Araştırma Makalesi

In vitro evaluation of the antifungal activity of plant essential oils against entomopathogenic fungi

Cilt: 17 Sayı: 2026 29 Eylül 2026
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In vitro evaluation of the antifungal activity of plant essential oils against entomopathogenic fungi

Öz

Entomopathogenic fungi, particularly Beauveria bassiana, are promising biological control agents, whereas plant-derived essential oils are receiving increasing attention because of their insecticidal and repellent properties. However, interactions between these biological agents remain insufficiently understood. In this study, vapor-phase and contact effects of thyme, rosemary, and eucalyptus oils applied at 1% and 3% were evaluated against B. bassiana BMAUM M6-4 and BbEMRKZAkar-1. Antifungal activity was assessed based on mycelial growth inhibition and conidial germination. Conidia produced after essential-oil exposure were also tested against Tenebrio molitor larvae. The 3% thyme-oil treatment generally produced the strongest inhibition of mycelial growth, reaching 83.8% under contact exposure. Effects on conidial germination depended on isolate and exposure method. In the bioassay, terminal mortality ranged from 50.0% to 76.7% after contact exposure and from 66.7% to 96.7% after vapor-phase exposure. However, treatment effects were not significant within either exposure method (P>0.05). The 3% thyme-contact bioassay was not performed because conidial production was insufficient to prepare the required suspension concentration. Overall, the effects of the essential oils on B. bassiana varied according to oil type, concentration, fungal isolate, and exposure method. Although essential-oil exposure affected fungal growth and conidial germination under some conditions, it did not significantly alter the subsequent pathogenicity of BbEMRKZAkar-1 against T. molitor. These findings provide a preliminary assessment of the compatibility between the tested essential oils and B. bassiana and support further laboratory investigation before their potential combined use is evaluated under semi-field or field conditions.

Anahtar Kelimeler

Destekleyen Kurum

the Scientific Research Projects Coordination Unit of Kahramanmaraş Sütçü İmam University

Proje Numarası

2022/6-19 LAP

Etik Beyan

his study did not involve human participants or vertebrate animals. The research consisted of laboratory experiments conducted using fungal cultures and larvae of Tenebrio molitor, an invertebrate insect species. Therefore, ethical committee approval was not required for this study. All stages of the research were conducted in accordance with the principles of scientific research and publication ethics.

Teşekkür

This study was supported by the Scientific Research Projects Coordination Unit of Kahramanmaraş Sütçü İmam University as an undergraduate research project under Grant No. 2022/6-19 LAP. The authors also thank Ayşegül Orçan for her valuable assistance with the laboratory work.

Kaynakça

  1. Abbott W.S., 1925. A method of computing the effectiveness of an insecticide. Journal of Economic Entomology, 18 (2): 265–267.
  2. Abdel-Kader M.M., N.S. El-Mougy, S.M. Lashin & H.H. Abdel-Kader, 2011. Essential oils and Trichoderma harzianum as integrated control measure against faba bean root rot pathogens. Archives of Phytopathology and Plant Protection, 44(14): 1362–1373.
  3. Arnason J.T, B.J.R. Philogene & P. Morand, 1989. Insecticides of Plant Origin. ACS Symp Ser. No 387. American Chemical Society, Washington, Dc, USA, 213 p.
  4. Aydın T., M. Branco, Ö. Güven, H. Gonçalves, A. Lima, İ. Karaca & T.M. Butt, 2018. Significant mortality of eggs and young larvae of two pine processionary moth species due to the entomopathogenic fungus Metarhizium brunneum. Biocontrol Sciencea and Technology, 28(4): 317-331.
  5. Batish D.R., H.P. Singh, R.K. Kohli & S. Kaur, 2008. Eucalyptus essential oil as natural pesticide. Forest Ecology and Management, 256: 2166-2174.
  6. Baydar R., Ö. Güven & İ. Karaca, 2016. Occurence of Entomopathogenic Fungi in Agricultural Soils from Isparta Province in Turkey and Their Pathogenicity to Galleria mellonella L (Lepidoptera: Pyralidae) Larvae. Egyptian Journal of Biological Pest Control, 26(2), 323-327.
  7. Bozhüyük A.U. & Ş. Kordali, 2019. Ocimum basilicum L. uçucu yağının fitopatojenik funguslar üzerine antifungal etkileri. Erciyes Tarım ve Hayvan Bilimleri Dergisi, 2(1), 45-57.
  8. Butt T.M., L. Ibrahim, S.J. Clark & A. Beckett, 1995. The germination behavior of Metarhizium anisopliae on the surface of aphid and flea beetle cuticles. Mycological Research, 99: 945–950.

