed.483082.
EndNote Uysal K, Çolak Y, Kavasoğlu M (01 Nisan 2019) Effects of Water Temperature on Catalase Activity and Malondialdehyde Level in Muscle, Mouth, and Intestine Tissues of Doctor Fish (Garra rufa). Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi 23 1 191–194.
IEEE K. Uysal, Y. Çolak, ve M. Kavasoğlu, “Effects of Water Temperature on Catalase Activity and Malondialdehyde Level in Muscle, Mouth, and Intestine Tissues of Doctor Fish (Garra rufa)”, SDÜ Fen Bil Enst Der, c. 23, sy. 1, ss. 191–194, 2019, doi: 10.19113/sdufenbed.483082.
ISNAD Uysal, Kazım vd. “Effects of Water Temperature on Catalase Activity and Malondialdehyde Level in Muscle, Mouth, and Intestine Tissues of Doctor Fish (Garra Rufa)”. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi 23/1 (Nisan 2019), 191-194. https://doi.org/10.19113/sdufenbed.483082.
JAMA Uysal K, Çolak Y, Kavasoğlu M. Effects of Water Temperature on Catalase Activity and Malondialdehyde Level in Muscle, Mouth, and Intestine Tissues of Doctor Fish (Garra rufa). SDÜ Fen Bil Enst Der. 2019;23:191–194.
MLA Uysal, Kazım vd. “Effects of Water Temperature on Catalase Activity and Malondialdehyde Level in Muscle, Mouth, and Intestine Tissues of Doctor Fish (Garra Rufa)”. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi, c. 23, sy. 1, 2019, ss. 191-4, doi:10.19113/sdufenbed.483082.
Vancouver Uysal K, Çolak Y, Kavasoğlu M. Effects of Water Temperature on Catalase Activity and Malondialdehyde Level in Muscle, Mouth, and Intestine Tissues of Doctor Fish (Garra rufa). SDÜ Fen Bil Enst Der. 2019;23(1):191-4.

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