DETERMINATION OF THE GENETIC PROFILE OF MCT1 (SLC16A1) GENE A/T POLYMORPHISM AND GDF5 GENE T/C POLYMORPHISM RELATED TO CONNECTIVE TISSUE AND MUSCLE INJURY RISK IN YOUNG ATHLETES

Öz

In this study, MCT1 (SLC16A1) gene A/T polymorphism related to muscle injury and GDF5 T/C polymorphism related to connective tissue damage in young athletes were studied and it was investigated whether there was a difference in allele distribution in the athlete group related to connective tissue and muscle injury compared to the control group. Whether the allele distributions of the experimental and control groups were in balance was tested using the Hardy-Weinberg equation. Differences between groups were analyzed with Chi square Test. The confidence interval was determined as 95% (p<0.05). As a result of the research, no significant difference was found between the control group and athletes in terms of SLC16A1 gene A/T polymorphism (rs1049434). A significant difference was found between the athletes and the control group in terms of GDF5 gene T/C polymorphism (rs143383). CC and TC genotypes in athletes were more common than TT genotypes in individuals in the control group. This situation is in favor of athletes. As a result of the genotypes determined by studying the SLC16A1 and GDF5 gene polymorphisms in elite athlete groups within the scope of the research, coaches and club sports physicians can be informed and strategies can be developed to plan training to prevent injuries and to intervene in case of possible injury.

Anahtar Kelimeler

• Gene
• Polymorphism
• Injury
• SLC16A1 (MCT1)
• GDF5

GENÇ ATLETLERDE BAĞ DOKUSU VE KAS YARALANMA RİSKİ İLE İLGİLİ MCT1 (SLC16A1) GENİ A/T POLİMORFİZMİ VE GDF5 GENİ T/C POLİMORFİZMİNE AİT GENETİK PROFİLİN BELİRLENMESİ

Öz

Bu araştırmada, genç atletlerde kas yaralanması ile ilgili MCT1 (SLC16A1) geni A/T polimorfizmi ve bağ doku hasarı ile ilgili GDF5 T/C polimorfizmi çalışılarak bağ doku ve kas yaralanması ile ilgili sporcu grubunda kontrol grubuna göre alel dağılımında bir farklılık bulunup bulunmadığı araştırılmıştır. Deney ve kontrol grubuna ait alel dağılımlarının dengede olup olmadığı Hardy-Weinberg eşitliği kullanılarak test edilmiştir. Gruplar arasındaki farklılık Chi square Testi ile analiz edilmiştir. Güven aralığı %95 olarak belirlenmiştir (p<0,05). Araştırmanın sonucunda SLC16A1 geni A/T polimorfizmi (rs1049434) açısından kontrol grubu ve sporcular arasında anlamlı bir fark bulunmamıştır. GDF5 geni T/C polimorfizmi (rs143383) açısından sporcular ile kontrol grubu arasında anlamlı bir fark bulunmuştur. Sporculardaki CC ve TC genotipi kontrol grubunda bulunan bireylerdeki TT genotipinden daha sık görülmüştür. Bu durum sporcuların lehinedir. Araştırma kapsamında çalışılan SLC16A1 ve GDF5 gen polimorfizmleri elit sporcu gruplarında çalışılarak belirlenen genotipler sonucunda antrenörler ve kulüp spor hekimleri bilgilendirilip yaralanmanın engellenmesine yönelik antrenmanlar planlanılması ve olası yaralanma durumunda müdahale için stratejiler geliştirilebilir.

Anahtar Kelimeler

• Gen
• Polimorfizm
• Yaralanma
• SLC16A1 (MCT1)
• GDF5

Etik Beyan

Deney ve kontrol gruplarının seçiminde etik kurallara uyulmuştur. Grup seçimi sırasında kan örnekleri alınacak olduğundan reşit olmayan sporcu ve kontrol grubu için Ebeveyn Onam Formu ve Çocuk Onam Formu hazırlanarak hem ailelerden hem de sporcu ve kontrol grubunu oluşturan çocuklardan izin alındı. Araştırma için Gümüşhane Üniversitesi Etik Kurulu'ndan onay alındı (Karar No: E-95674917-108.99-101079, Karar Tarihi: 22/06/2022). Lisanslı sporcularla çalışmak için Gümüşhane Gençlik ve Spor İl Müdürlüğü'nden izin alındı.

Teşekkür

Araştırmamıza katılan tüm kişilere ve antrenörlere teşekkür ederiz. Genetik analizlerimizi gerçekleştirdiğimiz Genoks Genetik Hastalıklar Değerlendirme Merkezi'ne de teşekkür ederiz.

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