Araştırma Makalesi
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Lityumun $Achroia$ $grisella$ Fabr. (Lepidoptera: Pyralidae) Larvalarının Protein, Lipit ve Karbohı̇drat Miktarına Etkı̇sı̇

Yıl 2025, Cilt: 10 Sayı: 2, 401 - 409, 24.12.2025
https://doi.org/10.33484/sinopfbd.1651126

Öz

Bu çalışmada, lityumun Küçük Balmumu Güvesi larvalarının protein, lipit ve karbohidrat miktarlarına etkisi incelenmiştir. Larvalar farklı konsantrasyonlarda lityum (125, 250 ve 500 mg/kg) ile kontamine edilen yapay bir besin ile beslenmiş ve biyokimyasal analizlerde son evre larvalar kullanılmıştır. Tüm böcekler 25 ± 2 °C sıcaklık, %60 ± 5 bağıl nem ve 16A:8K fotoperiyot koşullarında yetiştirilmiştir. Lityum ile muamele 125 ve 500 mg/kg konsantrasyonlarda larvaların protein miktarlarını düşürdüğü halde, larvaların lipit düzeyleri aynı lityum konsantrasyonlarında önemli ölçüde artmıştır. Buna karşın lityum larvaların karbonhidrat seviyesinde önemli bir farklılığa neden olmamıştır.

Etik Beyan

Çalışma, etik kurul izni veya herhangi bir özel izin gerektirmemektedir.

Destekleyen Kurum

Çalışma Ondokuz Mayıs Üniversitesi tarafından BAP04-A-2023-4752 proje numarası ile yüksek lisans tez projesi olarak desteklenmiştir

Proje Numarası

BAP04-A-2023-4752

Teşekkür

Bu çalışmayı BAP04-A-2023-4752 proje numarası ile Yüksek Lisans Tez Projesi olarak destekleyen Ondokuz Mayıs Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi’ne teşekkür ederiz.

