Determination of Relationship between Streptomycin Sülfate Susceptibility and Their Fatty Acid Methyl Esters Profiles of Pseudomonas syringae pv. syringae Strains Isolated from Pome Fruits in East Anatolian Region

Yıl 2008, Cilt: 3 Sayı: 2, 60 - 67, 01.12.2008

Recep Kotan Kenan Karagöz

Öz

In this study; a total of 20 Pseudomonas syringae pv. syringae strains, isolated from pome
fruits, were tested for their susceptibilities to streptomycin sulfate. In addition; the relationship
between streptomycin susceptibility of strains and their fatty acid methyl ester (FAME) profiles was
determined. According to these results, only one strain was determined to be resistant to streptomycin
(5.0%). Four different FAMEs groups of tested strains were determined by using MIS system
(Microbial Identification System). Difference between groups was changed from 16% to 60%. The
highest rate of resistance (5%) was in second group. Difference of this group from the others was that
12:0 2OH (% 6.60), 12:0 3OH (% 5.59) and 17:0 cyclo (% 11.43) fatty acids were higher than the
others. Consequently, we think that fatty acid methyl esters of bacterial strains can be used as an
indicator of antibiotic resistance. But, this relationship should be also tested for more Pseudomonas
syringae pv. syringae strains and different pathogen species.

Anahtar Kelimeler

Antibiotic, FAME, fatty acid, MIS, Pseudomonas syringae pv. syringae, streptomycin sulfate

Doğu Anadolu Bölgesinde Yumuşak Çekirdekli Meyvelerden İzole Edilen Pseudomonas syringae pv. syringae Strainlerinin Streptomisin Sülfata Karşı Duyarlılıkları ile Yağ Asidi Metil Esterleri Arasındaki İlişkinin Belirlenmesi

Öz

Bu çalışmada; yumuşak çekirdekli meyvelerden izole edilen toplam 20 Pseudomonas syringae
pv. syringae straininin streptomisin sülfata karşı duyarlılıkları test edilmiş, strainlerin yağ asidi metil
ester (FAME) grupları da belirlenerek patojenin yağ asitleri ve streptomisin sülfata duyarlılıkları
arasındaki ilişki araştırılmıştır. Elde edilen sonuçlara göre sadece 1 strainde (% 5.0) dayanıklılık
tespit edilmiştir. MIS (Mikrobiyal Tanı Sistemi) kullanılarak belirlenen yağ asidi metil esterleri
bakımından ise bu patojenin 4 farklı FAME grubunun olduğu belirlenmiştir. Gruplar arasındaki
farklılığın % 16 ile % 60 arasında değiştiği, dayanıklılık oranının ise 2. grupta en yüksek düzeyde
olduğu tespit edilmiştir. 12:0 2OH (% 6.60), 12:0 3OH (% 5.59) ve 17:0 cyclo (% 11.43) yağ asitleri
2. grupta diğer gruplara oranla daha yüksek oranda belirlenmiştir. Sonuç olarak; yağ asidi metil
esterlerinin bakterilerde antibiyotiklere duyarlılığın da bir göstergesi olarak kullanılabileceği
düşünülmektedir. Ancak, daha fazla Pseudomonas syringae pv. syringae strainlerinde ve farklı tür
patojenlerde de benzer çalışmaların yapılıp sonuçların değerlendirilmesi gerekmektedir.

Anahtar Kelimeler

Antibiyotik, FAME, yağ asidi, MIS, Pseudomonas syringae pv. syringae, streptomisin sülfat

Kaynakça

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