Araştırma Makalesi
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Yıl 2019, Cilt 1, Sayı 1, 8 - 14, 30.08.2019

Öz

Kaynakça

  • Al-Sammak, M.A. 2012. Occurrence and effect of algal neurotoxins in Nebraska freshwater ecosystems. PhD. Thesis. University of Nebraska-Lincoln, USA.
  • Buenz, E. J., and Howe, C.L. 2007. Beta-methylarnino-alanine (BMAA) injures hippocampal neurons in vivo. Neurotoxicology, 28(3): 702-704.
  • Chang, Y.C., Chiu, S.-J., and Kao, K.P. 1993. Beta-N-methylamino-L-alanine (L-BMAA) decreases brain glutamate receptor number and induces behavioral changes in rats. The Chinese journal of physiology, 36(2): 79-84.
  • Cox, P.A., Kostrzewa, R.M., Guillemin, G.J. 2017. BMAA and neurodegenerative illness. Neurotox. Res.,1: 1-6.
  • Dawson, R., Marschall, E.G., Chan, K.C., Millard, W.J., Eppler, B., and Patterson, T.A. 1998. Neurochemical and neurobehavioral effects of neonatal administration of β-N-methylamino-l-alanine and 3,3'-iminodipropionitrile. Neurotoxicology and Teratology, 20(2): 181-192.
  • de Munck, E., Muñoz-Sáez, E., Miguel, B.G., Solas, M.T., Ojeda, I., Martínez, A., et al. 2013. β-N-methylamino-l-alanine causes neurological and pathological phenotypes mimicking Amyotrophic Lateral Sclerosis (ALS): The first step towards an experimental model for sporadic ALS. Environmental toxicology and pharmacology, 36(2): 243-255.
  • Esterhuizen-Londt, M., Pflugmacher, S., and Downing, T.G. 2011. β-N-Methylamino-L-alanine (BMAA) uptake by the aquatic macrophyte Ceratophyllum demersum. Ecotoxicology and Environmental Safety, 74(1): 74-77.
  • Esterhuizen-Londt, M., Pflugmacher, S., and Downing, T.G. 2011b. The effect of β-N-methylamino-L-alanine (BMAA) on oxidative stress response enzymes of the macrophyte Ceratophyllum demersum. Toxicon, 57(5): 803-810.
  • Fenech, M. 2000. The In vitro micronucleus technique. Mutation Research, 455 (1-2): 81-95.
  • Fleming L.E., Rivero C., Burns J., Willams C., Bean J.A., Shea K.A., Stinn J. 2002. Blue green algal (cyanobacterial) toxins, surface drinking water, and liver cancer in Florida. Harmful Algae, 1: 157-168.
  • Geh, E.N., Armah, A., Ghosh, D., Stelma, G., & Bernstein, J.A. 2016. Sensitization of a child to Cyanobacteria after recreational swimming in a lake. Journal of Allergy and Clinical Immunology, 137(6): 1902-1904.
  • Gibbons, C.F., & LeBaron, M. J. 2017. Applied genetic toxicology: From principles to practice. Environmental and molecular mutagenesis, 58(5): 232-234.
  • Güven, G.S., Cuna, T., Birinci, N., Güven, M., Onaran, İ. , Hacıhanefioğlu, S. ,Ulutin, T. 2006. 17- β Estradiole ile İndüklenen İnsan Lenfositlerindeki Mikronükleus Sıklığının İncelenmesi. Cerrahpaşa Tıp Dergisi, 37: 10-13.
  • Jonasson, S., Eriksson, J., Berntzon, L., Rasmussen, U., and Bergman, B. 2008. A novel cyanobacterial toxin (BMAA) with potential neurodegenerative effects. Plant biotechnology, 25(3): 227-232.
  • Karlsson, O., Berg, A. L., Lindstrom, A.K., Hanrieder, J., Arnerup, G., Roman, E., et al. 2012. Neonatal exposure to the cyanobacterial toxin BMAA induces changes in protein expression, and neurodegeneration in adult hippocampus. Toxicological Sciences, 130(2): 391-404.
  • Karlsson, O., Lindquist, N.G., Brittebo, E.B., and Roman, E. 2009a. Selective brain uptake and behavioral effects of the cyanobacterial toxin BMAA (β-N-methylamino-l-alanine) following neonatal administration to rodents. Toxicological Sciences, 109(2): 286-295.
