‘Self-Cure’ Üniversal Adezivin Dentine Olan Mikrogerilim Bağlanma Dayanımı: İn Vitro çalışma

Abstract

Amaç: Farklı yüzey hazırlama prosedürleri ve yaşlandırma sonrası, kendi kendine polimerize olabilen (self-cure) bir adezivin, iki farklı üniversal adeziv ile dentine olan mikrogerilim bağlanma dayanımının (µGBD) karşılaştırmalı olarak değerlendirilmesidir. Gereç ve Yöntemler: Çalışmada, 18 adet çekilmiş insan üçüncü azı dişi kullanıldı. Dişlerin okluzal mine yüzeyleri uzaklaştırıldı ve orta-koronaldeki dentin yüzeyleri elde edildi. Örnekler kullanılan yüzey hazırlama prosedürlerine göre iki gruba (Etch&Rinse ve Self-Etch), ardından uygulanacak adeziv sisteme göre üç alt gruba ayrıldı: 1) Clearfil Universal Bond Quick, 2) G-Premio Bond, 3) Tokuyama Universal Bond. Adeziv uygulanan dentin yüzeyine 4 mm yükseklikte kompozit rezin uygulandı ve polimerize edildi. Örnekler 24 saat 37 °C nemli ortamda bekletildikten sonra düşük hızlı kesme cihazı (Micracut 125, Metkon, Bursa, Türkiye) kullanılarak yaklaşık 1 mm2’lik kesitler elde edildi (n=15). 24 saat veya 6 ay suda yaşlandırma sonrası örneklerin mikrogerilim bağlanma testi (Microtensile Tester, Bisco, IL, ABD) 0.5 mm/dk bir hızda gerçekleştirildi ve veriler MPa cinsinden kaydedildi. Veriler Kruskal-Wallis, Mann-Whitney U ve bağımsız örneklem t-test kullanılarak analiz edildi. Kopma tipleri stereomikroskop altında incelendi. Bulgular: Tüm adezivlerde ER prosedürü istatistiksel olarak anlamlı şekilde yüksek μGBD gösterdi (p<0.001). SE prosedürü uygulanan gruplarda yaşlandırma sonrası adezivlerin μGBD değerleri arasında anlamlı fark görülmedi. (p>0.05). ER prosedürü uygulanan gruplarda yaşlandırma sonrası, TUB hariç (p=0.282) diğer adezivlerde istatistiksel olarak anlamlı olarak düşüş görüldü (p<0.001). Sonuç: SE prosedürü ile kullanılan üniversal adezivlerin yaşlandırma sonrası µGBD değerleri anlamlı fark göstermedi. ‘Self-cure’ adeziv TUB diğer adezivlerden daha düşük µGBD değerleri gösterse de hem ER hem SE prosedüründe yaşlandırma sonrası µGBD değerleri anlamlı fark göstermeyen tek adezivdi.

Keywords

• dentin bağlanma dayanımı
• mikrogerilim bağlanma dayanımı
• üniversal adeziv
• self-cure adeziv

Microtensile Bond Strength of 'Self-Cure' Universal Adhesive to Dentin: in vitro study

