Evaluation of the mixture "MagSelDex" for neuroprotection against offspring brain injury in endotoxin-induced chorioamnionitis: A Preliminary Study

Yıl 2024, , 49 - 61, 22.04.2024

Halil Aşçı , Mehtap Savran , Sıla Gülbağ Pınar , Nasıf Fatih Karakuyu , Orhan İmeci , Mehmer Abdulkadir Sevük , Mekin Sezik , Özlem Özmen

https://doi.org/10.22312/sdusbed.1347232

Öz

Objective: Chorioamnionitis resulting from preterm labor leads to concurrent damage in both the placenta and fetal brain. This study aims to explore the impact of incorporating antioxidants and anti-inflammatory agents, specifically selenium (Sel) and dexpanthenol (Dex), into the standard magnesium (Mg) regimen, in mitigating this damage.

Materials and Methods: A total of six pregnant rats were assigned to six distinct groups: control, lipopolysaccharide (LPS) (1 mg/kg, single intraperitoneal dose on day 17), Mg (60 mg/kg Mg, intraperitoneal), Mg+Sel (1 mg/kg, intraperitoneal), Mg+Dex (500 mg/kg, intraperitoneal), and Mg+Sel+Dex. On the 17th day of pregnancy, fetal brain and placenta tissues were harvested for histopathological examination and immunohistochemical evaluation of tumor necrosis factor-alpha (TNF-α) and neurofilament expression.

Results: The histopathological assessment revealed LPS-induced hemorrhage and mild inflammatory cell infiltration in the placenta, and pronounced hyperemia along with minor hemorrhage in the fetal brain. The LPS group exhibited significantly elevated TNF-α expression in both placenta and fetal brain, coupled with reduced neurofilament expression in the fetal brain. In contrast, the groups treated with Mg alone and the combined Sel and Dex therapy exhibited moderate to substantial improvement in pathological findings across both tissues. The most notable enhancement was observed in the Mg+Sel+Dex group.

Conclusion: Administration of Mg as a standalone treatment and the coadministration of Sel and Dex effectively shielded the placenta and fetal brain from LPS-triggered chorioamnionitis. However, the most prominent protective effect was observed in the Mg+Sel+Dex group.

Anahtar Kelimeler

Brain injury, Corioamnionitis, Dexpanthenol, Magnesium, Selenium

Proje Numarası

TSG-2022-8783

Bakteriyel endotoksin kaynaklı koryoamniyonitte fetal beyin hasarına karşı "MagSelDex" karışımının nöroprotektif etkilerinin değerlendirilmesi: Ön Çalışma

Öz

Amaç: Preterm doğum kaynaklı koryoamnionitin plasenta ve fetal beyinde eş zamanlı hasara neden olduğu bilinmektedir. Bu çalışma, standart magnezyum (Mg) rejimine antioksidan ve anti-inflamatuar ajanlar olan selenyum (Sel) ve dekspantenol (Dex) eklemenin, bu hasarı hafifletmedeki etkisini araştırmayı amaçlamaktadır.
Gereç ve Yöntem: Toplam altı gebe sıçan, altı ayrı gruba ayrılmıştır: kontrol, lipopolisakkarit (LPS) (1 mg/kg, 17. gün tek doz intraperitoneal uygulama), Mg (60 mg/kg, intraperitoneal), Mg+Sel (1 mg/kg, intraperitoneal), Mg+Dex (500 mg/kg, intraperitoneal) ve Mg+Sel+Dex. Gebeliğin 17. gününde fetal beyin ve plasenta dokuları histopatolojik inceleme ile tümör nekroz faktör-alfa (TNF-α) ve nörofilament (NF) ekspresyonlarının immünohistokimyasal değerlendirmesi için toplandı.
Bulgular: Histopatolojik değerlendirme, plasentada LPS kaynaklı kanama ve hafif inflamatuar hücre infiltrasyonunu ortaya çıkardı; aynı zamanda fetal beyinde belirgin hiperemi ve hafif kanama görüldü. LPS grubunda, hem plasenta hem de fetal beyinde belirgin şekilde yükselmiş TNF-α ekspresyonu ve fetal beyinde azalmış NF ekspresyonu gözlendi. Bunun aksine, yalnızca Mg ile tedavi edilen gruplar ve birlikte uygulanan Sel ve Dex tedavisi, her iki dokuda da patolojik bulgularda düzelme sergiledi. En dikkate değer iyileşme Mg+Sel+Dex grubunda gözlendi.
Sonuç: Mg’ nin tek başına uygulanması ve Sel ile Dex' in birlikte uygulanması, plasentayı ve fetal beyini LPS ile indüklenmiş koryoamniyonitten etkili bir şekilde korudu. Ancak, en belirgin koruyucu etki Mg+Sel+Dex grubunda gözlemlendi.

Anahtar Kelimeler

Beyin hasarı, Koryoamniyonit, Dekspantenol, Magnezyum, Selenyum

Destekleyen Kurum

Süleyman Demirel Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi

Proje Numarası

TSG-2022-8783

Kaynakça

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