Investigation for Mutation in BMPR-1B (FecB) Fecundity Gene in Awassi Sheep

Öz

Reproduction related traits in sheep such as ovulation rate and litter size are traits of high economic importance but as these traits are only expressed in one sex and in mature animals, thus inclusion of them in selection strategies is limited. Therefore, studying genes associated with reproductive events provides genotypic data, which is more useful in genetic improvement of sheep in a short period of time. Such reproductive traits in sheep are genetically controlled by genes having both additive and major effects. Booroola is the first gene identified in Booroola Merino sheep in Australia has major effects on ewe’s reproduction and has gained much popularity in sheep breeding for its immense economic value. Booroola or Bone Morphogenetic Protein Receptor -1B (BMPR-1B) gene is in chromosome 6 and FecB allele is the result of a single mutation in this gene where one copy of this allele results in a significant increase in ovulation rate thus outcome as increased lambing per parturition. The Awassi sheep is an indigenous breed of Turkey and identifying major genes for fecundity could greatly improve their breeding program. To identify the FecB gene in Awassi sheep, 88 blood samples were taken, and DNA was extracted by salting out method. After PCR amplification and AvaΠ digestion, the samples were analyzed for FecB mutation and all of 88 samples were found not to carry the mutant allele (FecB).

Anahtar Kelimeler

• Awassi
• BMPR-1B gene
• Booroola
• Litter size
• PCR-RFLP

Kaynakça

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