Seedling Response of Two Barley Cultivars and Gamma Ray-Induced Advanced Barley Lines to Rhynchosporium commune

Öz

Barley scald caused by Rhynchosporium commune is an important disease of barley (Hordeum vulgare). Barley mutants could be used for enhancing genetic diversity in barley. In this study, barley cultivar Tokak 157/37 was subjected to gamma irradiation using Cobalt- 60 as the radiation source and mutant barleys were obtained. Under greenhouse conditions, twenty-five advanced mutant barley lines (M₈ generation) and two barley cultivars (Tokak 157/37 and Bülbül 89) were tested for their resistance status to 3 Rhynchosporium commune single spore isolates obtained from Gaziantep, Eskişehir and Manisa provinces of Turkey. For evaluation of the lines and cultivars a 0-4 scale was used. Response of lines to isolates ranged between highly resistant and highly susceptible. Isolate obtained from Gaziantep was the most virulent followed by isolates obtained from Manisa and Eskişehir. Mutant barley line # 20 showed a resistant reaction to Gaziantep isolate. Mutant barley lines # 7, 13, 20, 21, 23 and 25 showed a highly resistant reaction to Eskişehir isolateand line #16 showed a resistant reaction to this isolate. Lines # 7 and 25 exhibited resistant and intermediate reactions to Manisa isolate, respectively. The other mutant barley lines showed susceptible and highly susceptile reactions to isolates. Barley cultivars Tokak 157/37 and Bülbül 89 exhibited highly susceptible reactions to all 3 isolates.

Anahtar Kelimeler

• Rhynchosporium commune; Hordeum vulgare; disease resistance; mutant barley lines; Cobalt-60 irradiation

İki Arpa Çeşidinin ve Gamma Işınlaması ile Elde Edilmiş İleri Kademe Arpa Hatlarının Rhynchosporium commune’ a Fide Dönemi Tepkileri

Öz

Rhynchosporium commune tarafından meydana getirilen arpa yaprak lekesi hastalığı arpa (Hordeum vulgare) bitkilerinin önemli bir hastalığıdır. Arpa mutantları arpada genetik çeşitliliği zenginleştirmek için kullanılabilir. Bu çalışmada Tokak 157/37 arpa çeşidinin Kobalt-60 kaynağında gamma ışınlarıyla ışınlanması sonucu geliştirilen M₈ generasyon kademesindeki 25 mutant arpa hattının ve iki arpa çeşidinin (Tokak 157/37 ve Bülbül 89) Gaziantep, Eskişehir ve Manisa’dan elde edilen 3 Rhynchosporium commune tek spor izolatına sera koşullarında dayanıklılık durumları incelenmiştir. Çeşit ve hatların değerlendirilmesi için 0-4 ıskalası kullanılmıştır. Hatların izolatlara tepkileri yüksek derecede dayanıklı ile yüksek derecede hassas arasında değişmiştir. Gaziantep’den elde edilen izolat en virülent izolat olarak bulunmuş ve bu izolatı Manisa ve Eskişehir izolatları takip etmiştir. Yirmi numaralı mutant arpa hattı Gaziantep izolatına dayanıklı tepki vermiştir. Yedi, 13, 20, 21, 23 ve 25 numaralı mutant arpa hatları Eskişehir izolatına yüksek derecede dayanıklı tepki verirken 16 numaralı hat dayanıklı tepki vermiştir. Yedi numaralı mutant arpa hattı Manisa izolatına dayanıklı tepki verirken 25 numaralı hat Manisa izolatına orta derecede dayanıklı tepki vermiştir. Diğer mutant arpa hatları izolatlara hassas ile yüksek derecede hassas tepkiler vermişlerdir. Tokak 157/37 ve Bülbül 89 çeşitleri 3 izolata da yüksek derecede hassas tepki vermişlerdir.

Anahtar Kelimeler

• Rhynchosporium commune; Hordeum vulgare; hastalıklara dayanıklılık; mutant arpa hatları; Kobalt-60 ışınlaması

Kaynakça

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