Uterin leyomiyomlarda vasküler endotelyal büyüme faktörü ve matriks metalloproteinaz-2 ekspresyonu ve anjiyogenezle ilişkisi

Öz

Amaç: Leyomiyom uterusun en sýk görülen tümörüdür. Uterin leyomiyomun büyümesinde anjiyogenik faktörler ve proteazlar rol oynayabilir. Çalýþmamýzýn amacý uterin leyomiyomlarda vasküler endotelyal büyüme faktörü (VEGF) ve matriks metalloproteinaz-2 (MMP-2) ekspresyonunu araþtýrmak ve mikrodamar yoðunluðu (MVD) ile olasý iliþkilerini belirlemekti. Gereç ve yöntemler: Çalýþma materyali leyomiyom nedeni ile opere edilmiþ 50 hastanýn leyomiyom ve normal miyometriyumunu içeren parafin bloklardan oluþuyordu. VEGF, MMP-2 ve CD34 ekspresyonu immünohistokimyasal olarak çalýþýldý. VEGF ve MMP-2 ye ait immünohistokimyasal yöntemle elde edilen bulgular semikantitatif analiz ile deðerlendirildi. MVD ise, CD34 pozitif vasküler endotelyal hücreleri sayarak hesaplandý. Bulgular: Leyomiyomlardaki VEGF ve MMP-2 ekspresyonu normal miyometriyuma göre anlamlý olarak daha yüksek bulundu (p=0.001). CD34 boyamasý MVD nun leyomiyomlarda miyometriyuma göre daha az olduðunu gösterdi (p<0.05). VEGF ve MMP-2 boyanmasý ile MVD arasýnda korelasyon saptanmadý. Sonuç: Bu bulgular uterin leyomiyomlarýn patogenezinde VEGF ve MMP-2 nin rol oynayabileceðini düþündürmüþtür. Bu hastalýðýn geliþim sürecine anjiyogenez dýþýndaki bazý faktörler de katkýda bulunabilir.

Anahtar Kelimeler

• Uterus
• leyomiyom
• VEGF
• MMP-2
• mikrodamar yoðunluðu

Expression of vascular endothelial growth factor and matrix metalloproteinase-2 in uterine leiomyoma and correlation with angiogenesis

Öz

Objective: Leiomyoma is the most common tumor of the uterus. Angiogenic factors and proteases may play role in the growth of uterine leiomyoma.The aim of our study was to investigate the expression of vascular endothelial growth factor (VEGF) and matrix metalloproteinase-2 (MMP-2) in uterine leiomyoma and their possible relation to microvessel density (MVD). Material and methods: Material for the study comprised parafin-embedded tissue sections of uterine leiomyomas and corresponding myometrium derived from 50 hysterectomized women. VEGF, MMP-2 and CD34 expression was investigated immunohistochemically. Semiquantitative analysis of the immunostainings for VEGF and MMP-2 was performed. MVD was calculated by counting of CD34 positive vascular endothelial cells. Results: Expression of VEGF and MMP-2 was significantly higher in leiomyomas than corresponding myometrium (p=0.001). The CD34 labeling showed decreased MVD in leiomyomas compared with myometrium (p<0.05). There was no correlation between VEGF and MMP-2 staining and MVD. Conclusion: These data suggested that VEGF and MMP-2 may play role in the development of uterine leiomyomas. Mechanisms other than promotion of angiogenesis may contribute to this disease process.

Anahtar Kelimeler

• Uterus
• leiomyoma
• VEGF
• MMP-2
• microvessel density

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