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Korkuteli Soğuk Hava Depolarında Armutta Penicillium expansum’a Karşı Bazı Alternatif Mücadele Yöntemlerinin Belirlenmesi

Yıl 2021, Cilt: 16 Sayı: 2, 226 - 233, 17.12.2021

Öz

Penicillium expansum'un neden olduğu mavi küf hastalığı, armutların en önemli hasat sonrası hastalıklarından biridir. Bazı mikroorganizmalar ve uçucu yağlar P. expansum'un büyümesini engelleme yeteneğine sahiptir. Bu çalışmada Bu çalışma, 'Karyağdı’ armut çeşidinde Bacillus subtilis, Trichoderma harzianum ve kekik yağının antagonistik kapasitesini karakterize etmeyi amaçlamıştır. In vitro antogonizm testlerinde T. harzianum, B. subtilis’den daha etkili ve % engelleme oranları sırasıyla %55.97, %49.37 olarak tespit edilmiştir. Kekik yağının kontakt uygulamasında P. expansum'un misel gelişimi %59.62-100, fumigant uygulamasında % 54.17-100 oranında engellenmiştir. Meyve biyo-tahlillerinde 25°C’de B. subtilis, T. harzianum ve kekik yağı meyve çürümesini %31.69-72.37, +0 °C’de soğuk hava deposunda %22.29-66.83 oranları arasında azaltmıştır. Çürük lezyonlarını en fazla kekik yağı kontak 500 µl/L uygulaması azaltırken, en düşük etkiyi kekik yağı fumigant %1 uygulaması göstermiştir. Çalışmalar sonucunda hem kekik yağının hemde antagonistik mikroorganizmaların (B. subtilis, T. harzianum) çevre dostu olmaları nedeniyle, armutlarda hasat sonrası P. expansum hastalığına karşı kullanılmasının yararlı olacağı kanısına varılmıştır.