Kaynakça

  • Tanveer, M., Hasanuzzaman, M., & Wang, L. (2019). Lithium in environment and potential targets to reduce lithium toxicity in plants. Journal of Plant Growth Regulation, 38(4), 1574-1586. https://doi.org/10.1007/s00344-019-09957-2
  • Aral, H., & Vecchio-Sadus, A. (2008). Toxicity of lithium to humans and the environment—a literature review. Ecotoxicology and Environmental Safety, 70(3), 349-356. https://doi.org/10.1016/j.ecoenv.2008.02.026
  • US Geological Survey. (2020). Mineral commodity summaries 2020. US Geological Survey, 200p. https://doi.org/10.3133/mcs2020
  • Kelly, T. D., Matos, G. R., Buckingham, D. A., DiFrancesco, C. A., Porter, K. E., & Berry, C. (2010). Historical statistics for mineral and material commodities in the United States. US Geological Survey Data Series, 140, 01-006.
  • Mohr, S. H., Mudd, G. M., & Giurco, D. (2012). Lithium resources and production: critical assessment and global projections. Minerals, 2(1), 65-84. https://doi.org/10.3390/min2010065
  • Avila-Arias, H., Nies, L. F., Gray, M. B., & Turco, R. F. (2019). Impacts of molybdenum-, nickel-, and lithium-oxide nanomaterials on soil activity and microbial community structure. Science of the Total Environment, 652, 202-211. https://doi.org/10.1016/j.scitotenv.2018.10.189
  • Zeng, X., & Li, J. (2014). Spent rechargeable lithium batteries in e-waste: composition and its implications. Frontiers of Environmental Science and Engineering, 8, 792-796, https://doi.org/10.1007/s11783-014-0705-6
  • Person, E. C., Meyer, J. A., & Vyvyan, J. R. (2005). Structural determination of the principal byproduct of the lithium-ammonia reduction method of methamphetamine manufacture. Journal of Forensic Sciences, 50(1), JFS2004204-9. https://doi.org/10.1520/JFS2004204
  • Mahgoub, M. O., Lau, W., & Omar, D. (2015). Observations on the biology and larval instars discrimination of wax moth Achroia grisella F. (Pyralidae: Lepidoptera). Journal of Entomology, 12(1), 1-11. https://doi.org/10.3923/je.2015.1.11
  • Williams, J. L. (1997). Insects: Lepidoptera (moths). Honey bee pests, predators, and diseases, 3, 119-142.
  • Çelik, D., Özbek, R., & Uçkan, F. (2017). Effects of indole-3-acetic acid on hemocytes of Achoria grisella Fabr. (Lepidoptera: Pyralidae). Journal of the Entomological Research Society, 19(2), 83-93.
  • Gündüz, N. E. A., Mercan, S., & Özcan, Ö. (2020). Effect of cadmium and lead on total hemocyte count of Achroia grisella Fabr. (Lepidoptera: Pyralidae). Gümüşhane Üniversitesi Fen Bilimleri Dergisi, 10(1), 190-194. https://doi.org/10.17714/gumusfenbil.531102
  • Akman Gündüz, N. E., & Yılmaz, Y. (2022). Kurşun ile kontamine edilen Achroia grisella Fabr. (Lepidoptera: Pyralidae) larvalarının protein, lipit ve karbohidrat kompozisyonundaki değişiklikler. Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi, 23(1), 154-159. https://doi.org/10.17474/artvinofd.1021280
  • Prđun, S., & Virić Gašparić, H. (2023). Veliki (Galleria mellonella L.) i mali (Achroia grisella Fabricius) voskov moljac–štetnici u pčelarstvu i metode suzbijanja. Glasilo Biljne Zaštite, 23(3), 360-373. https://urn.nsk.hr/urn:nbn:hr:204:157540
  • Sak O, Uçkan F & Ergin, E (2006). Effects of cypermethrin on total body weight, glycogen,protein and lipid contents of Pimpla turionella (L.) (Hymenoptera: Ichneumonidae). Belgian Journal of Zoology, 136(1), 53-58.
  • Bronskill, J. (1961). A cage to simplify the rearing of the greater wax moth, Galleria mellonella (Pyralidae). Journal of the Lepidopterists' Society, 15(2), 102-104.
  • Lowry, O.H., Rosebrough, N.J., Farr, A.L., & Randall, V.J., (1951). Protein measurement with the Folin Phenol Reagent. Journal of Biological Chemistry, 193, 265-275.