Abstract

Aim: The purpose of this study is to compare the microtensile dentin bond strength (µTBS) of the 'self-cure' adhesive to dentin with two universal adhesives after different surface preparation procedures and aging. Methods: In the study, 18 extracted human third molars were used. The occlusal enamel surfaces of the teeth were removed and the mid-coronal dentin surfaces were obtained. The samples were divided into two groups (Etch&Rinse and Self-Etch) according to the surface preparation procedures used, and then into three sub-groups according to the adhesive system to be applied: 1) Clearfil Universal Bond Quick, 2) G-Premio Bond, 3) Tokuyama Universal Bond. 4 mm composite resin was applied to the adhesive applied dentin surface and polymerized. After the samples were kept at 37 ° C for 24 hours, sections of approximately 1 mm2 (n=15) were obtained by using a low speed cutting device (Micracut 125, Metkon, Bursa, Turkey). Microtensile bond strength were performed at a crosshead speed of 0.5 mm/min using a test device (Microtensile Tester, Bisco, IL, USA) after 24 hours or 6 months of water aging and data were recorded in MPa. Data were analyzed using the Kruskal–Wallis test, Mann–Whitney U and independent samples t-test. Failure modes were analyzed under a stereomicroscope. Results: The ER procedure showed a statistically significant difference in all adhesives (p<0.001). There was no significant difference in µTBS values of adhesives after aging in the SE procedure groups (p>0.05). After aging in the ER procedure groups, a statistically significant decrease was observed in adhesives (p<0.001) except TUB (p=0.282). Conclusions: After aging µTBS values of the universal adhesives used with the SE procedure did not show a significant difference. Although 'self-cure' adhesive TUB showed lower µTBS values than other adhesives, it was the only adhesive that did not show a significant difference in µTBS values after aging in both ER and SE procedures.

Keywords

• dentin bond strength
• microtensile bond strength
• self-cure adhesive
• universal adhesive

References

1. 1. Miyazaki M, Tsujimoto A, Tsubota K, Takamizawa T, Kurokawa H, Platt JA. Important compositional characteristics in the clinical use of adhesive systems. J Oral Sci. 2014;56:1-9.
2. 1. Miyazaki M, Tsujimoto A, Tsubota K, Takamizawa T, Kurokawa H, Platt JA. Important compositional characteristics in the clinical use of adhesive systems. J Oral Sci. 2014;56:1-9.
3. 2. Reis A, Carrilho M, Breschi L, Loguercio AD. Overview of clinical alternatives to minimize the degradation of the resin-dentin bonds. Oper Dent. 2013;38:1-25.
4. 2. Reis A, Carrilho M, Breschi L, Loguercio AD. Overview of clinical alternatives to minimize the degradation of the resin-dentin bonds. Oper Dent. 2013;38:1-25.
5. 3. Miyazaki M, Tsubota K, Takamizawa T, Kurokawa H, Rikuta A, Ando S. Factors affecting the in vitro performance of dentin-bonding systems. Jpn Dent Sci Rev. 2012;48:53-60.
6. 3. Miyazaki M, Tsubota K, Takamizawa T, Kurokawa H, Rikuta A, Ando S. Factors affecting the in vitro performance of dentin-bonding systems. Jpn Dent Sci Rev. 2012;48:53-60.
7. 4. Pashley DH, Tay FR, Breschi L, Tjäderhane L, Carvalho RM, Carrilho M, et al. State of the art etch-and-rinse adhesives. Dent Mater. 2011;27:1-16.
8. 4. Pashley DH, Tay FR, Breschi L, Tjäderhane L, Carvalho RM, Carrilho M, et al. State of the art etch-and-rinse adhesives. Dent Mater. 2011;27:1-16.

