Batı Akdeniz Bölgesi Domates Güvesi Tuta absoluta (Lepidoptera: Gelechiidae) Popülasyonlarında Endosimbiyont Bakteri Türlerinin Belirlenmesi

Yıl 2023, , 1 - 4, 13.06.2023

Merve Kavak , Erhan Koçak

https://doi.org/10.54975/isubuzfd.1149112

Öz

Çalışma kapsamınsa, Batı Akdeniz Bölgesi’nde Antalya, Muğla, Isparta ve Burdur illerinden 11 lokasyondan toplam 55 adet Domates güvesi Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) bireyi toplanmıştır. Söz konusu böcek türünde endosimbiyont bakterilerden Wolbachia, Rickettsia ve Spiroplasma taraması yapılmıştır. Bu bireylerde endosimbiyont bakteri olan Wolbachia, Rickettsia ve Spiroplasma’nın varlığı ve bulunma oranı PCR reaksiyonlarından sekans yapılarak belirlenmiştir. Sonuç olarak, Rickettsia ve Spiroplasma’nın T. absoluta için dünya ve Türkiye genelinde ilk kayıt olduğu belirlenmiştir. Bu zamana kadar bulunamayan Spiroplasma ve Rickettsia çalışmamızda yüksek oranda değer göstermiştir. Batı Akdeniz Bölgesi’nde Domates güvesinde Wolbachia, Ricketsia ve Spiroplasma bulunma oranları sırasıyla %76.3, %78.1 ve %90.9 olarak belirlenmiştir.

Anahtar Kelimeler

Tuta absoluta, Endosimbiyont bakteri, Wolbachia, Ricketsia, Spiroplasma

Destekleyen Kurum

ISUBÜ Bap

Proje Numarası

2021 YL1-0124

Teşekkür

2021-YL1-0124 No’lu Proje kapsamında tezi maddi olarak destekleyen Isparta Uygulamalı Bilimler Üniversitesi Bilimsel Araştırma Projeleri Yönetim Birimi Başkanlığı’na teşekkür ederiz.

Determination of Endosymbiont Bacteria Species of Tomato Leafminer Tuta absoluta (Lepidoptera: Gelechiidae) Populations from the West Mediterranean Region

Öz

Within the scope of the study, a total of 55 tomato leafminer Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) individuals were collected from 11 locations in the provinces of Antalya, Muğla, Isparta and Burdur in the Western Mediterranean Region. Wolbachia, Rickettsia and Spiroplasma from endosymbiont bacteria were screened in the insect species in question. The presence and rate of endosymbiont bacteria Wolbachia, Rickettsia and Spiroplasma in these individuals were determined by sequencing from PCR reactions. As a result, it was determined that Rickettsia and Spiroplasma were the first records for T. absoluta in the world and Turkey. Spiroplasma and Rickettsia, which could not be found until now, showed high value in our study. In the region, the presence ratios of Wolbachia, Ricketsia and Spiroplasma were determined as 76.3%, 78.1% and 90.9%, respectively.

Anahtar Kelimeler

Tuta absoluta, Endosymbiont bacteria, Wolbachia, Ricketsia, Spiroplasma

Proje Numarası

2021 YL1-0124

Kaynakça

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