  • Karlsson, O., Roman, E., and Brittebo, E.B. 2009b. Long-term cognitive impairments inadult rats treated neonatally with β-N-methylamino-L-alanine Toxicological Sciences, 112(1): 185-195.
  • Karlsson, O., Roman, E., Berg, A.L., and Brittebo, E.B. 2011. Early hippocampal cell death, and late learning and memory deficits in rats exposed to the environmental toxin BMAA (β-N-methylamino-L-alanine) during the neonatal period. Behavioural Brain Research, 219(2): 310-320.
  • Lürling, M., Faassen, E.J., and Van Eenennaam, J.S. 2011. Effects of the cyanobacterial neurotoxin β-N-methylamino-L-alanine (BMAA) on the survival, mobility and reproduction of Daphnia magna. Journal of Plankton Research, 33(2): 333-342.
  • Matsuoka, Y., Rakonczay, Z., Giacobini, E., and Naritoku, D. (1993). L-beta-methylamino-alanine-induced behavioral changes in rats. Pharmacology Biochemistry and Behavior, 44(3): 727-734.
  • Melo Cavalcante, A.A., Rubensam, G., Picada, J.N., Gomes da Silva, E., Fonseca Moreira, J.C., & Henriques, J.A. 2003. Mutagenicity, antioxidant potential and antimutagenic activity against hydrogen peroxide of cashew (Anacardium occidentale) apple juice and cajuina. Environmental and molecular mutagenesis, 41(5): 360-369.
  • Novak, M., Hercog, K., & amp; Egura, B. 2016. Assessment of the mutagenic and genotoxic activity of cyanobacterial toxin beta-N-methyl-amino-L-alanine in Salmonella typhimurium. Toxicon, 118: 134-140.
  • Okle, O., Rath, L., Galizia, C.G., and Dietrich, D.R. 2013. The cyanobacterial neurotoxin beta-N-methylamino-L-alanine (BMAA) induces neuronal and behavioral changes in honey bees. Toxicology and Applied Pharmacology, 270(1): 9-15.
  • Purdie, E.L., Samsudin, S., Eddy, F.B., and Codd, G.A. 2009. Effects of the cyanobacterial neurotoxin β-N-methylamino-L-alanine on the early-life stage development of zebrafish (Danio rerio). Aquatic Toxicology, 95(4): 279-284.
  • Santucci, S., Zsurger, N., and Chabry, J. 2009. β-N-methylamino-l-alanine induced in vivo retinal cell death. Journal of Neurochemistry, 109(3): 819-825.
  • Şekeroğlu, V., & Atlı-Şekeroğlu, Z. (2011). Genotoksik hasarın belirlenmesinde mikronükleus testi. Turk Hij Den Biyol Derg, 68(4): 241-252.
  • Smith, S.E., and Meldrum, B.S. 1990. Receptor site specificity for the acute effects of β-N-methylamino-alanine in mice. European Journal of Pharmacology, 187(1): 131-134.
  • Svirčev, Z.B., Tokodi, N., Drobac, D., & Codd, G.A. 2014. Cyanobacteria in aquatic ecosystems in Serbia: effects on water quality, human health and biodiversity. Systematics and biodiversity, 12(3): 261-270.
  • Young, R.R. 2002. Genetic toxicology: web resources. Toxicology, 173(1-2): 103-121.
  • Zeiger, E. 2004. History and rationale of genetic toxicity testing: an impersonal, and sometimes personal, view. Environmental and molecular mutagenesis, 44(5): 363-371.
  • Zhou, X.C., Escala, W., Papapetropoulos, S., Bradley, W.G., and Zhai, R.G. 2009. BMAA neurotoxicity in Drosophila. Amyotrophic Lateral Sclerosis, 10(S2): 61-66.
  • Zhou, X.C., Escala, W., Papapetropoulos, S., and Zhai, R.G. 2010. β-N-methylamino-L-alanine induces neurological deficits and shortened life span in Drosophila. Toxins, 2(11): 2663-2679.