Kaynakça

  • Amin S, Ismail A, Kasnazany S, Mahmood H (2017). Biological control of post-harvest disease of blue mould (Penicillium expansum) of pear fruit by using antagonist microorganisms under laboratory and cold storage conditions. International Journal of Current Research in Biosciences and Plant Biology, 4, 20-31.
  • Amiri A, Dugas R, Pichot A, Bompei G (2008). In vitro and in vivo activity of eugenol oil (Eugenia caryophylata) against four important postharvest apple pathogens. International Journal of Food microbiology, 126, 13-19.
  • Anonim (2019). Trt Haber https://www.trthaber.com/haber/yasam/karyagdi- armudunda-bu-yil-verim-yuksek-439426.html (Son erişim tarihi:11.12.2019)
  • Antunes MDC, Cavaco AM (2010). The use of essential oils for postharvest decay control. A review. Flavour and Fragrance Journal 25, 351-366. doi:10.1002/ffj.1986
  • Arroyave JJ, Mosquera S, Villegas-Escobar V (2017). Biocontrol activity of Bacillus subtilis EA-CB0015 cells and lipopeptides against postharvest fungal pathogens. Biological Control 114, 195–200. doi: 10.1016/j.biocontrol.2017.08.014
  • Barnett HL, Hunter BB (1972). Illustrated Genera of Imperfect Fungi, Burgess Publishing Company, USA, 240 p.
  • Batta Y (2015). Production and Testing of biopesticide for control of postharvest mold infections on fresh fruits of apple and pear. Advances in Microbiology, 5, 787-796. doi: 10.4236/aim.2015.512083.
  • Baydar H (2007). Tıbbi, Aromatik ve Keyf Bitkileri Bilimi ve Teknolojisi. Süleyman Demirel Üniversitesi Ziraat Fakültesi, S.D.Ü. Yayın No: 51, 216 s.
  • Bogiang L, Tongfei L, Guozheng Q, Shiping T (2009). Ambient pH ınhibits spore germination of Penicillium expansum by ımpairing protein synthesis and folding: A Proteomic-Based Study. Journal of Proteome Research, 9, 298-307.
  • Bosquez-Molina E, Ronquillo-de Jesus E, Bautista-Banos S, Verde-Calvo JR, Morales-Lopez J (2010). Inhibitory effect of essential oils against Colletotrichum gloeosporioides and Rhizopus stolonifer in stored papaya fruit and their possible application in coatings. Postharvest Biology and Technology, 57, 132-137.
  • Camele I, Altieri L, De Martino L, De Feo V, Mancini E, Rana GL (2012). In vitro control of post-harvest fruit rot fungi by some plant essential oil components. International Journal of Molecular Sciences. 13(2), 2290-2300.
  • Çaltıllı O, Arıcı ŞE (2017). The determination of the efficacy of some microbial preperations against apple scab disease (Venturia inaequais Cke Wint) in Isparta. 3rd ASM, International congress of agriculture and environment, 16-18.11.2017, Kasım 16-18.
  • Dehestani-Ardakani M, Mostofi Y (2019). Postharvest application of chitosan and Thymus essential oil increase quality of the table grape cv. ‘Shahroudi’. Journal of Horticulture and Postharvest Research, 2(1), 31-42. doi: 10.22077/jhpr.2018.1444.101
  • Domsch KH, Gams V, Anderson TH (1980). Compendium of Soil Fungi. Academic Press, London, pp:859.
  • Droby S, Wisniewski M, Macarisin D, Wilson C (2009). Twenty years of postharvest biocontrol research: Is it time for a new paradigm? Postharvest Biology and Technology, 52(2), 137-145. doi: 10.1016/ j.postharvbio.2008.11.009.
  • Erdogan O, Benlioglu K (2010). Biological control of Verticillium wilt on cotton by the use of fluorescent Pseudomonas spp. under field conditions. Biological Control, 53, 39–45.
  • Feng W, Chen J, Zheng X, Liu Q (2011). Tyme oil to control Alternaria alternata in vitro and in vivo as fumigant and contact treatments. Food Control, 22, 78-81.
  • Frisvad CJ, Samson AR (2004). Polyphrasic taxonomy of Penicillium subgenus Penicillium: A guide to identification of food and air borne terverticillate Penicillia and their mycotoxins. Studies in Mycology, 49, 1-174.
  • Isman MB (2000). Plant essential oils for pest and disease management. Crop Protection, 19(8-10), 603-608.
  • Kalın A (2011). Antalya İlinin Elmalı ve Korkuteli ilçelerinde soğuk hava depolarında sorun olan hasat sonrası hastalıkların belirlenmesi. (Yüksek Lisans Tezi, Ege Üniversitesi Fen Bilimleri Enstitüsü)
  • Liu J, Wisniewski M, Artlip T, Sui Y, Droby S, Norelli J (2013). The potential role of PR-8 gene of apple fruit in the mode of action of the yeast antagonist, Candida oleophila, in postharvest biocontrol of Botrytis cinerea. Postharvest Biology and Technology 85, 203-209. doi: 10.1016/j.postharvbio.06.007
  • Medina-Cordova N, Rosales Mendoza S, Hernández-Montiel L, Angulo C (2018). The potential use of Debaryomyces hansenii for the biological control of pathogenic fungi in food. Biological Control, 121, 216-222. doi: 0.1016/j.biocontrol.2018.03.002
  • Marandi RJ, Hassani A, Ghosta Y, Abdollahi A, Pirzad A, Sefidkon F (2011). Control of Penicillium expansum and Botrytis cinerea on pear with Thymus kotschyanus, Ocimum basilicum and Rosmarinus officinalis essential oils. Journal of Medicinal Plant Research, 5(4), 626-634.
  • Morales H, Sanchis V, Usall J, Ramos AJ, Marín S (2008). Effect of biocontrol agents Candida sake and Pantoea agglomerans on Penicillium expansum growth and patulin accumulation in apples. International Journal of Food Microbiology, 122(1-2), 61-67. doi: 10.1016/j.ijfoodmicro.2007.11.056.
  • Nunes C (2012). Biological control of postharvest diseases of fruit. European Journal of Plant Pathology, 133, 181-196. doi: 10.1007/ s10658-011-9919-7
  • Pitt JI, Hocking AD (2009). Fungi and food spoilage. New York, NY: Springer
  • Punja Z, Rodríguez G, Tirajoh A (2016). Effects of Bacillus subtilis strain QST 713 and storage temperatures on post-harvest disease development on greenhouse tomatoes. Crop Protection, 84, 98-104.
  • Rosenberger DA (1990). Blue mold. In A. L. Jones and H. S. Aldwinkle (Eds) Compendium of apple and pear diseases. St. Paul, MN: APS Press.
  • Sellamuthu PS, Sivakumar D, Soundy P (2013). Essential oils control Postharvest Diseases. Journal of Food Safety, 33, 86-93. doi:10.1111/jfs.12026
  • Sharma R, Singh D, Singh R (2009). Biological control of postharvest diseases of fruits and vegetables by microbial antagonists: A review. Biological Control, 50, 205-221. doi: 10.1016/j.biocontrol.2009.05.001
  • Sivakumar D, Bautista-Baños S (2014). A review on the use of essential oils for postharvest decay control and maintenance of fruit quality during storage. Crop Protection. 64, 27-37. doi: 0.1016/j.cropro.2014.05.012

Determination of Some Alternative Methods Against Pear Penicillium expansum in Korkuteli Cold Storages

