SİNİR BÜYÜME FAKTÖRÜ İLE FARKLILAŞTIRILMIŞ PC12 HÜCRELERİNİN MORFOMETRİK VE FLORESANS ANALİZİ

Year 2023, , 732 - 737, 30.12.2023

Simge Ünay , Ferhat Şirinyıldız

https://doi.org/10.17343/sdutfd.1329845

Abstract

Amaç
PC12 bir sıçan feokromositoma hücre hattıdır. Bu
hücreler, sinir büyüme faktörü (NGF) ile kültürlendiğinde
karakteristik olarak farklılaşmaya uğramaktadır.
NGF dozuna bağlı olarak, nörit uzantılarının uzunlukları
değişir. Bu farklılaşma özelliği sayesinde hücreler
nörobilimde ve Alzheimer, Parkinson, Amyotrofik Lateral
Skleroz gibi patofizyolojik hastalıkların modellenmesinde
kullanılmaktadır. Ancak NGF’nin PC12
hücrelerinde oluşan nörit uzantıları üzerine olan etkisini
gösteren literatür çalışmaları oldukça kısıtlıdır. Bu
çalışmanın amacı NGF’nin doza ve inkübasyon süresine
bağlı olarak nörit uzantıları ve hücre canlılığı
üzerine olan etkisini araştırmaktır.
Gereç ve Yöntem
Bu çalışmada PC12 hücreleri 50 ng/ml ve 100 ng/ml
NGF ile 3, 6 ve 7 gün inkübe edilmiştir. İnkübe edilen
hücrelerde nörit büyümelerinin uzunlukları ve ölü hücre
oranları hesaplanmıştır.
Bulgular
Elde edilen bulgular ile NGF dozlarına ve inkübasyon
süresine bağlı olarak nörit uzantılarının uzunluğunun
ve ölü hücre oranının arttığı gösterilmiştir. NGF inkübasyon
süreleri karşılaştırıldığında 50 ng/ml NGF 6
gün ve 100 ng/ml NGF 3 gün grupları arasında fark
olmadığı bulunmuştur.
Sonuç
Deney gruplarında ölü hücre oranları ve nörit uzantılarının
boyutları değerlendirildiğinde 100 ng/ml NGF
ve 3 gün inkübasyon süresi parametrelerinin PC12
hücre farklılaşması için ideal olduğu düşünülmektedir.

Keywords

Nörit uzunluğu, PC12 hücre hattı, Sinir büyüme faktörü

Project Number

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MORPHOMETRIC AND FLUORESCENCE ANALYSIS OF NERVE GROWTH FACTOR-DIFFERENTIATED PC12 CELLS

Abstract

Objective
PC12 is a rat pheochromocytoma cell line. These
cells characteristically undergo differentiation when
cultured with nerve growth factor (NGF). Depending
on the dose of NGF, the length of neurite extensions
changes. Thanks to this differentiation property,
the cells are used in neuroscience and in modeling
pathophysiological diseases such as Alzheimer's,
Parkinson's, and Amyotrophic Lateral Sclerosis.
However, literature studies showing the effect of NGF
on neurite extensions formed in PC12 cells are very
limited. This study aimed to investigate the effect of
NGF on neurite extensions and cell viability depending
on dose and incubation time.
Materials and Methods
In this study, PC12 cells were incubated with 50 ng/ml
and 100 ng/ml NGF for 3, 6 and 7 days. The lengths
of neurite outgrowths and dead cell ratios were
calculated in incubated cells.
Results
The results showed that the length of neurite
extensions and dead cell ratio increased depending
on NGF doses and incubation time. When NGF
incubation times were compared, no difference was
found between 50 ng/ml NGF 6 days and 100 ng/ml
NGF 3 days groups.
Conclusion
When the dead cell ratios and sizes of neurite
extensions in the experimental groups are evaluated,
it is thought that 100 ng/ml NGF and 3 days incubation
time parameters are ideal for PC12 cell differentiation.

Keywords

Nerve growth factor, Neurite length, PC12 cell line

Supporting Institution

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Project Number

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Thanks

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