Ayrıntılar

Birincil Dil

İngilizce

Konular

Bitki Koruma (Diğer)

Bölüm

Araştırma Makalesi

Yayımlanma Tarihi

29 Eylül 2026

Gönderilme Tarihi

18 Ağustos 2026

Kabul Tarihi

11 Eylül 2026

Yayımlandığı Sayı

Yıl 2026 Cilt: 17 Sayı: 2026

Kaynak Göster

APA
(kalkar) Güven, O., Kara, E., & Altun, D. (2026). In vitro evaluation of the antifungal activity of plant essential oils against entomopathogenic fungi. Türkiye Biyolojik Mücadele Dergisi, 17(2026), 1-12. https://doi.org/10.31019/tbmd.2020439
AMA
1.(kalkar) Güven O, Kara E, Altun D. In vitro evaluation of the antifungal activity of plant essential oils against entomopathogenic fungi. Türk. biyo. müc. derg. 2026;17(2026):1-12. doi:10.31019/tbmd.2020439
Chicago
(kalkar) Güven, Ozlem, Emine Kara, ve Duygu Altun. 2026. “In vitro evaluation of the antifungal activity of plant essential oils against entomopathogenic fungi”. Türkiye Biyolojik Mücadele Dergisi 17 (2026): 1-12. https://doi.org/10.31019/tbmd.2020439.
EndNote
(kalkar) Güven O, Kara E, Altun D (01 Eylül 2026) In vitro evaluation of the antifungal activity of plant essential oils against entomopathogenic fungi. Türkiye Biyolojik Mücadele Dergisi 17 2026 1–12.
IEEE
[1]O. (kalkar) Güven, E. Kara, ve D. Altun, “In vitro evaluation of the antifungal activity of plant essential oils against entomopathogenic fungi”, Türk. biyo. müc. derg, c. 17, sy 2026, ss. 1–12, Eyl. 2026, doi: 10.31019/tbmd.2020439.
ISNAD
(kalkar) Güven, Ozlem - Kara, Emine - Altun, Duygu. “In vitro evaluation of the antifungal activity of plant essential oils against entomopathogenic fungi”. Türkiye Biyolojik Mücadele Dergisi 17/2026 (01 Eylül 2026): 1-12. https://doi.org/10.31019/tbmd.2020439.
JAMA
1.(kalkar) Güven O, Kara E, Altun D. In vitro evaluation of the antifungal activity of plant essential oils against entomopathogenic fungi. Türk. biyo. müc. derg. 2026;17:1–12.
MLA
(kalkar) Güven, Ozlem, vd. “In vitro evaluation of the antifungal activity of plant essential oils against entomopathogenic fungi”. Türkiye Biyolojik Mücadele Dergisi, c. 17, sy 2026, Eylül 2026, ss. 1-12, doi:10.31019/tbmd.2020439.
Vancouver
1.Ozlem (kalkar) Güven, Emine Kara, Duygu Altun. In vitro evaluation of the antifungal activity of plant essential oils against entomopathogenic fungi. Türk. biyo. müc. derg. 01 Eylül 2026;17(2026):1-12. doi:10.31019/tbmd.2020439