Effect of Different Sowing Times on Leaf Characteristics and Indican Content of Some Isatis Species

Öz

In this study, effects of different sowing dates on the some agronomic properties of 4 Isatis species (Isatis tinctoria L., Isatis candolleana BOISS. (endemic), Isatis tinctoria L. subsp. corymbosa (BOISS.) DAVIS and Isatis buschiana SCHISCHKIN were investigated in ecological conditions of Kahramanmaras in 2008 and 2009 growing season. Optimum sowing time for I. tinctoria leaf yield was spring and for the other species were autumn. The highest leaf yield was obtained in 2008 for all species and the values were 15185 kg ha^-1 in I. tinctoria, 15109 kg ha^-1, 19299 and 10909 kg ha^-1 in I. tinctoria subsp. corymbosa, I. buschiana and I. candolleana, respectively. The highest indican contents were found 23.5, 21.4, 18.2 and 15.2 mg g^-1 in I. buschiana, I. tinctoria subsp. corymbosa, I. tinctoria and I. candolleana, respectively, by using ultrasonic extraction and DAD detector analysis of HPLC.

Anahtar Kelimeler

• HPLC-DAD,Isatis,indican,leaf yield

Bazı Isatis Türlerinin Yaprak Karakterleri ve İndikan İçeriği Üzerine Farklı Ekim Zamanlarının Etkisi

Öz

Bu çalışmada Kahramanmaraş ekolojik koşullarında 2008 ve 2009 yetiştirme sezonlarında yetiştirilen 4 Isatis türünün (Isatis tinctoria, Isatis candolleana (endemik), Isatis tinctoria subsp. corymbosa ve Isatis buschiana) bazı agronomik özellikleri üzerine farklı ekim zamanlarının etkileri incelenmiştir. I. tinctoria bitkisinden yaprak verimi almak için optimum ekim zamanı ilkbaharken diğer türler için sonbahar olmuştur. I. tinctoria’dan 15185 kg ha^-1 , I. tinctoria subsp. corymbosa’dan 15109 kg ha^-1, I. buschiana’dan 19299 ve I. candolleana’dan 10909 kg ha^-1  olmak üzere, tüm türler için en yüksek yaprak verimleri 2008 yılında elde edilmiştir. HPLC’de yapılan analizler sonucunda, I. buschiana, I. tinctoria subsp. corymbosa, I. tinctoria ve I. candolleana’da en yüksek indikan içerikleri sırasıyla 23.5, 21.4, 18.2 ve 15.2 mg g^-  olmak üzere ultrasonikasyonla yapılan ve DAD dedektörle yapılan okumalardan elde edilmiştir.

Anahtar Kelimeler

• Ekim zamanı,HPLC-DAD,Isatis,indikan

Kaynakça

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