Genotoxic and Cytotoxic Activity of Cyanobacterial (Blue-Green Algal) Toxin BMAA in Human Lymphocyte Cells

Yıl 2019, Cilt 1, Sayı 1, 8 - 14, 30.08.2019

Öz

Cyanobacteria, one of the oldest life forms, are also known as blue-green algae. Many species of cyanobacteria produce metabolites called cyanotoxin, which are classified as hepatotoxins, neurotoxins and cytotoxins and are highly toxic to vertebrate organisms. One of these toxins is the Beta-N-methyl-amino-L-alanine (BMAA). Blooms (large numbers or colonies) of the cyanobacteria or related organisms produce one or more toxins that can be dangerous to fish, wild animals and humans. There is not enough information in the literature related to the genotoxic effect of this toxin. In this study, it was aimed to determine investigation of the cytotoxic and genotoxic activity of cyanobacterial toxin BMAA in human lymphocyte cells by micronucleus assay. Different concentrations (20, 10, 5 and 1 μg/ml) of BMAA cyanotoxin were applied to human lymphocyte cell culture and micronucleus frequencies (MN) and nuclear division index (NDI) were calculated to determine genotoxic and cytotoxic effects. The data obtained from our study were compared with the negative control group prepared with dimethyl sulfoxide (DMSO) and with the well known ethyl methanesulfonate (EMS) with genotoxic effect and positive control group. According to these results; increasing the frequency of micronucleus according to the whole control negative control group that we used BMAA was not statistically significant compared with the EMS (positive control group) (p>0.05). However, no significant cytotoxic effect was found when the results of the NDI were examined (p>0.05). 

