The effect of toxic level iron application on fresh root with cold extractable iron and zinc content and Fe/Zn ratio in some rice varieties

Öz

The aim of this study is to determine the effect of toxic iron content complete nutrient solution application on cold extractable iron
and zinc content and Fe / Zn ratio in fresh root in some rice cultivars. 5 different rice varieties were used in the study. These
varieties used in the study are Biga İncisi, Osmancık-97, Hamzadere, Ronaldo and Edirne. Complete nutrient solution in the form of
iron sulphate heptahydrate, i) 45 μM Fe (sufficient Fe), ii) 3.50 mM Fe (toxic Fe), iii) 3.50 mM Fe (containing 10 % activated
bentonite) was applied to the rice varieties grown in sand culture. Fresh roots of rice varieties were extracted with dithionitecitrate-bicarbonate (DSB) solution, and iron and zinc concentrations in this extract were determined in atomic absorption
spectrophotometer device. Toxic iron application increased cold extractable iron, zinc content and Fe/Zn ratio in fresh root.
Extractable Fe, Zn content and Fe / Zn ratio in fresh roots in bentonite-added medium were found to be lower than in fresh roots of
plants grown in bentonite-free medium. Fe and Zn uptake decreased in fresh roots in bentonite-added sand media. Rice varieties
grown with the application of a toxic level of iron containing nutrient solution in bentonite-free sand media listed as Osmancık-97 >
Ronaldo > Edirne > Hamzadere > Biga İncisi in terms of cold extractable iron content values in fresh roots, while cold extractable
zinc content values listed as Osmancık-97 > Hamzadere > Edirne > Biga İncisi ~ Ronaldo. Fe/Zn ratio values in fresh roots of paddy
varieties grown with the application of a nutrient solution containing toxic levels of iron in bentonite-free sand environment listed
as Ronaldo ~ Edirne > Biga İncisi > Osmancık-97 > Hamzadere. It was determined that the zinc content of Ronaldo and Edirne
varieties decreased more than other varieties under iron toxicity conditions.

Anahtar Kelimeler

• Rice variety
• extractable iron and zinc with dithionite-citrate-bicarbonate in fresh root
• iron toxicity

Bazı çeltik çeşitlerinde toksik düzeyde demir uygulamasının taze kökte soğuk ekstrakte edilebilir demir ve çinko kapsamı ile Fe/Zn oranına etkisi

Öz

Bu çalışmanın amacı, çeltik çeşitlerinde toksik düzeyde demir içerikli tam besin çözeltisi uygulamasının taze kökte soğuk ekstrakte
edilebilir demir ve çinko kapsamı ile Fe/Zn oranına etkisini belirlemektir. Çalışmada 5 farklı çeltik çeşidine kum kültüründe demir
sülfat heptahidrat formunda, i) 45 μM Fe (yeterli Fe), ii) 3.50 mM Fe (toksik-Fe), iii) 3.50 mM Fe (% 10 aktive bentonit içerikli kum
ortamı) konsantrasyonlarında demir içeren tam besin çözeltisi uygulanmıştır. Denemede kullanılan bazı çeltik çeşitleri: Biga incisi,
Osmancık-97, Hamzadere, Ronaldo ve Edirne’dir. Çeltik çeşitlerinin taze kökleri dithionite-sitrat-bikarbonat (DSB) çözeltisi ile
ekstrakte edilmiş, ve bu ekstraktaki demir ve çinko konsantrasyonları atomik absorpsiyon spektrofotometre cihazında
belirlenmiştir. Toksik düzeyde demir uygulaması taze kökte soğuk ekstrakte edilebilir demir, çinko kapsamını ve Fe/Zn oranını
arttırmıştır. Bentonit ilaveli ortamda taze köklerde ekstrakte edilebilir Fe, Zn kapsamı ve Fe/Zn oranı bentonitsiz ortamda
yetiştirilen bitkilerin taze köklerindekine göre daha düşük bulunmuştur. Bentonitli ortamda taze köklerde Fe ve Zn tutulumu
azalma göstermiştir. Bentonitsiz kum ortamında toksik düzeyde demir içeren besin çözeltisi uygulaması ile yetiştirilen çeltik
çeşitleri taze kökte soğuk ekstrakte edilebilir demir kapsam değerleri bakımında Osmancık-97 > Ronaldo > Edirne > Hamzadere >
Biga incisi şeklinde sıralanmış; soğuk ekstrakte edilebilir çinko kapsam değerleri Osmancık-97 > Hamzadere > Edirne > Biga incisi
~ Ronaldo şeklinde sıralanmış; taze kökte Fe/Zn oran değerleri ise Ronaldo ~ Edirne > Biga incisi > Osmancık-97 > Hamzadere
şeklinde sıralanmıştır. Ronaldo ve Edirne çeşitlerinde demir toksikliği ortamında çinko kapsamının daha fazla azaldığı tespit
edilmiştir.

Anahtar Kelimeler

• Çeltik çeşidi
• taze kökte dithionite-sitrat-bikarbonat ile ekstrakte edilebilir demir ve çinko
• Demir toksisitesi

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