Morphological, molecular and toxicologial characterization of Nodularia spumigena Mertens in Jungens (1822) from brackishwater Lake Bafa (Turkey)

Öz

Nodularia spumigena, a bloom-forming cyanobacteria, can produce hepatotoxic peptide nodularin. Here morphological, molecular and chemical methods were applied to characterize toxic and bloom-forming N. spumigena collected from Lake Bafa on June 2010, Aydın, Turkey. Phylogenetic analysis of N. spumigena was performed by sequencing 16S ribosomal DNA or the phycocyanin intergenic spacer (IGS) region, and the nodularin synthetase gene cluster (nda). Nodularin produced by N. spumigena was determined by using enzyme-linked immunosorbent assay (ELISA), high performance liquid chromatography (HPLC) and liquid chromatography/mass spectrometry (LC/MS). To our knowledge, this is the first report on toxic N. spumigena in Lake Bafa, Turkey.

Anahtar Kelimeler

• lake Bafa,cyanobacteria,HPLC,nodularin,ELISA

Bafa Gölü'ndeki Nodularia spumigena Mertens in Jungens (1822)'in morfolojik, moleküler ve toksikolojik karakterizasyonu

Öz

Aşırı alg çoğalması oluşturan Nodularia spumigena, hepatotoksik peptid nodularini üretir.  Bu çalışmada Haziran ayında Bafa Gölünden izole edilen N. spumigena’nın karakterizasyonu için gerçekleştirilen morfolojik, moleküler ve kimyasal  analizler uygulanmıştır. Filogenetik analizler 16S rDNA, fikosiyanin intergenik spacer (IGS) bölgesi ve nodularin sentez gen kümesi (nda) çoğaltılarak gerçekleştirilmiştir.

N. spumigena’nın ürettiği nodularinin tayini  enzyme-linked immunosorbent assay (ELISA), high performance liquid chromatography (HPLC) ve liquid chromatography/mass spectrometry (LC/MS) yöntemleriyle araştırılmıştır.

Bu çalışma Bafa Gölü’nde toksik N. spumigena’nın ilk karakterizasyonudur.

Anahtar Kelimeler

• Bafa Gölü,HPLC,ELISA,nodularin,siyanobakteri

Kaynakça

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