9. 5. Van Meerbeek B, Yoshihara K, Yoshida Y, Mine A, De Munck J, Van Landuyt KL. State of the art of self-etch adhesives. Dent Mater. 2011;27:17-28.
10. 5. Van Meerbeek B, Yoshihara K, Yoshida Y, Mine A, De Munck J, Van Landuyt KL. State of the art of self-etch adhesives. Dent Mater. 2011;27:17-28.
11. 6. Yoshihara K, Yoshida Y, Hayakawa S, Nagaoka N, Irie M, Ogawa T, et al. Nanolayering of phosphoric acid ester monomer on enamel and dentin. Acta Biomater. 2011;7:3187-95.
12. 6. Yoshihara K, Yoshida Y, Hayakawa S, Nagaoka N, Irie M, Ogawa T, et al. Nanolayering of phosphoric acid ester monomer on enamel and dentin. Acta Biomater. 2011;7:3187-95.
13. 7. Yaguchi T. Layering mechanism of MDP-Ca salt produced in demineralization of enamel and dentin apatite. Dent Mater. 2017;33:23-32.
14. 7. Yaguchi T. Layering mechanism of MDP-Ca salt produced in demineralization of enamel and dentin apatite. Dent Mater. 2017;33:23-32.
15. 8. Jacker-Guhr S, Sander J, Luehrs AK. How “Universal” is Adhesion? Shear bond strength of multi-mode adhesives to enamel and dentin. J Adhes Dent. 2019;21:87-95.
16. 8. Jacker-Guhr S, Sander J, Luehrs AK. How “Universal” is Adhesion? Shear bond strength of multi-mode adhesives to enamel and dentin. J Adhes Dent. 2019;21:87-95.
17. 9. Chen C, Niu LN, Xie H, Zhang ZY, Zhou LQ, Jiao K. et al. Bonding of universal adhesives to dentine-Old wine in new bottles? J Dent. 2015;43:525-36.
18. 9. Chen C, Niu LN, Xie H, Zhang ZY, Zhou LQ, Jiao K. et al. Bonding of universal adhesives to dentine-Old wine in new bottles? J Dent. 2015;43:525-36.
19. 10. Saikaew P, Chowdhury AFMA, Fukuyama M, Kakuda S, Carvalho RM, Sano H. The effect of dentine surface preparation and reduced application time of adhesive on bonding strength. J Dent. 2016;47:63-70.
20. 10. Saikaew P, Chowdhury AFMA, Fukuyama M, Kakuda S, Carvalho RM, Sano H. The effect of dentine surface preparation and reduced application time of adhesive on bonding strength. J Dent. 2016;47:63-70.
21. 11. Sai K, Takamizawa T, Imai A, Tsujimoto A, Ishii R, Barkmeier WW, et al. Influence of application time and etching mode of universal adhesives on enamel adhesion. J Adhes Dent. 2018;20:65-77.
22. 11. Sai K, Takamizawa T, Imai A, Tsujimoto A, Ishii R, Barkmeier WW, et al. Influence of application time and etching mode of universal adhesives on enamel adhesion. J Adhes Dent. 2018;20:65-77.
23. 12. Hanabusa M, Mine A, Kuboki T, Momoi Y, Van Ende A, Van Meerbeek B, et al. Bonding effectiveness of a new “multi-mode” adhesive to enamel and dentine. J Dent. 2012;40:475-84.
24. 12. Hanabusa M, Mine A, Kuboki T, Momoi Y, Van Ende A, Van Meerbeek B, et al. Bonding effectiveness of a new “multi-mode” adhesive to enamel and dentine. J Dent. 2012;40:475-84.
25. 13. Muñoz MA, Sezinando A, Luque-Martinez I, Szesz AL, Reis A, Loguercio AD, et al. Influence of a hydrophobic resin coating on the bonding efficacy of three universal adhesives. J Dent. 2014;42:595-602.
26. 13. Muñoz MA, Sezinando A, Luque-Martinez I, Szesz AL, Reis A, Loguercio AD, et al. Influence of a hydrophobic resin coating on the bonding efficacy of three universal adhesives. J Dent. 2014;42:595-602.
27. 14. Yoshida Y, Yoshihara K, Nagaoka N, Hanabusa M, Matsumoto T, Momoi Y. X-ray diffraction analysis of three-dimensional self-reinforcing monomer and its chemical interaction with tooth and hydroxyapatite. Dent Mater J. 2012;31:697-702.
