Domates bakteriyel solgunluk ve kanser hastalığının (Clavibacter michiganensis subsp. michiganensis (Smith) Davis et al.) bakteriyel antagonistler kullanılarak mücadele imkânlarının araştırılması

Öz

Domates bakteriyel solgunluk ve kanser hastalığı (Clavibacter michiganensis subsp. michiganensis (Smith) Davis et al.) Dünyada olduğu gibi Türkiye’de de domates yetiştiriciliğinde önemli kayıplara neden olmaktadır. Bu patojene karşı; yapılan kültürel önlemler ve kimyasal mücadele yöntemlerinin yetersiz olduğu bilinmektedir. Bu çalışmada, toplam 1150 potansiyel antagonist bakteri izolatı patojene karşı antibakteriyel aktivitesi için Petri denemelerinde test edilmiştir. Etkili bulunan toplam 48 izolatın bazı biyokimyasal özellikleri, ürettikleri metabolitleri, enzim aktiviteleri belirlenmiş; sıvı besi ortamında geliştirilen bu izolatların saksı denemelerinde hastalığı engelleme oranları tespit edilmiştir. Test edilen tüm izolatların hastalığın gelişimini % 6.5 ile 93.4 oranında engellediği tespit edilmiştir. Hastalığı engelleme oranına göre en yüksek etkiyi Bacillus megaterium TV-49A (%93.4) ve B. cereus TV-85D (%86.9) bakteri izolatları göstermiştir. Bu iki izolatın ACC, indol asetik asit, salisilik asit üretimi ve fosfatı çözebilme özelliğinin yüksek olduğu; lipaz, amilaz, proteaz, liziz dekarboksilaz, fitaz ve kitinaz enzim üretimi sonuçlarının pozitif olduğu belirlenmiştir.

Anahtar Kelimeler

• Bakteriyel solgunluk ve kanser
• biyolojik mücadele
• Clavibacter michiganensis subsp. michiganensis
• domates

Evaluation of biological control possibilities for tomato bacterial wilt and canker (Clavibacter michiganensis subsp. michiganensis (Smith) Davis et al.) by using bacterial biocontrol agents

Öz

Tomato bacterial wilt and canker disease (Clavibacter michiganensis subsp. michiganensis (Smith) cause significant losses in tomato cultivation in both Turkiye and worldwide. Cultural measures and chemical control methods have limited success against this pathogen. In this study, 1150 potential biocontrol agent bacterial strains were tested for activity against this pathogen in Petri dish experiments. Biochemicals, metabolites and enzymes from a total of 48 strains were effective against the pathogen. The disease prevention rates of these potential biocontrol agents, which were propagated in liquid media, were examined in pot trials. The tested strains reduced the development of the diseases by from 6.5% to 93.4%. Bacillus megaterium TV-49A (93.4%) and B. cereus TV-85D (86.9%) showed the highest effects in terms of disease prevention. These isolates produce ACC, indole acetic acid and salicylic acid, and high phosphate solubility. Furthermore, they were positive for lipase, amylase, protease, lysis decarboxylase, phytase and chitinase enzyme production.

Anahtar Kelimeler

• Bacterial wilt and canker
• biological control
• Clavibacter michiganensis subsp. michiganensis
• tomato

Kaynakça

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