Ferric reductase activity and production of phytosiderophore in fresh roots of rice varieties grown under iron deficiency conditions and chlorosis degrees in leaves

Öz

The aim of this study is to determine ferric reductase activity and production of phytosiderophore in fresh roots of rice varıeties
grown under iron deficiency conditions and chlorosis degrees in leaves In order to determine phytosiderophore secretion
production and ferric reductase activity, rice varieties were grown in sand culture by applying iron-free nutrient solution for 10
days. In addition, a 5×2 factorial trial design was conducted to determine the degree of chlorosis and the tolerance index value to
chlorosis in rice varieties. In this study, a nutrient solution containing Fe-EDDHA in doses of 0 and 45 µM Fe was applied to 5
different rice varieties. Among the rice varieties, the most phytosiderophores produced in fresh roots were found in the Hamzadere
rice variety. The difference between the remaining varieties in terms of phytosiderophore production in the roots was statistically
insignificant and was found to be close to each other. Among the rice varieties grown under iron deficiency conditions, it was
determined that Ronaldo variety had the highest ferric reductase activity in fresh roots, whereas the lowest variety was Biga İncisi
variety. The degree of chlorosis observed in the leaves of Hamzadere and Ronaldo rice varieties was found to be severe, and the
tolerance index values of these varieties for iron deficiency were calculated as 41.24% and 41.52%, respectively. According to these
results, it was determined that the most susceptible variety to iron deficiency among the rice varieties examined in the study was
Hamzadere rice variety.

Anahtar Kelimeler

• Rice variety
• phytosiderophore
• ferric reductase activity
• SPAD meter
• chlorosis degree

Demir noksanlığı şartlarında yetiştirilen çeltik çeşitlerinin taze köklerinde fitosiderofor üretimi ve ferrik redüktaz aktivitesi ile yapraklarında kloroz dereceleri

Öz

Bu çalışmanın amacı, demir noksanlığı şartlarında yetiştirilen çeltik çeşitlerinin taze köklerinde fitosiderofor üretimi ve ferrik
redüktaz aktivitesi ile yapraklarında kloroz derecelerini belirlemektir. Fitosiderofor salgısı üretimi ve ferrik redüktaz aktivitesi
belirlemek amacıyla çeltik çeşitleri 10 gün süreyle demirsiz besin çözeltisi uygulayarak kum kültüründe yetiştirilmiştir. Ayrıca,
kloroz derecesinin ve kloroza tolerans indeks değerinin belirlenmesi amacıyla 5×2 faktöriyel deneme deseninde çalışma
yürütülmüş olup, bu çalışmada 5 farklı çeltik çeşidine 0 ve 45 µM Fe dozlarında Fe-EDDHA içeren besin çözeltisi uygulanmıştır. Taze
kökte üretilen fitosiderofor miktarı en yüksek olan çeşidin Hamzadere çeltik çeşidi olduğu görülmüştür. Köklerde fitosiderofor
üretimleri bakımından geri kalan diğer çeşitler arası fark istatistiksel olarak önemsiz olup, birbirlerine yakın bulunmuştur. Demir
noksanlığı şartlarında yetiştirilen çeltik çeşitleri arasında taze köklerde ferrik redüktaz aktivitesi en yüksek olan çeşidin Ronaldo
çeltik çeşidi olduğu; buna karşın, en düşük çeşidin ise Biga incisi çeltik çeşidi olduğu görülmüştür. Hamzadere ve Ronaldo çeltik
çeşitlerinin yapraklarında gözlenen kloroz derecesi şiddetli olup, bu çeşitlerin demir noksanlığına tolerans indeks değerleri
sırasıyla %41.24 ve %41.52 olarak hesaplanmıştır. Bu sonuçlara göre, araştırmada incelenen çeltik çeşitleri arasında demir
noksanlığına en hassas çeşidin Hamzadere çeltik çeşidi olduğu belirlenmiştir.

Anahtar Kelimeler

• Çeltik çeşidi
• Fitosiderofor
• Ferrik redüktaz aktivitesi
• SPAD metre
• Kloroz derecesi

Kaynakça

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