Diyot lazer uygulamasının kök yüzeyi karbon, kalsiyum ve fosfor seviyelerine etkisi: Bir SEM-EDX çalışması

Abstract

Amaç: Periodontal tedavi sırasında kök yüzeylerindeki etkilenmemiş organik unsurların korunması gerekliliği literatürde vurgulanmaktadır. SEM-EDX analizi ile karbon, kalsiyum ve fosfor element seviyelerinin değerlendirilmesi, kök yüzeyindeki organik ve inorganik yapıların tayininde uygulanan bir yaklaşımdır. Bu çalışmanın amacı, periodontal cep dekontaminasyonu enerji düzeyinde gerçekleştirilen diyot lazer uygulamasının kök yüzeyindeki organik içeriğe etkisinin SEM-EDX yöntemi ile incelenmesidir.

Gereç ve Yöntemler:Ortodontik nedenlerden ötürü çekilmiş olan toplam 10 adet dişin her bir aproksimal yüzeyi test (n=20) ve kontrol (n=20) alanları olmak üzere iki bölgeye ayrıldı. Test bölgelerine diyot lazer (810 nm) uygulamaları gerçekleştirildi. Bütün örnekler karbon, kalsiyum ve fosfor element seviyeleri açısından SEM-EDX yöntemi ile analiz edildi.

Bulgular: Diyot lazer uygulanan kök yüzeylerinde, kontrol alanlarına göre anlamlı düzeyde düşük karbon seviyeleri gözlendi. Kalsiyum ve fosfor seviyeleri test bölgelerinde kontrol alanlarında göre daha yüksek seviyede gözlendi.

Sonuçlar: Bu çalışmanın sınırları kapsamında, diyot lazer uygulamalarının kök yüzeyindeki organik yapıya etki edebileceği ortaya konmuştur. Diyot lazerinin kök yüzeyindeki spesifik protein yapılarına olan etkilerini inceleyen sonraki çalışmalara ihtiyaç vardır.

Anahtar Kelimeler: Diyod lazer; kök yüzeyi; SEM-EDX; element analizi

Keywords

• diyot lazer,kök yüzeyi,SEM-EDX,element analizi

Effect of diode laser application on root surface carbon, calcium and phosphorus levels: A SEM-EDX study

Abstract

Background:Preservation of the organic ingredients on the root surfaces during the periodontal therapy has been emphasized in the literature. SEM-EDX analysis is an accepted approach to evaluate organic and inorganic components on investigated samples in terms of carbon, calcium and phosphor levels. The aim of this study was to evaluate the effects of diode laser application with periodontal pocket decontamination energy setting to the organic content of intact root surfaces via SEM-EDX analysis.

Methods: Each proximal root surfaces of the root surfaces of 10 teeth which were extracted due to orthodontic reasons were divided as test (n=20) and control (n=20) sites. Diode laser (810 nm) applications were done to the test sites of root surfaces. All specimens were analyzed using SEM-EDX with respect to carbon, calcium and phosphor levels.

Results:Diode laser applied root surfaces exhibit significantly lower carbon levels comparing with control sites. Additionally, increased calcium and phosphor levels were detected in the test sites.

Conclusions:Within the limitations of this study, it can be concluded that, diode laser applications may alter organic content of intact root surface. Further studies are required to elucidate potential effects of diode laser application to specific protein components on the root surfaces.