  • Olson, D. A. W. N. M., Fadamiro, H., Lundgren, J. N. G., & Heimpel, G. E. (2000). Effects of sugar feeding on carbohydrate and lipid metabolism in a parasitoid wasp. Physiological Entomology, 25(1), 17-26. https://doi.org/10.1046/j.1365-3032.2000.00155.x
  • Fadamiro, H.Y., & Heimpel, G.E., (2001). Effects of partial Sugar deprivation on lifespan and carbohydrate mobilization in the parasitoid Macrocentrus grandii (Hymenoptera: Braconidae). Annals of Entomological Society of America, 94(6), 909-916. https://doi.org/10.1603/0013-8746(2001)094[0909: EOPSDO]2.0.CO;2
  • Van Handel, E. (1985b). Rapid determination of total lipids in mosquitoes. Journal of the American Mosquito Control Association, 1(3), 302-304.
  • Van Handel, E., (1985a). Rapid determination of glycogen and sugars in mosquitoes. Journal of the American Mosquito Control Association, 1(3), 299-301.
  • Emre, I., Kayış, T., Coskun, M., Dursun, O., & Cogun, H. Y. (2013). Changes in antioxidative enzyme activity, glycogen, lipid, protein, and malondialdehyde content in cadmium-treated Galleria mellonella larvae. Annals of the Entomological Society of America, 106(3), 371-377. https://doi.org/10.1603/AN12137
  • Islam, A., & Roy, S. (1983). Effect of CdCl2 on the quantitative variation of carbohydrate, protein, amino acids and cholesterol in Chrysocoris stollii Wolf (Insecta: Hemiptera). Current Science, 52(5), 215-217. http://www.jstor.org/stable/24085483
  • Ortel, J. (1991). Effects of lead and cadmium on chemical composition and total water content of the pupal parasitoid, Pimpla turionellae. Entomologia Experimentalis et Applicata, 59(1), 93-100. https://doi.org/10.1111/j.1570-7458.1991.tb01491.x
  • Wu, G. X., Ye, G. Y., Hu, C. U. I., & Cheng, J. A. (2006). Accumulation of cadmium and its effects on growth, development and hemolymph biochemical compositions in Boettcherisca peregrina larvae (Diptera: Sarcophagidae). Insect Science, 13(1), 31-39, https://doi.org/10.1111/j.1744-7917.2006.00065.x
  • Norton, K. B., & Kench, J. E. (1977). Effects of cadmium on ribosomal protein synthesis in rat liver. Environmental Research, 13(1), 102-110. https://doi.org/10.1016/0013-9351(77)90008-1
  • Sancho, E., Ferrando, M. D., Fernandez, C., & Andreu, E. (1998). Liver energy metabolism of Anguilla anguilla after exposure to fenitrothion. Ecotoxicology and Environmental Safety, 41(2), 168-175. https://doi.org/10.1006/eesa.1998.1689
  • Baghban, A., Sendi, J. J., Khosravi, R., & Zibaee, A. (2014). Effect of heavy metals (Cd, Cu, and Zn) on feeding indices and energy reserves of the cotton boll worm Helicoverpa armigera Hübner (Lepidoptera: Noctuidae). Journal of Plant Protection Research, 54(4), 368-371. https://doi.org/10.2478/jppr-2014-0055
  • El-Sheikh, E. S. M., Fouda, M. A., Hassan, M. I., Abd-Elghaphar, A. E. A., & Hasaballah, A. I. (2010). Toxicological effects of some heavy metal ions on Culex pipiens L. (Diptera: Culicidae). Egyptian Academic Journal of Biological Sciences, F. Toxicology and Pest Control, 2(1), 63-76. https://doi.org/10.21608/eajbsf.2010.17465
  • Shin, B. S., Choi, R. N., & Lee, C. U. (2001). Effects of cadmium on total lipid content and fatty acids of the greater wax moth, Galleria mellonella. The Korean Journal of Ecology, 24(6), 349-352.
  • Bischof, C. (1995). Effects of heavy metal stress on carbohydrate and lipid concentrations in the haemolymph and total body tissue of parasitized Lymantria dispar L. larvae (Lepidoptera). Comparative Biochemistry and Physiology Part C: Pharmacology, Toxicology and Endocrinology, 112(1), 87-92. https://doi.org/10.1016/0742-8413(95)00079-8
  • Kayış, T., & Emre, I. (2012). Effects of heavy metal stress on protein and glycogen synthesis of Pimpla turionellae (Hymenoptera: Ichneumonidae). Ekoloji, 21(83), 61-67.

