DOES EXCESSIVE CONSUMPTION OF FLAXSEED (LINUM USITATISSIMUM L.) CAUSE A LIVER DAMAGE IN RAT MODELS?

Öz

Natural health products and functional foods are frequently consumed for the prevention and treatment of many diseases. Flaxseed is one of the functional foods with anticancer, antiviral, antibacterial and antifungal properties. In addition to its beneficial effects, excessive consumption without considering the appropriate dosage can cause toxic effects. In this study, it was planned to investigate the effects of different amounts of flaxseed consumption on rat liver. Materials and Methods Rats were randomly divided into four groups with 8 rats in each groups; control group and experimental groups which given flaxseed for 7 days; 1.4, 2.8 and 5.6 g/kg/day, respectively. At the end of the experiment, histological, immunohistochemical and biochemical analyzes were performed on tissue and blood samples. Results Serum AST, ALT and ALP values are significantly higher in 5.6 g/kg of flaxseed compared to control group (p values 0.003, 0.012 and 0.009, respectively). Albumin values were significantly lower in experimental groups compared to the control group (p<0.05). TOS and OSI increased significantly (p<0.05), TAS decreased (p>0.05) in all experimental groups compared to control. In histopathological findings, there was significant difference in three groups compared to control group, especially in 5.6 g/kg of flaxseed group compared to others. As a result of immunohistochemical analyzes, staining intensity of the receptors was highest in 5.6 g/kg of flaxseed group. The highest positive staining was observed respectively in NOX4, iNOS, TNF-α and IL-6. Conclusion These results show that excessive consumption of flaxseed can cause oxidative stress and toxicity due to inflammation in the liver and this study can helpful other studies about flaxseed toxicity.

Anahtar Kelimeler

• Flaxseed toxicity
• Liver Toxicity
• Rat

AŞIRI DOZ KETEN TOHUMU (LINUM USITATISSIMUM L.) TÜKETİMİ RATLARDA KARACİĞER HASARINA NEDEN OLUR MU?

Öz

Doğal sağlık ürünleri ve fonksiyonel gıdalar, birçok hastalığın önlenmesi ve tedavisi için tüketilmektedir. Keten tohumu antikanser, antiviral, antibakteriyel ve antifungal özelliklere sahip fonksiyonel gıdalardan biridir. Yararlı etkilerinin yanı sıra bilinçsiz ve aşırı tüketilmesi toksik etkilere neden olabilmektedir. Bu çalışmada, farklı miktarlarda keten tohumu tüketiminin sıçan karaciğeri üzerindeki etkilerinin araştırılması planlanmıştır. Gereç ve Yöntem Sıçanlar; her grupta 8 adet olarak; kontrol grubu ve 7 gün süreyle sırasıyla; 1.4, 2.8 ve 5.6 g/kg/gün keten tohumu verilen deney grupları şeklinde dört gruba ayrılmıştır. Deney sonunda doku ve kan örneklerinde histolojik, immünohistokimyasal ve biyokimyasal analizler yapılmıştır. Bulgular Serum AST, ALT ve ALP değerleri 5,6 g/kg keten tohumu verilen grupta kontrol grubuna göre anlamlı olarak daha yüksek gözlendi (p değerleri 0.003, 0.012 ve 0.009). Albümin değerleri deney gruplarında kontrol grubuna göre anlamlı olarak düşük gözlendi (p<0.05). TOS ve OSI kontrole göre tüm deney gruplarında anlamlı olarak artmış (p<0.05), TAS ise azalmıştı (p>0.05). Kontrol grubu ile deney grupları arasında histopatolojik bulgular arasında anlamlı farklar bulundu. Özellikle 5.6 g/kg keten tohumu grubunda tüm gruplara göre daha fazla histopatolojik bulgulara rastlandı. İmmünohistokimyasal analizlerde, en fazla boyama yoğunluğu 5,6 g/kg keten tohumu grubunda gözlemlendi. En yüksek pozitif boyanmaya sırasıyla NOX4, iNOS, TNF-α ve IL-6 boyanmalarında rastlandı. Sonuç Aşırı keten tohumu tüketiminin oksidatif stres ve toksisiteye bağlı olarak karaciğerde iltihaplanmaya neden olabileceği ve çalışmanın keten tohumu toksisitesi ile ilgili diğer çalışmalara katkı sağlayacağı düşünülmektedir.

Anahtar Kelimeler

• Keten tohumu toksisitesi
• Karaciğer toksisitesi
• Sıçan

Kaynakça

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