Yıl 2019, Cilt 1 , Sayı 1, Sayfalar 8 - 14 2019-08-30

Genotoxic and Cytotoxic Activity of Cyanobacterial (Blue-Green Algal) Toxin BMAA in Human Lymphocyte Cells

Arif AYAR [1] , Deniz ALTUN COLAK [2] , Handan UYSAL [3] , Vahit KONAR [4]


Cyanobacteria, one of the oldest life forms, are also known as blue-green algae. Many species of cyanobacteria produce metabolites called cyanotoxin, which are classified as hepatotoxins, neurotoxins and cytotoxins and are highly toxic to vertebrate organisms. One of these toxins is the Beta-N-methyl-amino-L-alanine (BMAA). Blooms (large numbers or colonies) of the cyanobacteria or related organisms produce one or more toxins that can be dangerous to fish, wild animals and humans. There is not enough information in the literature related to the genotoxic effect of this toxin. In this study, it was aimed to determine investigation of the cytotoxic and genotoxic activity of cyanobacterial toxin BMAA in human lymphocyte cells by micronucleus assay. Different concentrations (20, 10, 5 and 1 μg/ml) of BMAA cyanotoxin were applied to human lymphocyte cell culture and micronucleus frequencies (MN) and nuclear division index (NDI) were calculated to determine genotoxic and cytotoxic effects. The data obtained from our study were compared with the negative control group prepared with dimethyl sulfoxide (DMSO) and with the well known ethyl methanesulfonate (EMS) with genotoxic effect and positive control group. According to these results; increasing the frequency of micronucleus according to the whole control negative control group that we used BMAA was not statistically significant compared with the EMS (positive control group) (p>0.05). However, no significant cytotoxic effect was found when the results of the NDI were examined (p>0.05). 

Cyanobacteria, Genotoxicity, BMAA, Micronucles
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Orcid: 0000-0001-7849-4459
Yazar: Arif AYAR (Sorumlu Yazar)
Kurum: Amasya University, Sabuncuoğlu Şerefeddin Health Services Vocational School, Amasya
Ülke: Turkey


Yazar: Deniz ALTUN COLAK
Kurum: Erzincan Binali Yıldırım University, Faculty of Art and Science, Department of Biology, Erzincan
Ülke: Turkey


Yazar: Handan UYSAL
Kurum: Atatürk University, Faculty of Science, Department of Biology, Erzurum
Ülke: Turkey


Yazar: Vahit KONAR
Kurum: Amasya University, Faculty of Art and Science, Department of Biology, Amasya
Ülke: Turkey


Destekleyen Kurum Amasya University
Proje Numarası FMB-BAP 16-0205
Tarihler

Yayımlanma Tarihi : 30 Ağustos 2019

Bibtex @araştırma makalesi { sshs598328, journal = {Sabuncuoglu Serefeddin Health Sciences}, issn = {2667-6338}, address = {Amasya Üniversitesş Sabuncuoğlu Şerefeddin Sağlık Hizmetleri Meslek Yüksek Okulu İpekköy/Amasya}, publisher = {Amasya Üniversitesi}, year = {2019}, volume = {1}, pages = {8 - 14}, doi = {}, title = {Genotoxic and Cytotoxic Activity of Cyanobacterial (Blue-Green Algal) Toxin BMAA in Human Lymphocyte Cells}, key = {cite}, author = {AYAR, Arif and ALTUN COLAK, Deniz and UYSAL, Handan and KONAR, Vahit} }
APA AYAR, A , ALTUN COLAK, D , UYSAL, H , KONAR, V . (2019). Genotoxic and Cytotoxic Activity of Cyanobacterial (Blue-Green Algal) Toxin BMAA in Human Lymphocyte Cells. Sabuncuoglu Serefeddin Health Sciences , 1 (1) , 8-14 . Retrieved from https://dergipark.org.tr/tr/pub/sshs/issue/48459/598328
MLA AYAR, A , ALTUN COLAK, D , UYSAL, H , KONAR, V . "Genotoxic and Cytotoxic Activity of Cyanobacterial (Blue-Green Algal) Toxin BMAA in Human Lymphocyte Cells". Sabuncuoglu Serefeddin Health Sciences 1 (2019 ): 8-14 <https://dergipark.org.tr/tr/pub/sshs/issue/48459/598328>
Chicago AYAR, A , ALTUN COLAK, D , UYSAL, H , KONAR, V . "Genotoxic and Cytotoxic Activity of Cyanobacterial (Blue-Green Algal) Toxin BMAA in Human Lymphocyte Cells". Sabuncuoglu Serefeddin Health Sciences 1 (2019 ): 8-14
RIS TY - JOUR T1 - Genotoxic and Cytotoxic Activity of Cyanobacterial (Blue-Green Algal) Toxin BMAA in Human Lymphocyte Cells AU - Arif AYAR , Deniz ALTUN COLAK , Handan UYSAL , Vahit KONAR Y1 - 2019 PY - 2019 N1 - DO - T2 - Sabuncuoglu Serefeddin Health Sciences JF - Journal JO - JOR SP - 8 EP - 14 VL - 1 IS - 1 SN - 2667-6338- M3 - UR - Y2 - 2019 ER -
EndNote %0 Sabuncuoglu Serefeddin Health Sciences Genotoxic and Cytotoxic Activity of Cyanobacterial (Blue-Green Algal) Toxin BMAA in Human Lymphocyte Cells %A Arif AYAR , Deniz ALTUN COLAK , Handan UYSAL , Vahit KONAR %T Genotoxic and Cytotoxic Activity of Cyanobacterial (Blue-Green Algal) Toxin BMAA in Human Lymphocyte Cells %D 2019 %J Sabuncuoglu Serefeddin Health Sciences %P 2667-6338- %V 1 %N 1 %R %U
ISNAD AYAR, Arif , ALTUN COLAK, Deniz , UYSAL, Handan , KONAR, Vahit . "Genotoxic and Cytotoxic Activity of Cyanobacterial (Blue-Green Algal) Toxin BMAA in Human Lymphocyte Cells". Sabuncuoglu Serefeddin Health Sciences 1 / 1 (Ağustos 2019): 8-14 .
AMA AYAR A , ALTUN COLAK D , UYSAL H , KONAR V . Genotoxic and Cytotoxic Activity of Cyanobacterial (Blue-Green Algal) Toxin BMAA in Human Lymphocyte Cells. SSHS. 2019; 1(1): 8-14.
Vancouver AYAR A , ALTUN COLAK D , UYSAL H , KONAR V . Genotoxic and Cytotoxic Activity of Cyanobacterial (Blue-Green Algal) Toxin BMAA in Human Lymphocyte Cells. Sabuncuoglu Serefeddin Health Sciences. 2019; 1(1): 14-8.