Influence of Lithium on Protein, Lipid and Carbohydrate Level of $Achroia$ $grisella$ Fabr. (Lepidoptera: Pyralidae) Larvae

Yıl 2025, Cilt: 10 Sayı: 2, 401 - 409, 24.12.2025
https://doi.org/10.33484/sinopfbd.1651126

Öz

In this study, the influence of lithium (Li) on protein, lipid and carbohydrate concentrations of Lesser Wax Moth larvae was examined. Larvae were reared on an artificial diet contaminated with different concentrations of lithium (125, 250 and 500 mg/kg) and last instar larvae were used for biochemical analysis. All insects were kept at 25 ± 2 °C temperature, 25 ± 2 °C RH and 16L:8D photoperiod conditions. Treatment with lithium decreased the protein levels of larvae at 125 and 500 mg/kg concentrations whereas lipid levels of larvae significantly increased at the same lithium concentrations. However, lithium did not make any significant change in carbohydrate level of the larvae.

Proje Numarası

BAP04-A-2023-4752

Kaynakça

  • Tanveer, M., Hasanuzzaman, M., & Wang, L. (2019). Lithium in environment and potential targets to reduce lithium toxicity in plants. Journal of Plant Growth Regulation, 38(4), 1574-1586. https://doi.org/10.1007/s00344-019-09957-2
  • Aral, H., & Vecchio-Sadus, A. (2008). Toxicity of lithium to humans and the environment—a literature review. Ecotoxicology and Environmental Safety, 70(3), 349-356. https://doi.org/10.1016/j.ecoenv.2008.02.026
  • US Geological Survey. (2020). Mineral commodity summaries 2020. US Geological Survey, 200p. https://doi.org/10.3133/mcs2020
  • Kelly, T. D., Matos, G. R., Buckingham, D. A., DiFrancesco, C. A., Porter, K. E., & Berry, C. (2010). Historical statistics for mineral and material commodities in the United States. US Geological Survey Data Series, 140, 01-006.
  • Mohr, S. H., Mudd, G. M., & Giurco, D. (2012). Lithium resources and production: critical assessment and global projections. Minerals, 2(1), 65-84. https://doi.org/10.3390/min2010065
  • Avila-Arias, H., Nies, L. F., Gray, M. B., & Turco, R. F. (2019). Impacts of molybdenum-, nickel-, and lithium-oxide nanomaterials on soil activity and microbial community structure. Science of the Total Environment, 652, 202-211. https://doi.org/10.1016/j.scitotenv.2018.10.189
  • Zeng, X., & Li, J. (2014). Spent rechargeable lithium batteries in e-waste: composition and its implications. Frontiers of Environmental Science and Engineering, 8, 792-796, https://doi.org/10.1007/s11783-014-0705-6
  • Person, E. C., Meyer, J. A., & Vyvyan, J. R. (2005). Structural determination of the principal byproduct of the lithium-ammonia reduction method of methamphetamine manufacture. Journal of Forensic Sciences, 50(1), JFS2004204-9. https://doi.org/10.1520/JFS2004204
  • Mahgoub, M. O., Lau, W., & Omar, D. (2015). Observations on the biology and larval instars discrimination of wax moth Achroia grisella F. (Pyralidae: Lepidoptera). Journal of Entomology, 12(1), 1-11. https://doi.org/10.3923/je.2015.1.11
  • Williams, J. L. (1997). Insects: Lepidoptera (moths). Honey bee pests, predators, and diseases, 3, 119-142.
  • Çelik, D., Özbek, R., & Uçkan, F. (2017). Effects of indole-3-acetic acid on hemocytes of Achoria grisella Fabr. (Lepidoptera: Pyralidae). Journal of the Entomological Research Society, 19(2), 83-93.
  • Gündüz, N. E. A., Mercan, S., & Özcan, Ö. (2020). Effect of cadmium and lead on total hemocyte count of Achroia grisella Fabr. (Lepidoptera: Pyralidae). Gümüşhane Üniversitesi Fen Bilimleri Dergisi, 10(1), 190-194. https://doi.org/10.17714/gumusfenbil.531102
  • Akman Gündüz, N. E., & Yılmaz, Y. (2022). Kurşun ile kontamine edilen Achroia grisella Fabr. (Lepidoptera: Pyralidae) larvalarının protein, lipit ve karbohidrat kompozisyonundaki değişiklikler. Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi, 23(1), 154-159. https://doi.org/10.17474/artvinofd.1021280
  • Prđun, S., & Virić Gašparić, H. (2023). Veliki (Galleria mellonella L.) i mali (Achroia grisella Fabricius) voskov moljac–štetnici u pčelarstvu i metode suzbijanja. Glasilo Biljne Zaštite, 23(3), 360-373. https://urn.nsk.hr/urn:nbn:hr:204:157540
  • Sak O, Uçkan F & Ergin, E (2006). Effects of cypermethrin on total body weight, glycogen,protein and lipid contents of Pimpla turionella (L.) (Hymenoptera: Ichneumonidae). Belgian Journal of Zoology, 136(1), 53-58.
  • Bronskill, J. (1961). A cage to simplify the rearing of the greater wax moth, Galleria mellonella (Pyralidae). Journal of the Lepidopterists' Society, 15(2), 102-104.
  • Lowry, O.H., Rosebrough, N.J., Farr, A.L., & Randall, V.J., (1951). Protein measurement with the Folin Phenol Reagent. Journal of Biological Chemistry, 193, 265-275.
