The Effectivity of Centella Asiatica Extract in Experimental Colitis Model Induced by Dextran Sulfate Sodium in Rats

Öz

Background: Inflammatory bowel disease (IBD) is a group of chronic and inflammatory conditions that are considered to be caused by environmental, genetic, and immune factors. The treatment of IBD mainly involves combinations of anti-inflammatory, immunosuppressive, and cytotoxic agents. However, due to serious side effects of these agents, research is being performed to find new treatment methods.

Methods:The 24 rats were divided into three groups. In control group, the rats received drinking water and standard rat chow, in colitis group, the rats received drinking water with DSS, and in CA+colitis group the rats were administered 200 mg/kg/day CA and then received drinking water with DSS. Histopathologic examination, concentrations of myeloperoxidase (MPO),  malondialdehyde (MDA), tumor necrosis factor alpha (TNF-α), and interleukin-10 (IL-10), were determined.

Results: The results indicated that the TNF-α level was lower and the IL-10 was higher in the rats administered with CA extract compared to the rats in the colitis group (p<0.05). The MPO and MDA levels were significantly higher in the colitis group compared to other groups (p<0.05). Histopathologic examination revealed less tissue damage in the rats administered with CA extract.

Conclusion: The CA extract exerts an immunoregulatory effect by altering the levels of anti- and pro-inflammatory cytokines in experimental IBD models. It also prevents tissue damage by inhibiting the formation of free oxygen radicals. Depending on these effects, we consider that the CA extract can be a useful agent in the treatment of IBD.

Anahtar Kelimeler

• Inflammatory bowel disease,Centella asiatica,Dextran sulfate sodium,Eksperimental colitis

Deneysel Kolit Modelinde Centella Asiatika Ekstresinin Etkinliği

Öz

Giriş: İnflamatuvar barsak hastalıkları (İBD) çevresel, genetik, immun faktörlerin sebep olduğu düşünülen bir grup kronik ve inflamatuvar durum olarak tanımlanmaktadır. Tedavisinde basilica antiinflamatuvar, immünsupresif ve sitotoksik ilaç kombinasyonları kullanılmaktadır. Fakat bu ajanların ciddi yan etkilerinden dolayı yeni tedavi yöntemleri bulmak için araştırmalar yapılmaktadır. Bu çalışmanın amacı dekstran sülfat sodium (DSS) indüklenmiş deneysel kolit modelinde Centella Asiatica (CA) ekstresinin etkinliğini araştırmaktır.

Metod: Çalışmada 24 adet Wistar Albino rat üç eşit gruba ayrıldı. Kontrol grubuna normal içme suyu ve standart rat yemi verildi. Kolit grubuna DSS’ li içme suyu ve CA+kolit grubuna ise 200mg/kg/gün CA ekstresi ve DSS’ li içme suyu verildi. Deney sonunda histopatolojik inceleme ve myeloperoksidaz (MPO), malonildialdehit (MDO), tümor nekroz factör alfa (TNF-α) ve interlökin-10 (IL-10) düzeyleri belirlendi.  

Bulgular: TNF- α, MPO ve MDA düzeyleri kolit grubunda anlamlı düzeyde daha yüksek bulundu. İL- 10 düzeyi ise CA+kolit grubunda daha yüksekti (p<0.05). Histopatolojik incelemede  CA+kolit grubunda daha az doku hasarı saptandı.

Sonuç: CA ekstresi proinflamatuvar ve antiinflamatuvar sitokin düzeyini değiştirerek immünregulatuvar bir etki göstermektedir. Ayrıca serbest oksijen radikalleri oluşumunu önleyerek doku hasarını önlemektedir. Bu etkilerinden dolayı CA ekstresinin IBD tedavisinde faydalı bir ajan olarak kullanılabileceğini düşünmekteyiz. 

Anahtar Kelimeler

• İnflamatuvar barsak hastalıkları,Centella Asiatika,Dekstran sülfat sodium,Deneysel kolit

Kaynakça

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