28. 14. Yoshida Y, Yoshihara K, Nagaoka N, Hanabusa M, Matsumoto T, Momoi Y. X-ray diffraction analysis of three-dimensional self-reinforcing monomer and its chemical interaction with tooth and hydroxyapatite. Dent Mater J. 2012;31:697-702.
29. 15. Tunaç AT, Can E. Universal Adhesive Systems. Turkiye Klin J Dent Sci. 2020;26:496-503.
30. 15. Tunaç AT, Can E. Universal Adhesive Systems. Turkiye Klin J Dent Sci. 2020;26:496-503.
31. 16. Yoshida Y, Nagakane K, Fukuda R, Nakayama Y, Okazaki M, Shintani H, et al. Comparative Study on Adhesive Performance of Functional Monomers. J Dent Res. 2004;83:454-8.
32. 16. Yoshida Y, Nagakane K, Fukuda R, Nakayama Y, Okazaki M, Shintani H, et al. Comparative Study on Adhesive Performance of Functional Monomers. J Dent Res. 2004;83:454-8.
33. 17. Van Landuyt KL, Snauwaert J, De Munck J, Peumans M, Yoshida Y, Poitevin A, et al. Systematic review of the chemical composition of contemporary dental adhesives. Biomaterials. 2007;28:3757-85.
34. 17. Van Landuyt KL, Snauwaert J, De Munck J, Peumans M, Yoshida Y, Poitevin A, et al. Systematic review of the chemical composition of contemporary dental adhesives. Biomaterials. 2007;28:3757-85.
35. 18. Nikaido T, Ichikawa C, Li N, Takagaki T, Sadr A, Yoshida Y, et al. Effect of functional monomers in all-in-one adhesive systems on formation of enamel/dentin acid-base resistant zone. Dent Mater J. 2011;30:576-82.
36. 18. Nikaido T, Ichikawa C, Li N, Takagaki T, Sadr A, Yoshida Y, et al. Effect of functional monomers in all-in-one adhesive systems on formation of enamel/dentin acid-base resistant zone. Dent Mater J. 2011;30:576-82.
37. 19. Tokuyama Dental Corporation Technical Report Ver. 1.2.; 2017.
38. 19. Tokuyama Dental Corporation Technical Report Ver. 1.2.; 2017.
39. 20. Armstrong S, Breschi L, Özcan M, Pfefferkorn F, Ferrari M, Van Meerbeek B. Academy of Dental Materials guidance on in vitro testing of dental composite bonding effectiveness to dentin/enamel using micro-tensile bond strength (μTBS) approach. Dent Mater. 2017;33:133-43.
40. 20. Armstrong S, Breschi L, Özcan M, Pfefferkorn F, Ferrari M, Van Meerbeek B. Academy of Dental Materials guidance on in vitro testing of dental composite bonding effectiveness to dentin/enamel using micro-tensile bond strength (μTBS) approach. Dent Mater. 2017;33:133-43.
41. 21. Van Meerbeek B, Peumans M, Poitevin A, Mine A, Van Ende A, Neves A, et al. Relationship between bond-strength tests and clinical outcomes. Dent Mater. 2010;26:100-21.
42. 21. Van Meerbeek B, Peumans M, Poitevin A, Mine A, Van Ende A, Neves A, et al. Relationship between bond-strength tests and clinical outcomes. Dent Mater. 2010;26:100-21.
43. 22. Donmez N, Siso SH, Usumez A, Bayrak I. Effect of thermal cycling on micro-tensile bond strength of composite restorations bonded with multimode adhesive. J Adhes Sci Technol. 2015;29:731-9.
44. 22. Donmez N, Siso SH, Usumez A, Bayrak I. Effect of thermal cycling on micro-tensile bond strength of composite restorations bonded with multimode adhesive. J Adhes Sci Technol. 2015;29:731-9.
45. 23. Leite ML de A e. S, Costa CA de S, Duarte RM, de Andrade AKM, Soares DG. Bond strength and cytotoxicity of a universal adhesive according to the hybridization strategies to dentin. Braz Dent J. 2018;29:68-75.
46. 23. Leite ML de A e. S, Costa CA de S, Duarte RM, de Andrade AKM, Soares DG. Bond strength and cytotoxicity of a universal adhesive according to the hybridization strategies to dentin. Braz Dent J. 2018;29:68-75.
