IN SILICO ANALYSIS OF THE POTENTIAL ROLE OF SRY GENE IN PARKINSON’S DISEASE

Öz

Objective Parkinson’s disease (PD) is the most common movement disorder and is caused by the loss of dopaminergic neurons in the substantia nigra. The first motor symptoms of PD begin to appear when more than 60% of dopaminergic neurons are already lost. Although the etiology of PD is not fully understood, PD is believed to result from the complex interactions between genetic and environmental factors. Previous studies demonstrated that men are at least 1.5 times more likely to develop PD than women. The current kulstudy aimed to investigate whether SRY, a chromosome gene, plays a role in the pathogenesis of PD using in silico methods. Material and Method The mRNA expression of SRY in different human tissues was analyzed using the Human Protein Atlas (HPA). The interactions between SRY and PD-related genes were analyzed using the STRING and GeneMANIA databases. The JASPAR database was used to determine the putative binding sites of SRY in the promoter regions of some PD-related genes. Finally, the BioGRID and GeneMANIA databases were used to identify the key genes that interact with SRY. Results In silico analyses revealed that there are SRY binding sites in the promoter regions of some PD-associated genes and that SRY can regulate androgen receptor (AR) signaling. Conclusion These findings suggest that SRY may play a role in the pathogenesis of PD and may thus serve as a molecular target for developing new therapeutic strategies against PD.

Anahtar Kelimeler

• Parkinson’s disease
• SRY
• expression
• promoter
• in silico

SRY GENİNİN PARKİNSON HASTALIĞINDAKİ POTANSİYEL ROLÜNÜN İN SİLİKO ANALİZİ

Öz

Amaç Parkinson hastalığı (PH) en sık görülen hareket hastalığıdır ve substansiya nigra bölgesindeki dopaminerjik nöronların kaybından kaynaklanmaktadır. PH’nin ilk motor semptomları ortaya çıkmaya başladığında dopaminerjik nöronların %60’ından fazlası zaten ölmüştür. PH’nin etiyolojisi tam olarak anlaşılmamış olsa da, PH’nin genetik ve çevresel faktörler arasındaki kompleks etkileşimler sonucu oluştuğuna inanılmaktadır. Önceki çalışmalar erkeklerin PH’ye yakalanma olasılığının kadınlara göre en az 1,5 kat daha fazla olduğunu göstermiştir. Bu çalışma, bir Y kromozomu geni olan SRY’nin PH’nin patogenezinde bir rol oynayıp oynamadığını in siliko yöntemlerle araştırmayı amaçlamıştır. Gereç ve Yöntem Farklı insan dokularındaki SRY mRNA ekspresyonu İnsan Protein Atlas (HPA)’yı kullanarak analiz edilmiştir. SRY ve PH ile ilişkili genler arasındaki etkileşimler STRING ve GeneMANIA veri tabanlarını kullanarak incelenmiştir. JASPAR veri tabanı, PH ile ilişkili bazı genlerin promotör bölgelerindeki muhtemel SRY bağlanma noktalarını belirlemek için kullanılmıştır. Son olarak, SRY ile etkileşim halindeki anahtar genleri belirlemek için BioGRID ve GeneMANIA veri tabanları kullanılmıştır. Bulgular İn siliko analizler PH ile ilişkili bazı genlerin promotör bölgelerinde SRY bağlanma noktası olduğunu ve SRY’nin androjen reseptör (AR) sinyalini düzenleyebileceğini ortaya çıkarmıştır. Sonuç Bu bulgular SRY’nin PH’nin patogenezinde rol oynayabileceğini ve bundan dolayı da PH’ye karşı yeni terapötik stratejiler geliştirmek için moleküler bir hedef vazifesi görebileceğini önermektedir.

Anahtar Kelimeler

• Parkinson hastalığı
• SRY
• ekspresyon
• promotör
• in siliko

Kaynakça

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