Deneysel Renal İskemi Reperfüzyon Modelinde Akciğer Dokusunda Oksidan Hasarın İncelenmesi ve Klorojenik Asit (CGA) Kullanımının Koruyucu Etkileri

Yıl 2018, Cilt: 8 Sayı: 2, 260 - 265, 29.06.2018

Derya Güzel , Ayhan Tanyeli

https://doi.org/10.31832/smj.423683

Öz

Amaç: Bu
çalışmanın amacı, sıçan akciğer dokusunda iskemi-reperfüzyona bağlı doku hasarı
üzerine Klorojenik Asit (CGA) etkisini değerlendirmektir.

Gereç ve Yöntemler: Otuz iki adet Wistar Albino
cinsi dişi sıçan temin edildi. Bu sıçanlar randomize olarak dört gruba eşit
olarak bölündü (n=8). Oluşturulan gruplar; sham (S), iskemi-reperfüzyon (IR), 5
mg/kg Klorojenik Asit (CGA5) ve 10 mg/kg Klorojenik Asit (CGA10) uygulanan grup
olarak tanımlanarak cerrahi işlemlere tabi tutuldu. Sham grubundaki sıçanlar
sırt bölgesinden açılarak sağ nefrektomi yapıldı. Sham hariç diğer gruplardaki
sıçanların sırt bölgesi açılıp sağ nefrektomi yapıldıktan sonra sol renal arter
klemplendi. Klorojenik asit iskemiden 10 dk önce ve reperfüzyondan 10 dk önce
olmak üzere iki doz şeklinde intraperitoneal olarak uygulandı. Sol böbreğe 1
saatlik iskemiden sonra 24 saatlik reperfüzyon uygulandı. İşlemler
tamamlandıktan sonra akciğer dokularında total antioksidan düzeyi (TAS), total
oksidan düzeyi (TOS), süperoksit dismutaz (SOD), malondialdehit (MDA) ve
myeloperoksidaz (MPO) seviyeleri spektrofotometrik yöntemlerle ölçüldü.
Oksidatif stres indeksi (OSI) hesaplandı.

Bulgular: IR grubunda S ile karşılaştırıldığında TAS ve SOD değeri
düşerken, TOS, MDA ve MPO değerleri yükseldi. CGA uygulanan gruplarda TAS ve
SOD değerinde yükselme, TOS, MDA ve MPO değerlerinde ise düşme gözlendi.

Sonuç: CGA tedavisinin renal iskemi reperfüzyona bağlı akciğerde
gelişen oksidatif stres hasarının azaltılmasında etkili olabileceği
düşünülmektedir.

Anahtar Kelimeler

Klorojenik asit, renal iskemi reperfüzyon, akciğer.

Kaynakça

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