In vitro cytotoxic evaluation of conventional denture base material and soft lining material using colorimetric MTT assay

Year 2018, , 157 - 166, 01.05.2018

Canan Akay , Merve Çakırbay Tanış

https://doi.org/10.17343/sdutfd.337797

Abstract

Abstract

Purpose:
In this study, it was aimed to evaluate the time course of cytotoxic effects of
the conventional base material and soft lining material on the mouse fibroblast
cells.

Material
and Method:

Disc-shaped
test samples of denture base material (rodex) and soft lining material(
dentusil) were fabricated according to manufacturers' instructions under
aseptic conditions. The samples were subjected to 5,000
thermal cycling to mimic the oral environment. Following aging procedures, the
cytotoxic effect of the materials was assessed by [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium
bromide]  assay using L929 mouse
fibroblast cells after 24 h, 48 h and 72 h cell incubation period. Cell
viability values were calculated for each group. Statistical analysis of the
data was performed using a two-way repeated measurement method. (p<0,001)

Results:
For 24
hours and 48 hours incubation period soft lining material, and  for 72 hour incubation period the base
material showed more cell viability. Statistically, there was a significant
difference between the two materials. During the incubation period, the group
incubated 24 hours is statistically different from 48 hours and 72 hours. No
significant difference was found between 72 hours and 48 hours.

Conclusion: The soft lining
material we used under the base materials is more biocompatible than the base
material.

Keywords

Conventional denture base material, soft liners, cytotoxicity, thermal cycles

Kolorimetrik MTT testi kullanarak geleneksel protez kaide materyali ile yumuşak astar materyalinin in vitro sitotoksik özelliklerinin değerlendirilmesi

Abstract

Özet

Amaç:
Bu çalışmada protez yapımında kullanılan geleneksel kaide materyali ile yumuşak
astar materyalinin fare fibroblast hücreleri üzerinde zamanla meydana gelen
sitotoksik etkilerinin değerlendirilmesi amaçlandı.

Materyal
ve Metod: Protez kaide materyali (rodeks) ve yumuşak astar materyalinin (dentusil)
disk şekilli test numuneleri üreticinin talimatlarına göre aseptik şartlar
altında hazırlandı.. Örnekler, ağız ortamını taklit etmek için 5.000 termal
döngüye tabi tutuldu. Yaşlanma prosedürlerini takiben, materyallerin sitotoksik
etkisi, 24 saat, 48 saat ve 72 saatlik hücre inkubasyon döneminden sonra L929
fare fibroblast hücreleri kullanılarak [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium
bromide] testi ile değerlendirildi. Her grup için hücre canlılığı değerleri
hesaplandı. Verilerin istatistiksel analizi, iki yönlü tekrarlanan bir ölçüm
yöntemi kullanılarak gerçekleştirildi. (P <0.001)

Bulgular: 24 saat ve 48 saat
inkubasyon periyodunda yumuşak astar materyali, 72 saat inkübasyon periyodunda
ise kaide materyali daha fazla hücre canlılığı göstermiştir. İstatistiksel
olarak iki materyal arasında anlamlı fark bulunmuştur. İnkübasyon periyotları arasında
ise 24 saat inkübe edilen grup 48 saat ve 72 saatden istatistiksel olarak
farklıdır. 72 saat ve 48 saat arasında anlamlı fark bulunamamıştır.

Sonuç: Kaide materyallerinin
altına kullanmış olduğumuz yumuşak astar materyali kaide materyaline göre daha
biyouyumludur.

Keywords

Geleneksel kaide materyali, yumuşak astar, sitotoksisite, termal siklus

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