Cross-Resistance Relationship between Spiromesifen and Tebufenpyrad in Tetranychus urticae Koch (Acari:Tetranychidae) Populations Collected from Bean Fields in Burdur Province

Abstract

In this study, spiromesifen and tebufenpyrad cross-resistance was investigated in Tetranychus urticae Koch (Acari: Tetranychidae) populations collected from Burdur province during the 2023 bean production season. In Burdur province, where beans are produced intensively, 5 Tetranychus urticae populations were collected, 2 from the Central district, 2 from Çeltikçi district and 1 from Çavdır district. The dry residue method was used to determine the resistance levels of the populations. 7 insecticide doses + 1 control group were used in the trials. Each group consisted of 3 repetitions. There are 25 adult two spotted spider mite individuals in each replicate. Acaricides were applied to the leaf surface as 2ml at 1 atm pressure using a spraying tower. Dead and living counts in the populations were made after 24 hours. Spiromesifen and tebufenpyrad resistance ratios in Tetranychus urticae populations were determined by ratioing the LC50 values of the field populations to the LC50 value of the susceptible population.As a result of the study, spiromesifen resistance ratios of B1, B2, B3, B4 and B5 populations collected from bean fields of Tetranychus urticae were 98.1, 51.5, 177.1, 130.6 and 53.6 folds, respectively; tebufenpyrad resistance ratios were determined as 2.3, 1.1, 1.8, 1.8 and 1.0 folds. Cross-resistance between spiromesifen and tebufenpyrad has not been identified in two spotted spider mite populations. When the results are evaluated together, it is thought that spiromesifen should not be used in the control against Tetranychus urticae in these areas, and the use of tebufenpyrad would be more accurate.

Keywords

• Cross resistance
• Tetranychus urticae
• Tebufenpyrad
• Spiromesifen

Burdur İli Fasulye Alanlarından Toplanan Tetranychus urticae Koch (Acari:Tetranychidae) Popülasyonlarında Spiromesifen ve Tebufenpyrad Çapraz Direnç ilişkisi

Öz

Bu çalışmada, 2023 yılı fasulye üretim sezonu boyunca Burdur ilinden toplanan Tetranychus urticae Koch (Acari: Tetranychidae) popülasyonlarında spiromesifen ve tebufenpyrad çapraz direnci araştırılmıştır. Burdur ilinde yoğun olarak fasulye üretimi yapılan Merkez ilçesinden 2, Çeltikçi ilçesinden 2 ve Çavdır ilçesinden 1 olmak üzere 5 adet Tetranychus urticae popülasyonu toplanmıştır. Popülasyonların direnç düzeylerinin belirlenmesi için kuru rezidü yöntemi kullanılmıştır. Denemelerde 7 insektisit dozu+1 kontrol grubu kullanılmıştır. Her grup 3 tekerrürden oluşmuştur. Her tekerrürde ise 25 adet ergin kırmızıörümcek bireyi bulunmaktadır. Akarisitler ilaçlama kulesi kullanılarak 1 atm başınçta yaprak yüzeyine 2ml olarak uygulanmıştır. Popülasyonlarda ölü-canlı sayımları 24 saat sonra yapılmıştır. Tetranychus urticae popülasyonlarında spiromesifen ve tebufenpyrad direnç oranları tarla popülasyonlarının LC50 değerlerinin hassas popülasyonun LC50 değerine oranlanması ile belirlenmiştir. Çalışma sonucunda Tetranychus urticae’nin fasulye alanlarından toplanan B1, B2, B3, B4 ve B5 popülasyonlarında spiromesifen direnç oranları sırasıyla 98.1, 51.5, 177.1, 130.6 ve 53.6 kat; tebufenpyrad direnç oranları ise 2.3, 1.1, 1.8, 1.8 ve 1.0 kat olarak belirlenmiştir. Kırmızıörümcek popülasyonlarında spiromesifen ve tebufenpyrad arasında çapraz direnç belirlenmemiştir. Sonuçlar birlikte değerlendirildiğinde, bu alanlarda Tetranychus urticae mücadelesinde spiromesifen kullanılmaması gerektiği, tebufenpyrad kullanımının ise daha doğru olacağı düşünülmektedir.

Anahtar Kelimeler

• Çapraz direnç
• Tetranychus urticae
• Tebufenpyrad
• Spiromesifen

Kaynakça

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