Yıl 2021, Cilt: 16 Sayı: 2, 226 - 233, 17.12.2021

Öz

The blue mold disease caused by Penicillium expansum is one of the most important post-harvest diseases of pears. Some microorganisms and essential oils have the ability to inhibit P. expansum from growing. This study aimed to characterize the antagonistic capacity of Bacillus subtilis, Trichoderma harzianum and thyme oil in the pear variety “Karyağdı”. In in vitro antagonism tests, T. harzianum was found to be more effective than Bacillus subtilis and the% inhibition rates were 55.97% and 49.37%, respectively. The mycelial growth of P. expansum was prevented by 59.62-100% with the contact application of thyme oil and 54.17-100% with the use of fumigants. In fruit bioassays, B. subtilis, T. harzianum and thyme oil reduced fruit rot under controlled conditions by 31.69-72.37 % at 25 ° C and by 22.29 - 66.83 at + 4 ° C. While the application of thyme oil contact with 500 µl / L reduced the caries lesions the most, the 1% application of thyme oil fumigant showed the least effect. As a result of the studies, it was concluded that it would be beneficial to use thyme oil and antagonistic microorganisms (B. subtilis, T. harzianum) against P. expansum disease after harvest in pears due to their environmental friendliness.

Kaynakça

  • Amin S, Ismail A, Kasnazany S, Mahmood H (2017). Biological control of post-harvest disease of blue mould (Penicillium expansum) of pear fruit by using antagonist microorganisms under laboratory and cold storage conditions. International Journal of Current Research in Biosciences and Plant Biology, 4, 20-31.
  • Amiri A, Dugas R, Pichot A, Bompei G (2008). In vitro and in vivo activity of eugenol oil (Eugenia caryophylata) against four important postharvest apple pathogens. International Journal of Food microbiology, 126, 13-19.
  • Anonim (2019). Trt Haber https://www.trthaber.com/haber/yasam/karyagdi- armudunda-bu-yil-verim-yuksek-439426.html (Son erişim tarihi:11.12.2019)
  • Antunes MDC, Cavaco AM (2010). The use of essential oils for postharvest decay control. A review. Flavour and Fragrance Journal 25, 351-366. doi:10.1002/ffj.1986
  • Arroyave JJ, Mosquera S, Villegas-Escobar V (2017). Biocontrol activity of Bacillus subtilis EA-CB0015 cells and lipopeptides against postharvest fungal pathogens. Biological Control 114, 195–200. doi: 10.1016/j.biocontrol.2017.08.014
  • Barnett HL, Hunter BB (1972). Illustrated Genera of Imperfect Fungi, Burgess Publishing Company, USA, 240 p.
  • Batta Y (2015). Production and Testing of biopesticide for control of postharvest mold infections on fresh fruits of apple and pear. Advances in Microbiology, 5, 787-796. doi: 10.4236/aim.2015.512083.
  • Baydar H (2007). Tıbbi, Aromatik ve Keyf Bitkileri Bilimi ve Teknolojisi. Süleyman Demirel Üniversitesi Ziraat Fakültesi, S.D.Ü. Yayın No: 51, 216 s.
  • Bogiang L, Tongfei L, Guozheng Q, Shiping T (2009). Ambient pH ınhibits spore germination of Penicillium expansum by ımpairing protein synthesis and folding: A Proteomic-Based Study. Journal of Proteome Research, 9, 298-307.
  • Bosquez-Molina E, Ronquillo-de Jesus E, Bautista-Banos S, Verde-Calvo JR, Morales-Lopez J (2010). Inhibitory effect of essential oils against Colletotrichum gloeosporioides and Rhizopus stolonifer in stored papaya fruit and their possible application in coatings. Postharvest Biology and Technology, 57, 132-137.
  • Camele I, Altieri L, De Martino L, De Feo V, Mancini E, Rana GL (2012). In vitro control of post-harvest fruit rot fungi by some plant essential oil components. International Journal of Molecular Sciences. 13(2), 2290-2300.
  • Çaltıllı O, Arıcı ŞE (2017). The determination of the efficacy of some microbial preperations against apple scab disease (Venturia inaequais Cke Wint) in Isparta. 3rd ASM, International congress of agriculture and environment, 16-18.11.2017, Kasım 16-18.
  • Dehestani-Ardakani M, Mostofi Y (2019). Postharvest application of chitosan and Thymus essential oil increase quality of the table grape cv. ‘Shahroudi’. Journal of Horticulture and Postharvest Research, 2(1), 31-42. doi: 10.22077/jhpr.2018.1444.101
