Increased Systemic Oxidative Stress and DNA Damage in Patients with Exudative Age-Related Macular Degeneration

Yıl 2019, Cilt: 10 Sayı: 3, 273 - 277, 20.09.2019

Emine Kılıç Toprak , İbrahim Toprak , Volkan Yaylalı Yasin Özdemir Burak Oymak Melek Bor Küçükatay , Vural Küçükatay

https://doi.org/10.22312/sdusbed.553227

Öz

Amaç: Bu çalışmanın
amacı eksudatif yaşa bağlı makula dejenerasyonu (YBMD) olan hastalarda sistemik
oksidatif stres ve genotoksisite (DNA hasarı) bulgularını araştırmaktır.

Materyal-metod:
Çalışmaya eksudatif YBMD'li 26 hasta (68.9±1.9 yaş) ve 26 yaş ve cinsiyet
uyumlu (64.8±2.2 yaş) sağlıklı kontrol grubu (p=0.268, p=0.258) alındı. Serum total
antioksidan durumu (TAS) ve total oksidan durumu (TOS) ticari bir kit
kullanılarak ölçüldü. Oksidatif stres indeksi (OSİ) hesaplandı. DNA hasarı,
venöz tam kandan lenfosit izolasyonunu takiben Comet (kuyruklu yıldız) analizi
kullanılarak değerlendirildi. Kuyruk uzunluğu (TL) ve kuyruk momenti (TM) dahil
olmak üzere parametreler, DNA hasarının kantitatif analizi için
değerlendirildi.

Bulgular: YBMD grubunda
kontrol grubuna göre daha düşük TAS ve daha yüksek OSİ değerleri mevcut iken,
TOS değerleri iki grup arasında farklılık göstermedi (p=0.006, p=0.039, p=0.319,
sırasıyla). Ayrıca, YBMD grubunda TL (p<0.0001) ve TM (p=0.005) kontrol
grubuna göre anlamlı olarak yüksek bulundu.

Sonuç: Eksudatif YBMD
hastalarında sistemik oksidatif stres indeksinin ve DNA hasarının daha yüksek
olduğu görülmektedir.

Anahtar Kelimeler

Comet analizi, DNA hasarı, maküler dejenerasyon

Systemic Oxidant and Genotoxic Background in Exudative Age-Related Macular Degeneration

Öz

Objective: The purpose of this study was to investigate the
evidence of systemic oxidative stress and genotoxicity (DNA damage) in patients
with exudative age-related macular degeneration (AMD).

Material
-Method: The study comprised
26 patients with exudative AMD (68.9±1.9 years of age) and 26 age- and
sex-matched (64.8±2.2 years of age) healthy controls (p=0.268, p=0.258,
respectively). Serum total
antioxidant status (TAS) and total oxidant status (TOS) were measured with a
commercial kit. Oxidative stress index (OSI) was calculated. DNA damage was evaluated
using comet analysis following lymphocyte isolation from venous blood.
Parameters including tail length (TL) and tail moment (TM) were evaluated for
quantitative analysis of DNA damage.

Results:
The AMD group had lower TAS and
higher OSI values than in the control group, while TOS values did not differ
between the two groups (p=0.006, p=0.039, p=0.319, respectively). Furthermore,
TL (p<0.0001) and TM (p=0.005) were significantly higher in the AMD group
compared to the control group.

Conclusion:
Systemic oxidative stress index and
cumulative DNA damage appear to be increased in patients with exudative AMD. 

Anahtar Kelimeler

Comet assay, DNA damage, macular degeneration

Kaynakça

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