Kaynakça

  • Al-Sammak, M.A. 2012. Occurrence and effect of algal neurotoxins in Nebraska freshwater ecosystems. PhD. Thesis. University of Nebraska-Lincoln, USA.
  • Buenz, E. J., and Howe, C.L. 2007. Beta-methylarnino-alanine (BMAA) injures hippocampal neurons in vivo. Neurotoxicology, 28(3): 702-704.
  • Chang, Y.C., Chiu, S.-J., and Kao, K.P. 1993. Beta-N-methylamino-L-alanine (L-BMAA) decreases brain glutamate receptor number and induces behavioral changes in rats. The Chinese journal of physiology, 36(2): 79-84.
  • Cox, P.A., Kostrzewa, R.M., Guillemin, G.J. 2017. BMAA and neurodegenerative illness. Neurotox. Res.,1: 1-6.
  • Dawson, R., Marschall, E.G., Chan, K.C., Millard, W.J., Eppler, B., and Patterson, T.A. 1998. Neurochemical and neurobehavioral effects of neonatal administration of β-N-methylamino-l-alanine and 3,3'-iminodipropionitrile. Neurotoxicology and Teratology, 20(2): 181-192.
  • de Munck, E., Muñoz-Sáez, E., Miguel, B.G., Solas, M.T., Ojeda, I., Martínez, A., et al. 2013. β-N-methylamino-l-alanine causes neurological and pathological phenotypes mimicking Amyotrophic Lateral Sclerosis (ALS): The first step towards an experimental model for sporadic ALS. Environmental toxicology and pharmacology, 36(2): 243-255.
  • Esterhuizen-Londt, M., Pflugmacher, S., and Downing, T.G. 2011. β-N-Methylamino-L-alanine (BMAA) uptake by the aquatic macrophyte Ceratophyllum demersum. Ecotoxicology and Environmental Safety, 74(1): 74-77.
  • Esterhuizen-Londt, M., Pflugmacher, S., and Downing, T.G. 2011b. The effect of β-N-methylamino-L-alanine (BMAA) on oxidative stress response enzymes of the macrophyte Ceratophyllum demersum. Toxicon, 57(5): 803-810.
  • Fenech, M. 2000. The In vitro micronucleus technique. Mutation Research, 455 (1-2): 81-95.
  • Fleming L.E., Rivero C., Burns J., Willams C., Bean J.A., Shea K.A., Stinn J. 2002. Blue green algal (cyanobacterial) toxins, surface drinking water, and liver cancer in Florida. Harmful Algae, 1: 157-168.
  • Geh, E.N., Armah, A., Ghosh, D., Stelma, G., & Bernstein, J.A. 2016. Sensitization of a child to Cyanobacteria after recreational swimming in a lake. Journal of Allergy and Clinical Immunology, 137(6): 1902-1904.
  • Gibbons, C.F., & LeBaron, M. J. 2017. Applied genetic toxicology: From principles to practice. Environmental and molecular mutagenesis, 58(5): 232-234.
  • Güven, G.S., Cuna, T., Birinci, N., Güven, M., Onaran, İ. , Hacıhanefioğlu, S. ,Ulutin, T. 2006. 17- β Estradiole ile İndüklenen İnsan Lenfositlerindeki Mikronükleus Sıklığının İncelenmesi. Cerrahpaşa Tıp Dergisi, 37: 10-13.
  • Jonasson, S., Eriksson, J., Berntzon, L., Rasmussen, U., and Bergman, B. 2008. A novel cyanobacterial toxin (BMAA) with potential neurodegenerative effects. Plant biotechnology, 25(3): 227-232.
  • Karlsson, O., Berg, A. L., Lindstrom, A.K., Hanrieder, J., Arnerup, G., Roman, E., et al. 2012. Neonatal exposure to the cyanobacterial toxin BMAA induces changes in protein expression, and neurodegeneration in adult hippocampus. Toxicological Sciences, 130(2): 391-404.
  • Karlsson, O., Lindquist, N.G., Brittebo, E.B., and Roman, E. 2009a. Selective brain uptake and behavioral effects of the cyanobacterial toxin BMAA (β-N-methylamino-l-alanine) following neonatal administration to rodents. Toxicological Sciences, 109(2): 286-295.
  • Karlsson, O., Roman, E., and Brittebo, E.B. 2009b. Long-term cognitive impairments inadult rats treated neonatally with β-N-methylamino-L-alanine Toxicological Sciences, 112(1): 185-195.
  • Karlsson, O., Roman, E., Berg, A.L., and Brittebo, E.B. 2011. Early hippocampal cell death, and late learning and memory deficits in rats exposed to the environmental toxin BMAA (β-N-methylamino-L-alanine) during the neonatal period. Behavioural Brain Research, 219(2): 310-320.
  • Lürling, M., Faassen, E.J., and Van Eenennaam, J.S. 2011. Effects of the cyanobacterial neurotoxin β-N-methylamino-L-alanine (BMAA) on the survival, mobility and reproduction of Daphnia magna. Journal of Plankton Research, 33(2): 333-342.
  • Matsuoka, Y., Rakonczay, Z., Giacobini, E., and Naritoku, D. (1993). L-beta-methylamino-alanine-induced behavioral changes in rats. Pharmacology Biochemistry and Behavior, 44(3): 727-734.
  • Melo Cavalcante, A.A., Rubensam, G., Picada, J.N., Gomes da Silva, E., Fonseca Moreira, J.C., & Henriques, J.A. 2003. Mutagenicity, antioxidant potential and antimutagenic activity against hydrogen peroxide of cashew (Anacardium occidentale) apple juice and cajuina. Environmental and molecular mutagenesis, 41(5): 360-369.
  • Novak, M., Hercog, K., & amp; Egura, B. 2016. Assessment of the mutagenic and genotoxic activity of cyanobacterial toxin beta-N-methyl-amino-L-alanine in Salmonella typhimurium. Toxicon, 118: 134-140.
  • Okle, O., Rath, L., Galizia, C.G., and Dietrich, D.R. 2013. The cyanobacterial neurotoxin beta-N-methylamino-L-alanine (BMAA) induces neuronal and behavioral changes in honey bees. Toxicology and Applied Pharmacology, 270(1): 9-15.
  • Purdie, E.L., Samsudin, S., Eddy, F.B., and Codd, G.A. 2009. Effects of the cyanobacterial neurotoxin β-N-methylamino-L-alanine on the early-life stage development of zebrafish (Danio rerio). Aquatic Toxicology, 95(4): 279-284.
  • Santucci, S., Zsurger, N., and Chabry, J. 2009. β-N-methylamino-l-alanine induced in vivo retinal cell death. Journal of Neurochemistry, 109(3): 819-825.
  • Şekeroğlu, V., & Atlı-Şekeroğlu, Z. (2011). Genotoksik hasarın belirlenmesinde mikronükleus testi. Turk Hij Den Biyol Derg, 68(4): 241-252.
  • Smith, S.E., and Meldrum, B.S. 1990. Receptor site specificity for the acute effects of β-N-methylamino-alanine in mice. European Journal of Pharmacology, 187(1): 131-134.
  • Svirčev, Z.B., Tokodi, N., Drobac, D., & Codd, G.A. 2014. Cyanobacteria in aquatic ecosystems in Serbia: effects on water quality, human health and biodiversity. Systematics and biodiversity, 12(3): 261-270.
  • Young, R.R. 2002. Genetic toxicology: web resources. Toxicology, 173(1-2): 103-121.
  • Zeiger, E. 2004. History and rationale of genetic toxicity testing: an impersonal, and sometimes personal, view. Environmental and molecular mutagenesis, 44(5): 363-371.
  • Zhou, X.C., Escala, W., Papapetropoulos, S., Bradley, W.G., and Zhai, R.G. 2009. BMAA neurotoxicity in Drosophila. Amyotrophic Lateral Sclerosis, 10(S2): 61-66.
  • Zhou, X.C., Escala, W., Papapetropoulos, S., and Zhai, R.G. 2010. β-N-methylamino-L-alanine induces neurological deficits and shortened life span in Drosophila. Toxins, 2(11): 2663-2679.

