Salt Stress Tolerance of 'Hatay' Clonal Apple Rootstock Candidate (Malus sylvestris L. Mill. Subsp. orientalis) Under in vitro Conditions

Öz

Salt stress is an important environmental factor that negatively affects the growth and development of plants. The apple is one of the most sensitive plants to salt stress among fruit species. In this study, the tolerance of 'Hatay' clonal apple rootstock candidate to salt stress under in vitro conditions was investigated. Different salt concentrations (1/4 dilute MS, 1/2 dilute MS, MS (control), MS+1gL-1 NaCl, MS+2gL-1 NaCl, MS+4gL-1 NaCl, MS+6gL-1 NaCl, MS+8gL-1 NaCl, MS+10gL-1 NaCl) were used. Plant height, leaf area, stomatal conductance, fresh and dry weight, rooting rate, root number and root length were measured after salt stress. According to the study results, the highest plant height was determined in MS (control) medium and the lowest in 1/4 MS medium. The highest plant fresh weight was determined in MS (control) and MS+1gL-1 NaCl medium and lowest in 1/4 MS medium. The lowest plant fresh weight was determined in 1/4 MS medium. Leaf area was highest in MS medium and lowest in MS+1gL-1 NaCl medium. Stomatal conductance was highest in 1/2 MS medium and lowest in MS+1gL-1 NaCl medium. The rooting rate was highest in MS+1gL-1 NaCl medium and lowest in 1/4 MS medium. In terms of root number, root length, root fresh and dry weight, the best results were generally obtained in 1/2 MS medium, while the lowest results were obtained in 1/4 MS medium. The plants died in MS+2gL-1 NaCl, MS+4gL-1 NaCl, MS+6gL-1 NaCl, MS+8gL-1 NaCl, MS+10gL-1 NaCl environments. As a result, 'Hatay' clonal apple rootstock candidate was found to be sensitive to salt stress.

Anahtar Kelimeler

• apple
• rootstock candidate
• salt stress
• in vitro
• morphological characteristics

Destekleyen Kurum

Selçuk University Scientific Research Project Institute

Proje Numarası

24201037

Etik Beyan

No ethical declaration required.

'Hatay' Klonal Elma Anaç Adayının (Malus sylvestris L. Mill. Subsp. orientalis) in vitro Şartlarda Tuza Toleransı

Öz

Tuz stresi, bitkilerin büyüme ve gelişmesini olumsuz etkileyen önemli bir çevresel faktördür. Elma, meyve türleri arasında tuz stresine en duyarlı bitkilerden biridir. Bu çalışmada, 'Hatay' klonal elma anaç adayının in vitro koşullarda tuz stresine toleransı araştırılmıştır. Farklı tuz konsantrasyonları (1/4 seyreltik MS, 1/2 seyreltik MS, MS (kontrol), MS+1gL-1 NaCl, MS+2gL-1 NaCl, MS+4gL-1 NaCl, MS+6gL-1 NaCl, MS+8gL-1 NaCl, MS+10gL-1 NaCl) kullanılmıştır. Tuz stresi sonrasında bitki boyu, yaprak alanı, stoma iletkenliği, yaş ve kuru ağırlık, köklenme oranı, kök sayısı ve kök uzunluğu ölçülmüştür. Çalışma sonuçlarına göre en yüksek bitki boyu MS (kontrol) ortamında, en düşük ise 1/4 MS ortamında belirlenmiştir. En yüksek bitki yaş ve kuru ağırlığı MS+1gL-1 NaCl ortamında, en düşük ise 1/4 MS ortamında belirlenmiştir. Yaprak alanı MS ortamında en yüksek, MS+1gL-1 NaCl ortamında en düşük olmuştur. Stoma iletkenliği 1/2 MS ortamında en yüksek, MS+1gL-1 NaCl ortamında en düşük olmuştur. Köklenme oranı MS+1gL-1 NaCl ortamında en yüksek, 1/4 MS ortamında ise en düşük olmuştur. Kök sayısı, kök uzunluğu, kök yaş ve kuru ağırlığı bakımından en iyi sonuçlar genellikle 1/2 MS ortamında elde edilirken, en düşük sonuçlar 1/4 MS ortamında elde edilmiştir. Bitkiler MS+2gL-1 NaCl, MS+4gL-1 NaCl, MS+6gL-1 NaCl, MS+8gL-1 NaCl, MS+10gL-1 NaCl ortamlarında ölmüştür. Sonuç olarak 'Hatay' klon elma anaç adayının tuz stresine duyarlı olduğu bulunmuştur.

Anahtar Kelimeler

• elma
• anaç adayı
• tuz stresi
• in vitro
• morfolojik özellikler

Destekleyen Kurum

Selçuk Üniversitesi Bilimsel Araştırma Projeleri Koordinatörlüğü

Proje Numarası

24201037

Etik Beyan

Etik beyana gerek yoktur.

Kaynakça

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