Efficacy of entomopathogenic nematode isolates from Turkey and Kyrgyzstan against bean weevil [Acanthoscelides obtectus (Say) (Coleoptera: Chrysomelidae)] adults

Öz

In this study, the efficacies of two entomopathogenic nematode (EPN) isolates of both Heterorhabditis bacteriophora (11KG and TOK20) and Steinernema feltiae (3KG and Tokat-Emir), obtained from Turkey and Kyrgyzstan, against an important storage pest of beans, the bean weevil (Acanthoscelides obtectus Say. Coleoptera: Chrysomelidae), were determined. In single dose assays, the 2 isolates of S. feltiae caused the highest mortality (99.59%) of A. obtectus adults. At 3 different doses (25, 50 and 100 IJs insect-1) and temperatures (10, 15 and 20°C), the most effective isolates were H. bacteriophora TOK20 (73.09%), S. feltiae 3KG (100%) and S. feltiae 3KG (100%) at 100 IJs at 10°C, 15°C and 20°C, respectively. In the dose-mortality assays, the S. feltiae 3KG isolate was effective at 500 IJs insect-1.

Anahtar Kelimeler

• Bean
• Entomopathogenic nematodes
• Heterorhabditis
• Steinernema
• Bean weevil
• Acanthoscelides obtectus

Türkiye ve Kırgızistan’dan elde edilen entomopatojen nematodların fasulye tohum böceği [Acanthoscelides obtectus (Say) (Coleoptera: Chrysomelidae)]’ne karşı etkinliklerinin araştırılması

Öz

Bu çalışmada Türkiye ve Kırgızistan’a ait Entomopatojen nematod (EPN) izolatlarından Heterorhabditis bacteriophora türüne ait iki (11KG ve TOK20) ve Steinernema feltiae türüne ait iki (3KG ve Tokat-Emir) izolatın, depolanmış fasulye zararlısı olan fasulye tohum böceği (Acanthoscelides obtectus Say. Coleoptera: Bruchidae)’ne karşı etkinliği ortaya konmuştur. Tek doz denemeleri, en yüksek etkiyi S. feltiae’nin her 2 izolatı (3KG ve Tokat-Emir) göstermiştir (%99.59). 3 farklı doz (25, 50 ve 100 IJ böcek-1) ve sıcaklıkta (10, 15 ve 20°C) yürütülen deneme sonucunda en etkili izolatın 10°C’de 100 IJ dozunda H. bacteriophora TOK20 izolatı (%73,09), 15°C’de 100 IJ dozunda S. feltiae 3KG izolatı (%100) ve 20°C’de 100 IJ dozunda S. feltiae 3KG izolatı (%100) olduğu tespit edilmiştir. Doz-ölüm denemeleri sonucunda; A. obtectus’a karşı S. feltiae 3KG izolatının 500 IJ böcek-1 konsantrasyonunda en yüksek etkiye sahip olduğu ortaya konmuştur.

Anahtar Kelimeler

• Fasulye
• Entomopatojen nematod
• Heterorhabditis
• Steinerma
• Fasulye tohum böceği
• Acanthoscelides obtectus

Kaynakça

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