High SNAIL Expression Reflects Of Recurrence After Resection Colorectal Cancer Liver Metastasis

Abstract

Introduction: Metastasectomy is one of the effective treatment methods for liver metastases developing from primary colorectal cancer (CRC). However, recurrence after metastasectomy is the biggest problem in these cancers. The present study aimed to examine the role of Epithelial-Mesenchymal Transition (EMT) in the development of recurrence after metastasectomy in liver metastases from primary CRCs. Materials and Methods: In the study, 85 patients were evaluated. RNA was isolated from archive tumors and normal tissues of the patients. Expression profiles of 7 different EMT markers were examined using qRT-PCR. Results: Metastasis to the liver developed in 24.7% (n=21) of 85 CRC. Recurrence was observed in 42.3% (n=9) of liver metastases after metastasectomy. Statistically significant increases were detected in the expression of EMT markers TWIST and SNAIL in the development of mCC in primary CRC (p<0.05). On the other hand, in recurrent mCCs after metastasectomy, only SNAIL expression, one of the EMT markers, showed a statistically significant increase in the relapsed group compared to non-relapsed liver tissues (p<0.0001). Conclusion: As a result of the study, it is predicted that primary CRC tumors with high SNAIL expression will develop mCC within the follow-up period, and recurrence may occur after metastasectomy in these cases.

Keywords

• Metastasectomy
• metastatic liver cancers
• Epithelial-Mesenchymal Transition

Yüksek Snail ekspresyonu Kolorektal Kanser Kökenli Karaciğer Metaztaslarında Rezeksiyon Sonrası Nüksü Yansıtır

Abstract

Amaç: Cerrahi tedavi, primer kolorektal kanserden (KRK) gelişen karaciğer metastazları için etkili tedavi yöntemlerinden biridir. Bununla birlikte metastazektomi sonrası nüks bu kanserlerdeki en büyük problemdir. Mevcut çalışmada, primer KRK’lardan gelişen karaciğer metastazlarında Epitelyal-Mezenkimal Tranzisyon (EMT)’nin metastazektomi sonrası nüks gelişimindeki rolünün incelenmesi amaçlanmıştır. Gereç ve Yöntemler: Çalışmada, 85 hasta, 12 normal kolon, 8 normal karaciğer dokusu değerlendirildi. Hastalara ait arşiv materyali tümör ve normal dokularından RNA izolasyonları yapıldı. qRT-PCR kullanılarak 7 farklı EMT belirtecinin ekspresyon profili incelendi. Bulgular: Seksen beş KRK’nın %24.7 (n=21)’sinde karaciğere metastazı gelişti. Karaciğer metastazı belirlenen hastaların %42.3’ünde (n=9) metastazektomi sonrası nüks gözlendi. Primer KRK’dan mKK gelişiminde EMT belirteçlerinden TWIST ve SNAIL ekspresyonlarında istatistiksel olarak anlamlı artış belirlendi (p<0.05). Cerrahi tedavi sonrası nüks eden mKK’larda ise EMT belirteçlerinden sadece SNAIL ekspresyonunda nüks etmeyen karaciğer dokularına göre nüks eden grupta istatistiksel olarak anlamlı artış gösterdi (p<0.0001). Sonuç: Çalışma sonucunda, yüksek SNAIL ekspresyonuna sahip primer KRK tümörlerinin takip süresi içerisinde mKK geliştireceğini ve bu vakalara metastazektomi yapılmasına rağmen sonrasında nüks oluşabileceği öngörülmektedir.

Keywords

• Metastazektomi
• metastatik karaciğer kanserleri
• Epitelyal-Mezenkimal Tranzisyon

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