HYOSCYAMUS RETICULATUS’UN HEKZAN VE SU ÖZÜTLERİNİN ANTİOKSİDAN VE ANTİMİKROBİYAL ÖZELLİKLERİ ÜZERİNE BİR ÇALIŞMA

Yıl 2014, Sayı: 39, 21 - 29, 01.12.2014

Erdoğan Güneş Ahmet Uysal Abdurrahman Aktümsek Yusuf Durak

Öz

Bu çalışmanın amacı Hyoscyamus reticulatus’dan elde edilen hekzan ve su özütlerinin antioksidan kapasitelerini ve antimikrobiyal etkilerini belirlemektir. Antioksidan kapasite radikal süpürme (DPPH testi), toplam antioksidan kapasite, demir ve bakır indirgeme testlerini içeren dört farklı test sistemi ile araştırıldı. Antimikrobiyal etki sıvı mikro dilüsyon metodu ile değerlendirildi. Fosfomolibdat testi (total antioksidan kapasite) dışında, su özütünün hekzan özütünden daha yüksek antioksidan aktiviteye sahip olduğu belirlenmiştir. Toplam fenolik miktarı hekzan ve su özütünde sırasıyla 15.86 mg GAE/g ve 24.25 mg GAE/g olarak bulunmuştur. Ek olarak, hekzan özütü su özütüne kıyasla önemli bir antimikrobiyal etki göstermiştir. Bu çalışma ile elde edilen sonuçlar açıkça Hyoscyamus reticulatus’un oksidatif ve enfeksiyöz süreçlerde, doğal ajanların bir kaynağı olarak önemli bir potansiyele sahip olduğunu göstermiştir.

Anahtar Kelimeler

Hyoscyamus, Antioksidan, Antimikrobiyal, Fenolik, Türkiye

A STUDY ON ANTIOXIDANT AND ANTIMICROBIAL PROPERTIES OF HEXANE AND WATER EXTRACTS FROM HYOSCYAMUS RETICULATUS

Öz

The aim of this work was to determine antioxidant capacities and antimicrobial effects of hexane and water extracts from Hyoscyamus reticulatus. The antioxidant capacity was screened by four different test systems including radical scavenging (DPPH assay), total antioxidant capacity, ferric and cupric reducing powers. Antimicrobial effects were evaluated with broth micro dilution method. It was determined that water extract had higher antioxidant activity than hexane extract, except for phosphomolybdenum assay (total antioxidant capacity). Amount of total phenolics in hexane and water extracts were found as 15.86 mgGAE/g and 24.25 mgGAE/g, respectively. Moreover, hexan extract has exhibited significant an antimicrobial effect as compared to water extract. Results obtained in this work clearly indicate that Hyoscyamus reticulatus has significant potential as a source of natural agents for the management of oxidative and infectious process.

Anahtar Kelimeler

Hyoscyamus, Antioxidant, Antimicrobial, Phenolic, Turkey

Kaynakça

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