Protective Effect of p-Coumaric Acid as Free Oxygen Radical Scavenger in Experimental Renal Ischemia-Reperfusion Model

Yıl 2018, , 625 - 631, 30.09.2018

Derya Güzel , Ayhan Tanyeli

https://doi.org/10.31832/smj.455724

Cited By: 1

Öz

 

ÖZET

Amaç: Bu
çalışmanın amacı, sıçan böbrek dokusunda iskemi-reperfüzyona bağlı doku hasarı
üzerine p-kumarik asit (p-CA)’in antioksidan etkisini değerlendirmektir.

Gereç ve Yöntemler: 12-16 haftalık 200-250 gr otuz
iki adet Wistar Albino cinsi dişi sıçan temin edildi. Sıçanlara intraperitonal olarak
ketamine (75 mg/kg) ve xylazine (8 mg/kg) anestezisi altında sırt bölgesinden
yapılan bir kesi ile sağ nefrektomi uygulandı. Bu sıçanlar randomize olarak
dört gruba eşit olarak bölündü (n=8). Gruplar; sham (S), renal iskemi-reperfüzyon
(R-IR), R-IR ile 50 mg/kg p-kumarik asit (p-CA 50) ve R-IR ile 100 mg/kg p-kumarik
asit (p-CA 100) uygulanan grup olarak sınıflandırıldı. Sham hariç diğer
gruplardaki sıçanların sırt bölgesi açılıp sağ nefrektomi yapıldıktan sonra sol
renal arter klemplenerek  iskemi ve
reperfüzyon protokolü uygulandı. P-kumarik asit iskemiden 60 dk önce ve
reperfüzyon başlangıcından 30 dk önce olmak üzere iki doz şeklinde uygulandı.
İşlemler tamamlandıktan sonra sol böbrek total antioksidan düzeyi (TAS), total
oksidan düzeyi (TOS), süperoksit dismutaz (SOD), malondialdehit (MDA) ve
myeloperoksidaz (MPO) seviyeleri spektrofotometrik yöntemlerle ölçüldü.
Oksidatif stres indeksi (OSI) hesaplandı.

Bulgular: R-IR grubunda S ile karşılaştırıldığında TAS
ve SOD değeri düşerken, TOS, MDA, MPO ve OSI değerleri yükseldi (p<0.01). P-CA
uygulanan gruplarda TAS ve SOD değerinde yükselme, TOS, MDA ve MPO değerlerinde
ise düşme gözlendi. (p<0.05).

Sonuç: Bulgularımız, renal iskemi
reperfüzyon hasarında p-CA uygulamasının oksidan hasarı azaltarak dokunun
korunmasında rol oynayabileceğini göstermektedir.  

Anahtar Kelimeler

p-coumaric acid, renal ischemia-reperfusion injury, oxidative stress.

Kaynakça

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