47. 24. Wagner A, Wendler M, Petschelt A, Belli R, Lohbauer U. Bonding performance of universal adhesives in different etching modes. J Dent. 2014;42:800-7.
48. 24. Wagner A, Wendler M, Petschelt A, Belli R, Lohbauer U. Bonding performance of universal adhesives in different etching modes. J Dent. 2014;42:800-7.
49. 25. Saito T, Takamizawa T, Ishii R, Tsujimoto A, Hirokane E, Barkmeier WW, et al. Influence of Application Time on Dentin Bond Performance in Different Etching Modes of Universal Adhesives. Oper Dent. 2020;45:183-95.
50. 25. Saito T, Takamizawa T, Ishii R, Tsujimoto A, Hirokane E, Barkmeier WW, et al. Influence of Application Time on Dentin Bond Performance in Different Etching Modes of Universal Adhesives. Oper Dent. 2020;45:183-95.
51. 26. Da Rosa WLDO, Piva E, Da Silva AF. Bond strength of universal adhesives: A systematic review and meta-analysis. J Dent. 2015;43:765-76.
52. 26. Da Rosa WLDO, Piva E, Da Silva AF. Bond strength of universal adhesives: A systematic review and meta-analysis. J Dent. 2015;43:765-76.
53. 27. Saikaew P, Matsumoto M, Chowdhury AFMA, Carvalho RM, Sano H. Does shortened application time affect long-term bond strength of universal adhesives to dentin? Oper Dent. 2018;43:549-58.
54. 27. Saikaew P, Matsumoto M, Chowdhury AFMA, Carvalho RM, Sano H. Does shortened application time affect long-term bond strength of universal adhesives to dentin? Oper Dent. 2018;43:549-58.
55. 28. Muñoz MA, Luque-Martinez I, Malaquias P, Hass V, Reis A, Campanha NH, et al. In vitro longevity of bonding properties of universal adhesives to dentin. Oper Dent. 2015;40:282-92.
56. 28. Muñoz MA, Luque-Martinez I, Malaquias P, Hass V, Reis A, Campanha NH, et al. In vitro longevity of bonding properties of universal adhesives to dentin. Oper Dent. 2015;40:282-92.
57. 29. Armstrong SR, Vargas MA, Chung I, Pashley DH, Campbell JA, Laffoon JE, et al. Resin-dentin interfacial ultrastructure and microtensile dentin bond strength after five-year water storage. Oper Dent. 2004;29:705-712.
58. 29. Armstrong SR, Vargas MA, Chung I, Pashley DH, Campbell JA, Laffoon JE, et al. Resin-dentin interfacial ultrastructure and microtensile dentin bond strength after five-year water storage. Oper Dent. 2004;29:705-712.
59. 30. Hannas AR, Pereira JC, Granjeiro JM, Tjäderhane L. The role of matrix metalloproteinases in the oral environment. Acta Odontol Scand. 2007;65:1-13.
60. 30. Hannas AR, Pereira JC, Granjeiro JM, Tjäderhane L. The role of matrix metalloproteinases in the oral environment. Acta Odontol Scand. 2007;65:1-13.
61. 31. Zhang S chuan, Kern M. The role of host-derived dentinal matrix metalloproteinases in reducing dentin bonding of resin adhesives. Int J Oral Sci. 2009;1:163-76. 32. Imai A, Takamizawa T, Sai K, Tsujimoto A, Nojiri K, Endo H, et al. Influence of application method on surface free-energy and bond strength of universal adhesive systems to enamel. Eur J Oral Sci. 2017;125:385-95.
62. 31. Zhang S chuan, Kern M. The role of host-derived dentinal matrix metalloproteinases in reducing dentin bonding of resin adhesives. Int J Oral Sci. 2009;1:163-76. 32. Imai A, Takamizawa T, Sai K, Tsujimoto A, Nojiri K, Endo H, et al. Influence of application method on surface free-energy and bond strength of universal adhesive systems to enamel. Eur J Oral Sci. 2017;125:385-95.