  • Olson, D. A. W. N. M., Fadamiro, H., Lundgren, J. N. G., & Heimpel, G. E. (2000). Effects of sugar feeding on carbohydrate and lipid metabolism in a parasitoid wasp. Physiological Entomology, 25(1), 17-26. https://doi.org/10.1046/j.1365-3032.2000.00155.x
  • Fadamiro, H.Y., & Heimpel, G.E., (2001). Effects of partial Sugar deprivation on lifespan and carbohydrate mobilization in the parasitoid Macrocentrus grandii (Hymenoptera: Braconidae). Annals of Entomological Society of America, 94(6), 909-916. https://doi.org/10.1603/0013-8746(2001)094[0909: EOPSDO]2.0.CO;2
  • Van Handel, E. (1985b). Rapid determination of total lipids in mosquitoes. Journal of the American Mosquito Control Association, 1(3), 302-304.
  • Van Handel, E., (1985a). Rapid determination of glycogen and sugars in mosquitoes. Journal of the American Mosquito Control Association, 1(3), 299-301.
  • Emre, I., Kayış, T., Coskun, M., Dursun, O., & Cogun, H. Y. (2013). Changes in antioxidative enzyme activity, glycogen, lipid, protein, and malondialdehyde content in cadmium-treated Galleria mellonella larvae. Annals of the Entomological Society of America, 106(3), 371-377. https://doi.org/10.1603/AN12137
  • Islam, A., & Roy, S. (1983). Effect of CdCl2 on the quantitative variation of carbohydrate, protein, amino acids and cholesterol in Chrysocoris stollii Wolf (Insecta: Hemiptera). Current Science, 52(5), 215-217. http://www.jstor.org/stable/24085483
  • Ortel, J. (1991). Effects of lead and cadmium on chemical composition and total water content of the pupal parasitoid, Pimpla turionellae. Entomologia Experimentalis et Applicata, 59(1), 93-100. https://doi.org/10.1111/j.1570-7458.1991.tb01491.x
  • Wu, G. X., Ye, G. Y., Hu, C. U. I., & Cheng, J. A. (2006). Accumulation of cadmium and its effects on growth, development and hemolymph biochemical compositions in Boettcherisca peregrina larvae (Diptera: Sarcophagidae). Insect Science, 13(1), 31-39, https://doi.org/10.1111/j.1744-7917.2006.00065.x
  • Norton, K. B., & Kench, J. E. (1977). Effects of cadmium on ribosomal protein synthesis in rat liver. Environmental Research, 13(1), 102-110. https://doi.org/10.1016/0013-9351(77)90008-1
  • Sancho, E., Ferrando, M. D., Fernandez, C., & Andreu, E. (1998). Liver energy metabolism of Anguilla anguilla after exposure to fenitrothion. Ecotoxicology and Environmental Safety, 41(2), 168-175. https://doi.org/10.1006/eesa.1998.1689
  • Baghban, A., Sendi, J. J., Khosravi, R., & Zibaee, A. (2014). Effect of heavy metals (Cd, Cu, and Zn) on feeding indices and energy reserves of the cotton boll worm Helicoverpa armigera Hübner (Lepidoptera: Noctuidae). Journal of Plant Protection Research, 54(4), 368-371. https://doi.org/10.2478/jppr-2014-0055
  • El-Sheikh, E. S. M., Fouda, M. A., Hassan, M. I., Abd-Elghaphar, A. E. A., & Hasaballah, A. I. (2010). Toxicological effects of some heavy metal ions on Culex pipiens L. (Diptera: Culicidae). Egyptian Academic Journal of Biological Sciences, F. Toxicology and Pest Control, 2(1), 63-76. https://doi.org/10.21608/eajbsf.2010.17465
  • Shin, B. S., Choi, R. N., & Lee, C. U. (2001). Effects of cadmium on total lipid content and fatty acids of the greater wax moth, Galleria mellonella. The Korean Journal of Ecology, 24(6), 349-352.
  • Bischof, C. (1995). Effects of heavy metal stress on carbohydrate and lipid concentrations in the haemolymph and total body tissue of parasitized Lymantria dispar L. larvae (Lepidoptera). Comparative Biochemistry and Physiology Part C: Pharmacology, Toxicology and Endocrinology, 112(1), 87-92. https://doi.org/10.1016/0742-8413(95)00079-8
  • Kayış, T., & Emre, I. (2012). Effects of heavy metal stress on protein and glycogen synthesis of Pimpla turionellae (Hymenoptera: Ichneumonidae). Ekoloji, 21(83), 61-67.
Toplam 32 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Konular Hayvan Bilimi (Diğer)
Bölüm Araştırma Makalesi
Yazarlar

Nevran Eylem Akman Gündüz 0000-0001-5777-470X

Zeynep Çevik 0009-0002-8890-5711

Proje Numarası BAP04-A-2023-4752
Gönderilme Tarihi 5 Mart 2025
Kabul Tarihi 18 Ağustos 2025
Yayımlanma Tarihi 24 Aralık 2025
Yayımlandığı Sayı Yıl 2025 Cilt: 10 Sayı: 2

Kaynak Göster

APA Akman Gündüz, N. E., & Çevik, Z. (2025). Lityumun $Achroia$ $grisella$ Fabr. (Lepidoptera: Pyralidae) Larvalarının Protein, Lipit ve Karbohı̇drat Miktarına Etkı̇sı̇. Sinop Üniversitesi Fen Bilimleri Dergisi, 10(2), 401-409. https://doi.org/10.33484/sinopfbd.1651126


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