Ayrıntılar

Birincil Dil İngilizce
Konular Temel Bilimler
Bölüm Research Articles
Yazarlar

Arif AYAR (Sorumlu Yazar)
Amasya University, Sabuncuoğlu Şerefeddin Health Services Vocational School, Amasya
0000-0003-0473-4653
Türkiye


Deniz ALTUN COLAK
Erzincan Binali Yıldırım University, Faculty of Art and Science, Department of Biology, Erzincan
Türkiye


Handan UYSAL
Atatürk University, Faculty of Science, Department of Biology, Erzurum
Türkiye


Vahit KONAR Bu kişi benim
Amasya University, Faculty of Art and Science, Department of Biology, Amasya
Türkiye

Destekleyen Kurum Amasya University
Proje Numarası FMB-BAP 16-0205
Yayımlanma Tarihi 30 Ağustos 2019
Başvuru Tarihi 29 Temmuz 2019
Kabul Tarihi 29 Ağustos 2019
Yayınlandığı Sayı Yıl 2019, Cilt 1, Sayı 1

Kaynak Göster

APA Ayar, A. , Altun Colak, D. , Uysal, H. & Konar, V. (2019). Genotoxic and Cytotoxic Activity of Cyanobacterial (Blue-Green Algal) Toxin BMAA in Human Lymphocyte Cells . Sabuncuoglu Serefeddin Health Sciences , 1 (1) , 8-14 . Retrieved from https://dergipark.org.tr/tr/pub/sshs/issue/48459/598328