63. 33. Takahashi M, Nakajima M, Hosaka K, Ikeda M, Foxton RM, Tagami J. Long-term evaluation of water sorption and ultimate tensile strength of HEMA-containing/-free one-step self-etch adhesives. J Dent. 2011;39:506-12.
64. 33. Takahashi M, Nakajima M, Hosaka K, Ikeda M, Foxton RM, Tagami J. Long-term evaluation of water sorption and ultimate tensile strength of HEMA-containing/-free one-step self-etch adhesives. J Dent. 2011;39:506-12.
65. 34. Kuno Y, Hosaka K, Nakajima M, Ikeda M, Klein CA, Foxton RM, et al. Incorporation of a hydrophilic amide monomer into a one-step self-etch adhesive to increase dentin bond strength: Effect of application time. Dent Mater J. 2019;38:892-9.
66. 34. Kuno Y, Hosaka K, Nakajima M, Ikeda M, Klein CA, Foxton RM, et al. Incorporation of a hydrophilic amide monomer into a one-step self-etch adhesive to increase dentin bond strength: Effect of application time. Dent Mater J. 2019;38:892-9.
67. 35. Hiraishi N, Tochio N, Kigawa T, Otsuki M, Tagami J. Monomer-collagen interactions studied by saturation transfer difference NMR. J Dent Res. 2013;92:284-8. 36. Yoshida Y, Yoshihara K, Nagaoka N, Hayakawa S, Torii Y, Ogawa T, et al. Self-assembled nano-layering at the adhesive interface. J Dent Res. 2012;91:376-81.
68. 35. Hiraishi N, Tochio N, Kigawa T, Otsuki M, Tagami J. Monomer-collagen interactions studied by saturation transfer difference NMR. J Dent Res. 2013;92:284-8. 36. Yoshida Y, Yoshihara K, Nagaoka N, Hayakawa S, Torii Y, Ogawa T, et al. Self-assembled nano-layering at the adhesive interface. J Dent Res. 2012;91:376-81.
69. 37. Breschi L, Mazzoni A, Ruggeri A, Cadenaro M, Di Lenarda R, De Stefano Dorigo E. Dental adhesion review: Aging and stability of the bonded interface. Dent Mater. 2008;24:90-101. 38. Van Landuyt KL, De Munck J, Snauwaert J, Coutinho E, Poitevin A, Yoshida, Y, et al. Monomer-Solvent Phase Separation in One-step Self-etch Adhesives. J Dent Res. 2005;84:183-8.
70. 37. Breschi L, Mazzoni A, Ruggeri A, Cadenaro M, Di Lenarda R, De Stefano Dorigo E. Dental adhesion review: Aging and stability of the bonded interface. Dent Mater. 2008;24:90-101. 38. Van Landuyt KL, De Munck J, Snauwaert J, Coutinho E, Poitevin A, Yoshida, Y, et al. Monomer-Solvent Phase Separation in One-step Self-etch Adhesives. J Dent Res. 2005;84:183-8.
71. 39. Itoh S, Nakajima M, Hosaka K, Okuma M, Takahashi M, Shinoda Y, et al. Dentin bond durability and water sorption/solubility of one-step self-etch adhesives. Dent Mater J. 2010;29:623-30.
72. 39. Itoh S, Nakajima M, Hosaka K, Okuma M, Takahashi M, Shinoda Y, et al. Dentin bond durability and water sorption/solubility of one-step self-etch adhesives. Dent Mater J. 2010;29:623-30.
73. 40. Daneshmehr L, Ozer F, Mante FK, Blatz MB. Relationship between air-blowing duration and bond strengths of three adhesive systems to dentin after thermal aging. Dent Mater J. 2013;32:767-774.
74. 40. Daneshmehr L, Ozer F, Mante FK, Blatz MB. Relationship between air-blowing duration and bond strengths of three adhesive systems to dentin after thermal aging. Dent Mater J. 2013;32:767-774.
75. 41. Oguri M, Yoshida Y, Yoshihara K, Miyauchi T, Nakamura Y, Shimoda S, et al. Effects of functional monomers and photo-initiators on the degree of conversion of a dental adhesive. Acta Biomater. 2012;8:1928-34.
76. 41. Oguri M, Yoshida Y, Yoshihara K, Miyauchi T, Nakamura Y, Shimoda S, et al. Effects of functional monomers and photo-initiators on the degree of conversion of a dental adhesive. Acta Biomater. 2012;8:1928-34.