Arpada Hücresel Apoptoz Hassasiyet (CAS) Geninin Susturularak Külleme Hastalığına Karşı Dirençte Etkisinin Belirlenmesi

Figen Ersoy

Arpada Hücresel Apoptoz Hassasiyet (CAS) Geninin Susturularak Külleme Hastalığına Karşı Dirençte Etkisinin Belirlenmesi

Öz

Ekonomik olarak önemli bir tahıl bitkisi olan arpa, dünyada ekim alanı açısından buğday, çeltik ve mısırdan sonra gelmektedir. Blumeria graminis f. sp. hordei (Bgh) tarafından oluşturulan külleme hastalığı arpanın en önemli hastalıklarından biridir. Hücresel Apoptoz Hassasiyet (Cellular apoptosis susceptiblity; CAS) geninin, arpada külleme hastalığı sırasında ekspresyonunun arttığı tespit edilmiştir. R geni tarafından yönetilen sinyal iletim mekanizmaları hakkında kısıtlı bilgi bulunduğu için, CAS proteininin külleme direnci veya hassasiyeti sırasındaki fonksiyonu anlaşılmaya çalışılmıştır. CAS proteininin patojene karşı oluşan tepki sırasında rolünü anlamak için Arpa Çizgili Mozaik Virüsü (BSMV) tarafından indüklenen gen susturma metodu uygulanmıştır. HvCAS geni susturulan bitki örneklerinde kontrol grubuna (BSMV:00) göre mRNA seviyesi % 54 azalmıştır. Fakat HvCAS geni susturulan bitki örneklerinde külleme hastalığına karşı direnç veya hassasiyet tepkisinde önemli bir değişim saptanmamıştır.

Anahtar Kelimeler

Arpa,bitki hastalık dirençi,Blumeria graminis f. sp. hordei,külleme,Hücresel Apoptoz Hassasiyeti (CAS),virüs ile uyarılan gen susturma (VIGS)

Kaynakça

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Ayrıntılar

Birincil Dil

Türkçe

Konular

Ziraat Mühendisliği

Bölüm

Araştırma Makalesi

Yazarlar

Figen Ersoy

Yayımlanma Tarihi

12 Ekim 2018

Gönderilme Tarihi

2 Şubat 2017

Kabul Tarihi

6 Haziran 2018

Yayımlandığı Sayı

Yıl 2018 Cilt: 13 Sayı: 1

IZ

https://izlik.org/JA84EK38DZ

Kaynak Göster

RIS / Bibtex

APA

Ersoy, F. (2018). Arpada Hücresel Apoptoz Hassasiyet (CAS) Geninin Susturularak Külleme Hastalığına Karşı Dirençte Etkisinin Belirlenmesi. Ziraat Fakültesi Dergisi, 13(1), 40-51. https://izlik.org/JA84EK38DZ