Farklı CAD/CAM Blokların Sitotoksisite Değerlendirmesi

Abstract

Amaç: Bu çalışmanın amacı, farklı firmalar tarafından üretilen Computer Aided Design (CAD) ve Computer Aided Manufacturing (CAM) bloklarının insan periferik kan lenfositleri üzerindeki sitotoksik etkilerini MTT testi ile belirlemektir. Yöntem: Altı farklı CAD/CAM materyali araştırıldı: feldspat seramik VM (Vitablocks Mark II), rezin esaslı nano-seramik LU (Lava Ultimate), hibrit seramik C (Cerasmart), lösitle güçlendirilmiş cam seramik LRF (GC LRF), zirkonya ile güçlendirilmiş lityum silikat seramik VS (Vita Suprinity), polimer infiltrasyonu ile güçlendirilmiş cam seramik VE (Vita Enamic). CAD/CAM bloklardan toplam 36 disk şeklinde numune (Ø: 5 mm; h: 2 mm) hazırlandı. Hücre proliferasyonu ve sitotoksisite, MTT testi kullanılarak 24. ve 48. saatlerde değerlendirildi. Veriler İki Yönlü ANOVA testi ile istatistiksel olarak değerlendirildi (p < 0.05). Bulgular: 24 saatteki MTT canlılık verileri, grup VM ve LU bloklarının hafif sitotoksik olduğunu gösterdi, ancak diğer gruplarla karşılaştırıldığında istatistiksel olarak anlamlı bir fark yoktu (p>0.05). Tüm blokların 48 saat sonra hücre çoğalmasına neden olduğu belirlendi (p>0.05). Sitotoksik etkileri araştırılan tüm blokların farklı uygulama sürelerinde (24 saat ve 48 saat) herhangi bir toksik etkiye (24 saat VM ve LU hariç) neden olmadığı gösterildi. Sonuç: Elde edilen sonuçlara ve mevcut in vitro çalışmanın sınırlamalarına dayanarak, test edilen materyaller sitotoksik değildi. 24 saatlik sonuçlarda VM ve LU ihmal edilebilir sitotoksisiteye neden oldu.

Keywords

• insan periferik lenfositleri
• sitotoksisite
• CAD/CAM blokları

Cytotoxicity Evaluation of Different Types of CAD/CAM Blocks

Abstract

Aim: The aim of this study is to determine the cytotoxic effects of Computer Aided Design (CAD) and Computer Aided Manufacturing (CAM) blocks produced by different companies on human peripheral blood lymphocytes by MTT assay. Methods: Six different CAD/CAM materials were investigated: feldspar ceramic VM (Vitablocks Mark II ), resin nano-ceramic LU (Lava Ultimate), hybrid ceramic C (Cerasmart), leucite-reinforced ceramic LRF (GC LRF), zirconia-reinforced lithium silicate ceramic VS (Vita Suprinity), polymer-infiltrated ceramic-network VE (Vita Enamic). A total of 36 disc-shaped samples (Ø: 5 mm; h: 2 mm) were prepared from commercial blanks and blocks. Cell proliferation and cytotoxicity were assessed at 24h and 48h using MTT assay. The data were statistically evaluated with the Two-way ANOVA test (p < 0.05). Results: MTT viability data at 24 h showed that group VM and LU blocks were mildly cytotoxic, but there was no statistically significant difference when compared with other groups (p>0.05). It was determined that all blocks caused cell proliferation after 48 hours of exposure (p>0.05). It has been shown that all blocks whose cytotoxic effects were investigated did not cause any toxic effects (except VM and LU for 24 h) at different application times (24 h and 48 h). Conclusion: Based on the results obtained and the limitations of the current in vitro study, the tested materials were not cytotoxic. Only VM and LU caused negligible cytotoxicity at 24-hour exposure.

Keywords

• human peripheral lymphocytes
• cytotoxicity
• CAD/CAM blocks

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