Polymorphism and CDC25A Gene Expression in Pancreatic Cancer

Yıl 2016, Cilt: 6 Sayı: 1, 7 - 13, 14.03.2016

Başar Aksoy Sacit Çoban Serdar Öztuzcu Erdal Uysal

Öz

Background and Aim: The aim of this study is to determine the relationship between CDC25A gene polymorphism and pancreatic cancer via determining
CDC25A gene expression and polymorphisms of tumor tissues of pancreatic cancer cases. Additionally, it is aimed to prove the
possible relationship between cancer development and the gene expression level with determining CDC25A gene expression
levels in tumor tissue and normal tissue.
Materials and Methods: 118 patients (patients with pancreatic cancer who received surgery (n=28) and patients with pancreatic cancer who are in followup (n=90) and 83 healthy volunteers not having any known chronic disease and first degree relatives having cancer were included
in this study as a control group.
Results: Ser88Phee polymorphisms in CDC25A gene of pancreatic cancer and control groups were compared according to C/C, C/T, T/T
genotype frequencies and allele frequencies of C and T and no statistically significant difference was detected between two
groups (p>0.05). RS3731485 polymorphisms in CDC25A gene of pancreatic cancer and control groups were compared with
respect to C/C, C/G, G/G genotype frequencies and allele frequencies of C and G and there was no significant difference between
them statistically (p>0.05). There were no differences in CDC25A gene expression between control group and pancreatic cancer
group (p>0.05).
Conclusion: In the light of the data obtained, any significant relationship at the CDC25A gene polymorphism and expression in pancreatic
cancer was not detected. All in all, pancreatic cancer is a disease with high mortality and the place of genetics in the etiology of
cancer is indisputable. Therefore, we think the polymorphism and expression studies should be continued.. 

Anahtar Kelimeler

Pancreatic cancer, CDC25A, polymorphism, expression

Pankreas Kanserindeki CD25A Gen Ekspresyonu ve Polimorfizmi

Öz

Amaç: Bu çalışmanın amacı pankreas kanser vakalarının tümör dokularının CDC25A gen ekspiresyonu ve polimorfizmi belirlenmesi yoluyla CD25A gen polimorfizmi ve pankreas kanseri arasındaki ilişkinin belirlenmesi amaçlanmıştır.Ek olarak kanser gelişimi ve gen
ekspiresyon seviyesi ile normal doku ve tümör dokusundaki CD25A gen ekspiresyonu arasındaki ilişkinin ihtimali amaçlanmıştır. .
Materyal ve Metod: 118 hasta (pankreas kanseri nedeniyle ameliyat olan (n=28) hasta ve pankreas kanseri nedeniyle takip edilen (n=90) hasta, birinci
derece akrabalarında kanser olan 83 sağlıklı gönüllü çalışmada kontrol grubu olarak çalışmaya dahil edildi.
Bulgular: Pankreas kanserinin ve kontrol gurubunun CD25A genindeki Ser88Phee polimorfizmi, C/C, C/T, T/T genotip sıklığı ve C ve T’nin
alel sıklığı arasında yapılan karşılaştırmada iki grup arasında istatistiksel anlmalılık bulunmadı (p>0.05). Pankreas kanserinin ve
kontrol gurubunun CD25A genindeki RS3731485 polimorfizmi, C/C, C/G, G/G, genotip sıklığı ve C ve G’nin alel sıklığı arasında
yapılan karşılaştırmada iki grup arasında istatistiksel anlamlılık bulunmadı (p>0.05). pankreas kanser ve kontrol grupları arasında
gen ekspiresyonu açısından anlamlılık yoktu .
Sonuç: Elde edilen veriler ışığında pankreas kanserinde CD25A gen polimorfizmi ve ekspiresyonu ile hiçbir anlamlı ilişki tespit edilmedi.
Sonuçta pankreas kanseri yüksek mortaliteye sahip bir hastalıktır ve kanserin etiyolojisinde genetiğin yeri tartışılamaz. Bu yüzden
polimorfizim ve ekspirasyon çalışmalarına devam edilmesini düşünüyoruz

Anahtar Kelimeler

Pankreas kanseri, CDC25A, Polimorfizim, Ekspiresyon

Kaynakça

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