  • Domsch KH, Gams V, Anderson TH (1980). Compendium of Soil Fungi. Academic Press, London, pp:859.
  • Droby S, Wisniewski M, Macarisin D, Wilson C (2009). Twenty years of postharvest biocontrol research: Is it time for a new paradigm? Postharvest Biology and Technology, 52(2), 137-145. doi: 10.1016/ j.postharvbio.2008.11.009.
  • Erdogan O, Benlioglu K (2010). Biological control of Verticillium wilt on cotton by the use of fluorescent Pseudomonas spp. under field conditions. Biological Control, 53, 39–45.
  • Feng W, Chen J, Zheng X, Liu Q (2011). Tyme oil to control Alternaria alternata in vitro and in vivo as fumigant and contact treatments. Food Control, 22, 78-81.
  • Frisvad CJ, Samson AR (2004). Polyphrasic taxonomy of Penicillium subgenus Penicillium: A guide to identification of food and air borne terverticillate Penicillia and their mycotoxins. Studies in Mycology, 49, 1-174.
  • Isman MB (2000). Plant essential oils for pest and disease management. Crop Protection, 19(8-10), 603-608.
  • Kalın A (2011). Antalya İlinin Elmalı ve Korkuteli ilçelerinde soğuk hava depolarında sorun olan hasat sonrası hastalıkların belirlenmesi. (Yüksek Lisans Tezi, Ege Üniversitesi Fen Bilimleri Enstitüsü)
  • Liu J, Wisniewski M, Artlip T, Sui Y, Droby S, Norelli J (2013). The potential role of PR-8 gene of apple fruit in the mode of action of the yeast antagonist, Candida oleophila, in postharvest biocontrol of Botrytis cinerea. Postharvest Biology and Technology 85, 203-209. doi: 10.1016/j.postharvbio.06.007
  • Medina-Cordova N, Rosales Mendoza S, Hernández-Montiel L, Angulo C (2018). The potential use of Debaryomyces hansenii for the biological control of pathogenic fungi in food. Biological Control, 121, 216-222. doi: 0.1016/j.biocontrol.2018.03.002
  • Marandi RJ, Hassani A, Ghosta Y, Abdollahi A, Pirzad A, Sefidkon F (2011). Control of Penicillium expansum and Botrytis cinerea on pear with Thymus kotschyanus, Ocimum basilicum and Rosmarinus officinalis essential oils. Journal of Medicinal Plant Research, 5(4), 626-634.
  • Morales H, Sanchis V, Usall J, Ramos AJ, Marín S (2008). Effect of biocontrol agents Candida sake and Pantoea agglomerans on Penicillium expansum growth and patulin accumulation in apples. International Journal of Food Microbiology, 122(1-2), 61-67. doi: 10.1016/j.ijfoodmicro.2007.11.056.
  • Nunes C (2012). Biological control of postharvest diseases of fruit. European Journal of Plant Pathology, 133, 181-196. doi: 10.1007/ s10658-011-9919-7
  • Pitt JI, Hocking AD (2009). Fungi and food spoilage. New York, NY: Springer
  • Punja Z, Rodríguez G, Tirajoh A (2016). Effects of Bacillus subtilis strain QST 713 and storage temperatures on post-harvest disease development on greenhouse tomatoes. Crop Protection, 84, 98-104.
  • Rosenberger DA (1990). Blue mold. In A. L. Jones and H. S. Aldwinkle (Eds) Compendium of apple and pear diseases. St. Paul, MN: APS Press.
  • Sellamuthu PS, Sivakumar D, Soundy P (2013). Essential oils control Postharvest Diseases. Journal of Food Safety, 33, 86-93. doi:10.1111/jfs.12026
  • Sharma R, Singh D, Singh R (2009). Biological control of postharvest diseases of fruits and vegetables by microbial antagonists: A review. Biological Control, 50, 205-221. doi: 10.1016/j.biocontrol.2009.05.001
  • Sivakumar D, Bautista-Baños S (2014). A review on the use of essential oils for postharvest decay control and maintenance of fruit quality during storage. Crop Protection. 64, 27-37. doi: 0.1016/j.cropro.2014.05.012
Toplam 31 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Konular Ziraat Mühendisliği
Bölüm Araştıma
Yazarlar

Evrim Arıcı 0000-0001-5453-5869

Şahan Koç 0000-0002-7586-2428

Yayımlanma Tarihi 17 Aralık 2021
Gönderilme Tarihi 1 Kasım 2021
Kabul Tarihi 7 Aralık 2021
Yayımlandığı Sayı Yıl 2021 Cilt: 16 Sayı: 2

Kaynak Göster

APA Arıcı, E., & Koç, Ş. (2021). Korkuteli Soğuk Hava Depolarında Armutta Penicillium expansum’a Karşı Bazı Alternatif Mücadele Yöntemlerinin Belirlenmesi. Ziraat Fakültesi Dergisi, 16(2), 226-233.