Gümüş Nanopartiküllerin Biberiye Özütü ile Biyosentezi ve MCF-7 Meme Kanseri Hücrelerinde Sitotoksik Etkisi

Öz

Amaç: Gümüş nanopartiküller (AgNP'ler), antimikrobiyal ve dezenfektan maddeler olarak uzun yıllar kullanılmıştır. Bununla birlikte, gümüş nanopartiküllerin antikanser potansiyeli hakkında oldukça sınırlı bilgi mevcuttur. Bu nedenle, bu çalışmada AgNP’lerin MCF7 meme kanseri hücreleri üzerindeki sitotoksik etkisinin değerlendirilmesi amaçlanmıştır. 

Materyal-Metot: Biberiye (Rosmarinus officinalis) özütü kullanılarak gümüş nanopartiküllerin sentezi için bir biyosentez metodu geliştirildi. Sentezlenmiş gümüş nanopartiküllerin UV-Visible Spektrofotometre ile karakterizasyonu yapıldı. MCF7 meme kanseri hücreleri çeşitli konsantrasyonlarda AgNP’ler (0-10 μg/mL) ile 24 saat boyunca muamele edildi.  Gümüş nanopartiküllerin sitotoksik etkisi MTT test ile belirlendi. 

Bulgular: AgNP’lerin MTT testi kullanılarak doza bağımlı hücre büyümesini inhibe ettiği belirlendi. 

Sonuç: Bu çalışma ile bitki özütü kullanılarak nanopartiküllerin sentezinin fiziksel ve kimyasal yöntemlere göre daha iyi bir alternatif olduğu ve AgNP’lerin meme kanseri tedavisinde potansiyel alternatif bir ajan olabileceğini sonucuna varılmıştır.  

Anahtar Kelimeler

• Gümüş Nanopartiküller,MCF-7,Rosmarinus officinalis,Biberiye

Kaynakça

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