Keywords: Diode laser, root surface, SEM-EDX, element levels          

Keywords

• Diode laser,root surface,SEM-EDX,element analysis

References

1. Referans1. Rossmann JA, Cobb CM. Lasers in periodontal therapy. Periodontol 2000 1995;9:150-164.
2. Referans2. Parker S. Lasers and soft tissue: periodontal therapy. Br Dent J 2007;202:309-315.
3. Referans3. Passanezi E, Damante CA, de Rezende ML, Greghi SL. Lasers in periodontal therapy. Periodontol 2000 2015;67:268-291.
4. Referans4. Greenstein G. Periodontal response to mechanical non-surgical therapy: a review. J Periodontol 1992;63:118-130.
5. Referans5. Qadri T, Javed F, Johannsen G, Gustafsson A. Role of diode lasers (800-980 nm) as adjuncts to scaling and root planing in the treatment of chronic periodontitis: a systematic review. Photomed Laser Surg 2015;33:568-575.
6. Referans6. Gehrig JS, Sroda R, Saccuzzo D. Fundamentals of periodontal instrumentation & advanced root instrumentation: p.
7. Referans7. Grzesik WJ, Narayanan AS. Cementum and periodontal wound healing and regeneration. Crit Rev Oral Biol Med 2002;13:474-484.
8. Referans8. MacNeil RL, Somerman MJ. Molecular factors regulating development and regeneration of cementum. J Periodontal Res 1993;28:550-559.

9. Referans9. Maruyama H, Aoki A, Sasaki KM, et al. The effect of chemical and/or mechanical conditioning on the Er:YAG laser-treated root cementum: analysis of surface morphology and periodontal ligament fibroblast attachment. Lasers Surg Med 2008;40:211-222.
10. Referans10. Ayo-Yusuf OA, Driessen CH, Botha AJ. SEM-EDX study of prepared human dentine surfaces exposed to gingival retraction fluids. J Dent 2005;33:731-739.
11. Referans11. Euzebio Alves VT, de Andrade AK, Toaliar JM, et al. Clinical and microbiological evaluation of high intensity diode laser adjutant to non-surgical periodontal treatment: a 6-month clinical trial. Clin Oral Investig 2013;17:87-95.
12. Referans12. Brudevold F, Steadman LT, Smith FA. Inorganic and organic components of tooth structure. Ann N Y Acad Sci 1960;85:110-132.
13. Referans13. LeGeros RZ. Calcium phosphates in oral biology and medicine. Monogr Oral Sci 1991;15:1-201.
14. Referans14. Ho SP, Yu B, Yun W, Marshall GW, Ryder MI, Marshall SJ. Structure, chemical composition and mechanical properties of human and rat cementum and its interface with root dentin. Acta Biomater 2009;5:707-718.
15. Referans15. Macneil RL, Sheng N, Strayhorn C, Fisher LW, Somerman MJ. Bone Sialoprotein Is Localized to the Root Surface during Cementogenesis. Journal of Bone and Mineral Research 1994;9:1597-1606.
16. Referans16. Proye MP, Polson AM. Effect of root surface alterations on periodontal healing. I. Surgical denudation. J Clin Periodontol 1982;9:428-440.
17. Referans17. Blomlof JP, Blomlof LB, Lindskog SF. Smear removal and collagen exposure after non-surgical root planing followed by etching with an EDTA gel preparation. J Periodontol 1996;67:841-845.
18. Referans18. Polson AM, Caton J. Factors influencing periodontal repair and regeneration. J Periodontol 1982;53:617-625.
19. Referans19. Schwarz F, Sculean A, Georg T, Reich E. Periodontal treatment with an Er: YAG laser compared to scaling and root planing. A controlled clinical study. J Periodontol 2001;72:361-367.
20. Referans20. Morlock BJ, Pippin DJ, Cobb CM, Killoy WJ, Rapley JW. The effect of Nd:YAG laser exposure on root surfaces when used as an adjunct to root planing: an in vitro study. J Periodontol 1992;63:637-641.
21. Referans21. Kreisler M, Al Haj H, Daublander M, et al. Effect of diode laser irradiation on root surfaces in vitro. J Clin Laser Med Surg 2002;20:63-69.
22. Referans22. Theodoro LH, Haypek P, Bachmann L, et al. Effect of ER:YAG and diode laser irradiation on the root surface: morphological and thermal analysis. J Periodontol 2003;74:838-843.
23. Referans23. Henmi A, Okata H, Anada T, et al. Bone matrix calcification during embryonic and postembryonic rat calvarial development assessed by SEM-EDX spectroscopy, XRD, and FTIR spectroscopy. J Bone Miner Metab 2016;34:41-50.
24. Referans24. Arnold WH, Gaengler P. Quantitative analysis of the calcium and phosphorus content of developing and permanent human teeth. Ann Anat 2007;189:183-190.