Evaluation of Total Oxidant and Antioxidant Status of Various Pulp Capping Materials on Human Dental Pulp Stem Cells

Yıl 2020, Cilt: 7 Sayı: 2, 192 - 199, 01.08.2020

Seçkin Aksu , Taşkın Gürbüz

https://doi.org/10.15311/selcukdentj.498311

Cited By: 1

Öz

Background: The
aim of this study was to evaluate the total oxidant and antioxidant status of five
pulp capping materials (Mineral Trioxide Aggregate, Biodentine, Pulpine
Mineral, Endo Repair and Zinc Oxide Eugenol) on human dental pulp stem cells.

Methods: In our study, American Type Culture
Collection human dental pulp stem cells were used. The filter diffusion test
was applied according to ISO standards. At the end of 24^th and 72^nd
hours, biochemical analysis was conducted (n=3). Kruskal Wallis Variance
Analysis and Mann-Whitney U test were used for independent group comparisons
between measurements.

Results: As a result of 24 hours evaluation, only Mineral Trioxide Aggregate
exhibits antioxidative protection similar to control group (p> 0.05), while
other groups remain below this level. It was determined that the total
antioxidant status of all groups was increased between 24 and 72 hours
(p<0.05).  At the end of 72nd hours,
the best preservative groups were Biodentine and Pulpine Mineral, additionally
the weakest protective group was Zinc Oxide Eugenol (p<0.05).            

Conclusions: According to the analysis, it was
determined that all pulp capping materials have an oxidative effect on human
dental pulp stem cells and also have an antioxidant defense mechanism. The highest protection in the acute exposure was found
in the Mineral Trioxide Aggregate group and additionally, Biodentine and
Pulpine Mineral (includig propolis) groups were found to be the most protective
for a long time. The antioxidative effect of ZnOEu group was found to be
insufficient. The propolis containing pulp capping material has been shown to
be a good alternative to calcium phosphate products due to its positive effect
on stem cells.

Keywords: Dental pulp capping, calcium phosphate,
free radicals, propolis.

Anahtar Kelimeler

Dental pulp capping, calcium phosphate, free radicals, propolis

Farklı Pulpa Kaplama Materyallerinin Toplam Oksidan ve Antioksidan Kapasitelerinin İnsan Dental Pulpa Kök Hücreleri Üzerinde Değerlendirilmesi

Öz

Amaç: Bu çalışmanın amacı, beş farklı pulpa kaplama materyalinin (Mineral
Trioksit Agregat, Biodentin, Pulpine Mineral, Endo Repair
ve Çinko Oksit Öjenol) toplam oksidan ve
antioksidan seviyelerinin insan dental pulpa kök hücreleri üzerinde
değerlendirilmesidir.

Gereç ve Yöntemler:Çalışmamızda Amerikan Tipi
Kültür Koleksiyonu insan dental pulpa kök hücreleri kullanılmıştır. Filtre
difüzyon testi, ISO standartlarına göre uygulanmıştır. 24. ve 72. saatlerin
sonunda biyokimyasal analiz yapılmıştır(n = 3). Ölçümler arasında bağımsız grup
karşılaştırmaları için Kruskal Wallis Varyans Analizi ve Mann-Whitney U testi
kullanılmıştır.

Bulgular: 24 saatlik değerlendirme sonucunda, sadece Mineral Trioksit Agregat,
kontrol grubuna benzer antioksidatif koruma gösterirken (p> 0.05), diğer
gruplar bu seviyenin altında kalmıştır. Tüm grupların toplam antioksidan
durumunun 24 ve 72 saat arasında arttığı belirlenmiştir (p <0.05). 72 saatin
sonunda, en iyi koruma Biodentin ve Pulpine Mineral gruplarındai en zayıf
koruma ise Çinko Oksit Eugenol grubunda bulunmuştur (p <0.05).

Sonuçlar: Yapılan
analize göre, tüm pulpa kaplama materyallerinin insan dental pulpa kök
hücreleri üzerinde hem oksidatif etki hem de antioksidan savunma mekanizmasını
uyarıcı etkiye sahip olduğu belirlenmiştir. İlk 24 saatte en yüksek korunma Mineral Trioxide Aggregate grubunda, 72
saatte ise; Biodentin ve propolis içeren Pulpine Mineral gruplarında
bulunmuştur. ZnOEu grubunun antioksidatif etkisinin yetersiz olduğu tespit
edilmiştir. Propolis içerikli pulpa kaplama materyalinin kök hücreler
üzerindeki olumlu etkisi nedeniyle kalsiyum fosfat içerikli ürünlere iyi bir
alternatif olabileceği ortaya konulmuştur.

Anahtar Kelimeler: Diş pulpa kaplaması, kalsiyum fosfat, serbest radikaller, propolis.

Anahtar Kelimeler

Diş pulpa kaplaması, kalsiyum fosfat, serbest radikaller, propolis

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